HAKATA ultra-low lgG fetal bovine serum (available for sale in small packages)

HAKATA ultra-low lgG fetal bovine serum (available for sale in small packages)

Product Name: Ultra LowLgG fetal bovine serum

English name:Ultra low IgG Fetal Bovine Serum

Item No.HC-FBS-50 and HC-FBS-500

Specifications50ML，50ML*10，500ML

Storage temperature:-20°C

Validity period: at least five years, avoiding repeated freeze-thaw cycles

Targeting customers: scientific research, enterprises, etc

Dialysis type fetal bovine serumIntroduction:

Serum is commonly used as an additive for cell growth and has multiple functions and uses, such as being rich in large molecular proteins, low molecular weight nutrients, and serving as an additional source of amino acids, carbohydrates, and vitamins; Improve the buffering capacity of the medium; Can combine or neutralize toxic ingredients; Reduce cellular oxidative stress and decrease cell apoptosis; Providing growth promoting factors helps cells adapt to the culture environment.

Conventional cell culture requires the addition of serum to maintain the proliferation ability of cells. Although serum can meet the general needs of cell culture, certain specific components still need to be removed in experiments that optimize nutrient composition and label specific components for research. The commonly used removal method is dialysis. Dialysis is the process of circulating serum through hollow fibers and supplementing physiological saline in the serum to maintain osmotic pressure, removing molecules with a molecular weight less than10000MW small molecules such as amino acids, glucose, salt ions, and unbound proteins.

Dialysis treatment using ultrafiltration can remove molecular weight less thanA molecule of 10000Da cannot remove hormones during the process, but may reduce the growth ability of some types of cells. Remove small molecules with a molecular weight below 10000Da and eliminate external interference; Very low endotoxin, sterile filtration, with the last three sterile filtration treatments at 0.1um; Conduct testing for bacteria, fungi, mycoplasma, viruses, and virus antibodies in accordance with international requirements.

Dialysis of fetal bovine serum is mainly used for cell culture, but it also has its own special applications, such as doping research, drug screening at the cellular level, and cell clone screening.

ultra-lowIgG fetal bovine serum, Ultra low IgG Introduction:

ultra-lowIgG fetal bovine serum only contains natural low-level IgG, which is the main immunoglobulin in animal serum. It is a natural serum component involved in immune function and can be transmitted through the placenta. Artificial removal of IgG can lead to a decrease in serum performance, while natural fetal bovine serum IgG can interfere with cell culture. The detailed description is as follows:

1. Ultra low endotoxin fetal bovine serum is prepared by screening for endotoxins below 0.1 EU/mL in fetal bovine plasma, followed by three rounds of 0.1um filtration and strict aseptic processes to ensure endotoxin levels are ≤ 0.1 EU/mL. This serum is particularly suitable for endotoxin sensitive experimental systems, such as immune cells, stem cells, primary cells, skin absorption models, and biopharmaceutical development.

2. Each batch of products is tested using the horseshoe crab reagent (LAL) colorimetric method, with endotoxin levels controlled at ≤ 0.1 EU/mL, and accompanied by a complete quality inspection report (COA).

3. The product significantly improves cell state stability and experimental reproducibility while maintaining the activity of serum growth factors, proteins, and nutrients. Its ultra-low endotoxin properties can effectively avoid cellular stress response, abnormal signaling pathways, and experimental data bias, making it an ideal choice in the fields of scientific research and biopharmaceuticals.

Tetracycline negative fetal bovine serumFetal Bovine Serum,Tetracycline free 介绍：

Tetracycline negative fetal bovine serumTetracycline Negative Fetal Bovine Serum typically contains the antibiotic tetracycline (tet), and researchers face unexpected expression issues when using highly sensitive tet induced gene expression systems. It is necessary for serum to contain the lowest level of tetracycline to ensure precise gene expression. Fetal bovine serum contains tetracycline, and researchers often encounter unexpected gene expression issues when using sensitive tetracycline induced regulation systems. To ensure precise gene expression, it is better to have as little tetracycline as possible in the serum. This product can be applied to cell culture using sensitive tetracycline induction expression systems, as well as other tetracycline sensitive experiments. The tetracycline detection content is about 0.001 µ g/ml.

Tetracycline negative fetal bovine serum is commonly used in scientific research, diagnostic reagents, vaccine production, and development. It can also be used as a blocking agent in immune reactions and a fixative in protein ligand reactions.

Tetracycline negative fetal bovine serum plays an important role in cell culture, especially in experiments that require precise control of gene expression. Regarding the comparative experiment on the pluripotency maintenance effect of tetracycline negative fetal bovine serum, the following are some analyses:

1、 Characteristics of tetracycline negative fetal bovine serum

Tetracycline negative fetal bovine serum refers to serum that has undergone special treatment to ensure that its tetracycline content is extremely low (usually below the detection limit, such as...)0.001? G/ml) fetal bovine serum. This serum is suitable for cell culture using sensitive tetracycline induced expression systems, as well as other tetracycline sensitive experiments. Its features include sterile filtration, mycoplasma testing, virus screening, and specific storage conditions (-20 ° C to -10 ° C).

2、 Experimental design for comparing the maintenance effects of pluripotency

To evaluate the effectiveness of tetracycline negative fetal bovine serum in maintaining pluripotency, the following comparative experiments can be designed:

1. Experimental group: Cultivate pluripotent stem cells using fetal bovine serum.

2. Control group: Cultivate pluripotent stem cells using regular fetal bovine serum (which may contain tetracycline).

3、 Experimental steps

1. Cell preparation: Select pluripotent stem cells in good condition for cultivation.

2. Serum addition: Tetracycline negative fetal bovine serum was added to the experimental group, while ordinary fetal bovine serum was added to the control group.

3. Cultivation conditions: Maintain the same cultivation conditions, such as temperature, humidity, CO2 concentration, etc.

4. Observation indicators: Regularly observe cell morphology, proliferation rate, and expression of multipotent marker genes.

5. Data collection and analysis: Collect experimental data and conduct statistical analysis to evaluate the effect of fetal bovine serum on maintaining pluripotency.

4、 Expected results and conclusions

1. Expected results: The experimental group cells may exhibit better performance in maintaining pluripotency, such as more stable cell morphology, faster proliferation rate, and higher expression levels of pluripotency marker genes.

2. Conclusion: Tetracycline negative fetal bovine serum helps maintain the pluripotency of pluripotent stem cells and is suitable for cell culture experiments that require precise control of gene expression.

5、 Precautions

1. Serum quality: Ensure that the quality of fetal bovine serum and regular fetal bovine serum meets the experimental requirements.

2. Experimental conditions: Strictly control the experimental conditions to reduce the influence of external factors on the experimental results.

3. Data accuracy: Ensure the accuracy and reliability of experimental data for accurate statistical analysis and conclusion inference.

Tetracycline negative fetal bovine serum may have better efficacy in maintaining pluripotency than regular fetal bovine serum. However, the specific effect still needs to be verified through rigorous comparative experiments. In practical applications, appropriate serum types should be selected based on experimental requirements and cell characteristics.

Carbon adsorption of fetal bovine serumCharcoal Stripped FBS

1. Carbon adsorption fetal bovine serum: It is a low-level steroid hormone fetal bovine serum product. The serum is sourced from healthy fetal bovine serum collected aseptically; GMP purification workshop production, with 3 rounds of 0.1 micron sterilization. During the production process, carbon adsorption and D-glucoside are used to remove hormones from serum.

2. Scope of application: Cultivate stem cells, primary cells, nerve cells, human cancer cells, human bone marrow cells, leukemia cells, human embryonic lung fibroblasts, human brain nerve cells, tumor cells, and other cells.

3. Characteristics:

(1) Carbon adsorption of fetal bovine serum for in vitro culture to verify the effect of hormones;

(2) The research includes steroid receptor linkage, cell receptor steroid regulation, various tissue hormone secretion, and thyroid hormone function;

(3) During the production process, charcoal and D-glucose are used to remove hormones from the serum. Carbon adsorption can reduce the concentration of many hormones and growth factors in serum.

4. Advantages:

4.1 Composition aspect

(1) Reducing the concentration of interfering molecules: By using carbon adsorption, the concentration of many hormones and growth factors in serum can be effectively reduced, avoiding their interference with cell culture in experiments. This is suitable for studies where these molecules may interfere with experimental results, such as eliminating the influence of these substances that originally exist in serum when studying the response of certain cells to specific hormones or growth factors.

(2) Low endotoxin content: Typically, after three rounds of nanoscale filtration, endotoxins can be effectively removed, reducing the adverse effects on cell culture and lowering the probability of cells aging, apoptosis, and other conditions caused by endotoxin. This helps to improve the accuracy of experimental results.

4.2 Quality control aspects

(1) Small batch differences: The production process strictly controls batch differences to ensure stable serum quality across different batches, resulting in good reproducibility of experimental results and facilitating long-term experimental research and data comparison between different laboratories.

(2) Reliable and traceable source: The serum source is clear and reliable, and corresponding inspection and quarantine reports can be provided for each batch, which facilitates tracking and understanding of the quality and safety of the serum, making it more reassuring for researchers to use.

4.3 Cell culture aspect

(1) Suitable for a variety of cell types: Suitable for various conventional cell cultures, such as stem cells, primary cells, nerve cells, etc., providing a suitable growth environment for the in vitro culture of various cells.

(2) Beneficial for cell growth: It can provide good growth conditions for cells, promote cell adhesion and proliferation. For example, in some cell culture experiments, using carbon to adsorb fetal bovine serum can enable cells to better adhere to the wall and maintain high proliferation activity.

HAKATA ultra-low lgG fetal bovine serum (available for sale in small packages)

serial number product Item Number specification official price

new product Standard fetal bovine serum QC-FBS-500 500ML 1600

new product Fetal bovine serum (New Zealand source) NZ-FBS-500 500ML 6500

new product Tetracycline negative fetal bovine serum HD-FBS-500 500ML 5600

new product Tetracycline negative fetal bovine serum (no packaging required) HD-FBS-50 50ML*10 5800

new product Low fat fetal bovine serum HL-FBS-500 500ML 5600

new product Low fat fetal bovine serum HL-FBS-50 50ML*10 5800

new product Bovine serum albumin（BSA） H-BSA-500 500g 1950

new product Bovine serum albumin（BSA） H-BSA-1000 1000g 3750

new product Bovine serum albumin（7.5%) Cell culture grade H-BSA-CELL 100ML 390

1 Characterized FBS HN-FBS-500 500ML 2750

2 Characterized FBS(No need for packaging) HN-FBS-50 50ML*10 2850

3 Defined FBS HB-FBS-500 500ML 4750

4 Defined FBS(No need for packaging) HB-FBS-50 50ML*10 4890

5 Stem cell specific fetal bovine serum HS-FBS-500 500ML 6200

6 Stem cell specific fetal bovine serum(No need for packaging) HS-FBS-50 50ML*10 6500

7 Dialysis type fetal bovine serum HX-FBS-500 500ML 5600

8 Dialysis type fetal bovine serum(No need for packaging) HX-FBS-50 50ML*10 5800

9 Activated carbon adsorption to remove estrogen from fetal bovine serum HT-FBS-500 500ML 5600

10 Activated carbon adsorption to remove estrogen from fetal bovine serum(No need for packaging) HT-FBS-50 50ML*10 5800

11 ultra-lowLgG fetal bovine serum HC-FBS-500 500ML 5100

12 ultra-lowLgG fetal bovine serum (no packaging required) HC-FBS-50 50ML*10 5300

13 Heat inactivated serum HR-FBS-500 500ML 3300

14 Heat inactivated serum(No need for packaging) HR-FBS-50 50ML*10 3500

15 No extracellular vesicle serum H - Exo - 50 50ML 2625

16 Newborn bovine serum HA-FBS-500 500ML 1100

17 Horse serum HM-FBS-500 500ML 1100

18 Goat serum HG-FBS-500 500ML 1550

19 Diagnostic reagents using calf serum (for industrial customers) HZ-FBS-500 500ML 1050

Definition of pure fetal bovine serum: