Guangzhou roundworm IgG antibody detection kit

Guangzhou roundworm IgG antibody detection kit

Common name: Guangzhou roundworm IgG antibody detection kit
English name: Angiostrogylus Cantonensis (A.c) IgG Antibody Test Kit
48 servings/box, 96 servings/box
This kit uses indirect ELISA method to qualitatively detect IgG antibodies against Guangzhou roundworm in human serum or plasma
Body.
If the sample to be tested contains IgG antibodies against Guangzhou roundworm, it will react with the Guangzhou roundworm on the coating plate
Specific binding of the anti human secondary antibody enzyme-linked immunosorbent assay (ELISA) conjugate added in the first and second steps. Indirect binding of anti human secondary antibody enzyme-linked immunosorbent assay on the coated plate
The color development of the reaction between the conjugate and the substrate can reflect whether the sample contains IgG antibodies against Guangzhou roundworm.
Main components
Number Name Specification (48T/96T) Number Name Specification
1 package of plate 48/96 hole 7 termination liquid (No. 6 liquid) 6mL x 1 bottle
2 Enzyme conjugates (Solution 1) 3/6mL x 1 bottle 8 Positive control 0.8mL x 1 vial
3 Concentrated Wash Solution (No.2 Solution) 100mL x 1 bottle 9 Negative Control 0.8mL x 1 vial
4 substrates (solution No. 3) 6mL x 1 bottle, 10 self sealing bags, 1 piece
5 color developer (No. 4 solution) 6mL x 1 bottle 11 sealing plate film 2/3 pieces
6 sample dilutions (Solution No. 5) 100mL x 1 bottle 12 instruction manual 1 copy
【 Storage conditions and shelf life 】 Store in the dark at 2-8 ℃ to prevent freezing, with a shelf life of 12 months.

Enzyme linked immunosorbent assay (ELISA) with wavelengths of 450nm and 630nm; 37 ℃ constant temperature equipment; Adjustable single/multi channel micropipette.

1. This reagent is suitable for detecting human serum or plasma;
To achieve optimal experimental results, avoid using samples containing sodium azide, hyperlipidemia, bacterial growth, and severe hemolysis;
3. Serum samples to be tested in the short term (within 3 days) can be stored at 2-8 ℃; If long-term storage is required, it should be placed at -20 ℃
Or below, avoid repeated freezing and thawing.

1. Preparation of washing solution: Dilute the concentrated washing solution (No. 2 solution) with distilled water or deionized water at a ratio of 1:10 (for example, take
Mix 100mL of washing solution (10 x) with 900mL of distilled or deionized water, and thoroughly mix to obtain the working concentration for washing
Liquid.
2. Sample dilution: Dilute the serum to be tested with sample diluent (solution 5) at a ratio of 1:100 (e.g. 5 μ L serum+495 μ L)
Dilute the sample thoroughly. Negative and positive control samples do not need to be diluted, they can be added directly.
3. Sample addition reaction: Add only 100 μ L of the test sample to each well of the sample well; Blank control 2 wells, with only 100 μ L added to each well
Sample diluent; Two wells of negative control were added, with only 100 μ L of negative control added to each well; Positive control 2 wells, each well only
Add 100 μ L of positive control substance. Cover the sealing film and react in the dark at 37 ℃ for 30 minutes.
4. Plate washing: Shake off the liquid in the holes and fill each hole with (if using a washing machine, add 250 μ L to each hole) working concentration washing solution for washing
Each time, you need to stay for 1 minute; The next time, shake it clean and pat it dry.
5. Enzyme addition reaction: except for the blank control well, add 1 drop of enzyme conjugate (solution 1) to each well, cover with a sealing film, and incubate at 37 ℃
React in the dark for 30 minutes. Remove the liquid from the hole, wash the board as above, and pat dry.
6. Color reaction: Add 1 drop of substrate (solution 3) and 1 drop of color reagent (solution 4), mix well, cover with a sealing film, and avoid at 37 ℃
Light color development takes 10 minutes.
7. Termination reaction: Add 1 drop of termination solution (solution No. 6), zero with blank control, and read the OD value using an enzyme-linked immunosorbent assay reader at a wavelength of 450nm (630nm as the reference wavelength).

1. Cut off value (COV)=average OD value of negative control x 2.1 (when the OD value of negative control wells is less than 0.07, it is calculated as 0.07).
2. Validity assessment:
Negative control well OD value ≤ 0.10 (if greater than 0.10, the experiment is invalid);
Positive control well OD value ≥ 0.50 (if less than 0.50, the experiment is invalid).
Explanation of Inspection Results
If the OD value of the sample is ≥ COV, it is judged as positive for IgG antibodies against Guangzhou roundworm;
If the OD value of the sample is less than COV, it is judged as negative for IgG antibodies against Guangzhou roundworm.
Limitations of the testing method: This kit is for research purposes only and is not intended for clinical diagnosis;
【 Precautions 】
1. The wrapping board must be sealed to prevent moisture. When removed from the refrigerated environment, it should be equilibrated to room temperature before opening and using. Unused
The finished Flat noodles shall be immediately put back into the self sealing bag with desiccant, sealed and stored at 2~8 ℃.
Before adding the reagent, gently shake and mix the reagent bottle.
3. The bottle cap should be tightly closed after each use and should not be mixed between different bottle caps. The reagent components of different reagent kits cannot be mixed.
4. When incubating, it is recommended to use a water bath. If there is no water bath box, a sealed wet box can be placed in a 37 ℃ temperature chamber for use.
5. Discarded reagent kits and samples should be properly disposed of as contaminants. The termination solution in the reagent kit is corrosive and should be used with caution.
Guangzhou roundworm IgG antibody detection kit