Enterovirus Universal (EV) Fluorescent PCR Detection Kit

Product NameEnterovirus Universal (EV) Fluorescent PCR Detection Kit

Name：Enterovirus Detection Kit （Real-Time PCR Method）

【 Packaging Specifications 】 25T/box, 50T/box

Hand, foot, and mouth disease is a common childhood illness caused by enteroviruses, mostly resulting in fever and water rash on the mouth, palms, and soles of the feet. A small number of patients may experience symptoms such as meningitis, encephalitis, neurogenic pulmonary edema, myocarditis, and even death. The enteroviruses that cause hand, foot, and mouth disease include Coxsackievirus, novel enterovirus, and enterovirus. Currently, laboratory diagnostic methods mainly include virus isolation and nucleic acid testing.

This kit is suitable for detecting enterovirus RNA in specimens, and is suitable for auxiliary diagnosis and epidemiological investigation of enterovirus infections. The test results are for reference only.

【 Principle of Inspection 】

This kit utilizes one-step reverse transcription polymerase chain reaction (RT-PCR) detection technology to design specific primers and Taqman for enteroviruses

Fluorescent probe for in vitro amplification and detection of enterovirus RNA, used for pathogen detection of suspected infectious materials in scientific research experiments.

【 Reagent Composition 】

Explanation: Components of different batch numbers of reagent kits cannot be used interchangeably.

Storage conditions and expiration date

-20 ℃± 5 ℃, stored away from light, transported, and subjected to repeated freeze-thaw cycles no more than 5 times, with a shelf life of 12 months.

ABI7500、 Agilent MX3000P/3005P, LightCycler, Bio Rad, Eppendorf and other series of fluorescence quantitative PCR detectors.

Symptoms of upper respiratory tract infection (fever, spray, runny nose, cough, etc.): Dip a sampling swab into nasal or pharyngeal secretions and place them in a sterile sampling tube (containing 0.4ml)

Sterilized physiological saline solution

Fecal: Dip a sampling swab into fecal or diarrheal material and place it in a bacterial sampling tube (containing 0.4ml of sterile physiological saline)

[Storage and Transportation]

The collected or processed samples should be stored at 2 ℃~8 ℃ for no more than 24 hours; if long-term storage is required, they should be stored at -70 ℃ or below, with no more than 3 freeze-thaw cycles.

【 Precautions 】

1.All operations must be strictly carried out in accordance with the instructions;

2.Before use, all components in the reagent kit should be naturally melted, thoroughly mixed, and briefly centrifuged;

3.The reaction solution should be stored away from light;

4.Try to avoid the presence of bubbles during the reaction, and cover the tube tightly;

5.Use disposable suction tips, disposable gloves, and specialized work clothes for each area;

6.Sample processing, reagent preparation, and sample addition should be carried out in different areas to avoid cross contamination;

7.After the experiment is completed, treat the workbench and pipette with 10% hypochlorous acid, 75% alcohol, or a UV lamp;

8.All items in the reagent kit should be treated as contaminants and handled in accordance with the "Biosafety Guidelines for Microbial Biomedical Laboratories".

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