Hair keratin 33A antibody

publicDepartmentThe product is only for scientific research purposesSpecially supplied antibodies are pure chemicals used in chemical reactions, analysis and testing, research experiments, teaching experiments, and chemical formulas. They are affordable, available in various specifications, and have after-sales service.

English name:KRT 33 A

English alias:Ha-3I; HA3I; Hairkeratin; Hairkeratin,typeIHa3-l; Hardkeratin,typeI,3I; hHa3-I; HHA3-l; HKA3A; K33A; Keratin; Keratin,hair,acidic,3A; Keratin,typeIcuticularHa3-I; Keratin-33A; Krt1-3; Krt33a; KRTHA3A; KT33A_HUMAN; typeIcuticularHa3-I; typeIHa3-I.

cross-reaction:Human, Mouse

mark:Unconjugated

Antibody source:Rabbit

Antibody type:Polyclonal

Immunogen:KLHconjugatedsyntheticpeptidederivedfromhumanKRT33A

Protein cell localization: cytoplasm, extracellular matrix, secreted proteins

Purification method:affinitypurifiedbyProteinA

subtype:IgG

trait:Liquid

concentration:1mg/ml

Storage liquid:0.01MTBS(pH7.4)with1%BSA,0.03%Proclin300and50%Glycerol.

Storage conditions:Shippedat4℃. Storeat-20°Cforoneyear. Avoidrepeatedfreeze/thawcycles.

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Classification of Animal Antibody Functions:
① Pig antibodies: swine fever antibodies, porcine blue ear antibodies, porcine circovirus antibodies, porcine pseudorabies antibodies, porcine parvovirus antibodies, porcine foot-and-mouth disease antibodies, porcine influenza antibodies, etc.
② Avian antibodies: gosling plague antibodies, duck liver antibodies, duck serositis antibodies, antibodies, Newcastle disease antibodies, etc
③ Cow antibodies: bovine foot-and-mouth disease antibodies, bovine mastitis antibodies, bovine fever antibodies, bovine viral diarrhea antibodies, bovine hemorrhagic sepsis antibodies, etc
④ Sheep antibodies: Sheep pox antibodies, Sheep foot-and-mouth disease antibodies, Sheep small ruminant disease antibodies, Sheep fast disease antibodies, Sheep intestinal toxemia antibodies, Sheep sudden gangrene antibodies, Sheep black disease antibodies, etc.
⑤ Dog antibodies: rabies antibodies, distemper antibodies, parainfluenza antibodies, adenovirus antibodies, and parvovirus disease antibodies in dogs, as well as pseudo rabies antibodies, parvovirus antibodies, and Japanese encephalitis antibodies in foxes, raccoons, and minks.

Experimental steps of immunofluorescence technology:

1、 Prepare reagents and instruments:

Phosphate buffered saline, fluorescently labeled antibody solution, buffered glycerol, three enamel buckets, one covered enamel box, fluorescence microscope, slide holder, filter paper37 ℃ temperature chamber, etc.

2、 Experimental steps

1. Add 0.01mol/L, pH 7.4 PBS dropwise onto the test specimen slide, discard after ten minutes, and maintain a certain humidity of the specimen.

2. Add appropriately diluted fluorescent labeled antibody solution dropwise to cover the specimen, place it in a covered enamel box, and keep it warm for a certain period of time, with a reference of thirty minutes.

3. Take out the glass slide, place it on the slide holder, rinse it with 0.01mol/L, pH 7.4 PBS first, and then soak it in three tanks of 0.01mol/L, pH 7.4 PBS in order. Each tank should be shaken and shaken continuously for three to five minutes.

4. Take out the glass slide and use filter paper to absorb excess water, but do not dry the specimen. Add a drop of buffer glycerol and cover the slide with a cover.

5. Immediately observe with a fluorescence microscope. Observe the specific fluorescence intensity of the specimen.

The preparation process of antibodies:

1. Preparation of immunogens

Ordinary macromolecular proteins can be cloned into vectors and induced for expression in Escherichia coli to obtain recombinant proteins. After purification and identification, they can be directly used as immunogens.

Small molecule proteins or compounds with small molecular weights require modification of the molecule with a coupling carrier to make it an immunogenic antigen. Common coupling carriers includeBSA, OVA, HAS, etc.

2. Immune animals

Animals commonly used for preparing anti serum include guinea pigs, rabbits, chickens, rats and mice, and dogs, sheep, goats, etc. are required for mass production.

3. Collection of immune serum

Generally, rabbits, sheep, and goats can use arterial blood collection, rabbits, guinea pigs, rats, and chickens can use cardiac blood collection, and rabbits, goats, and sheep can use venous blood collection.

4. Purification and identification of immune serum

The obtained serum needs further purification, using an affinity column coupled with the antigen for chromatography, which has high efficiency, strong specificity, and high purity. Next, the content, relative molecular weight, purity, and specificity of the purified protein need to be identified.

5. Preservation of immune serum

Suggest packaging the antibodies and storing them separately. Antibodies are generally relatively stable and-The temperature range of 80 ℃ to -20 ℃ can be stored for about 5 years without affecting the potency, while the vacuum drying storage time can be even longer. Before storage, it needs to be sterilized and preservatives added.

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