Gemini 100-512 human AB serum

Gemini 100-512 individuals with AB serum

Product brand:Gemini

Origin: United States

Item No.100-512

Product Name: HumanAB serum, normal human AB serum, AB human serum， human AB serum

Specifications100 mL/bottle

quality guarantee period:-Below 10 ℃; Valid for 5 years (if produced in 2025, valid until 2030)

Supplier: Shanghai Huiying Biotechnology Co., Ltd

Inventory status: In stock, sufficient dry ice transportation

Gemini 100-512is aLack of resistanceA/B antibodies, low immunogenicity, reduce heterologous/allogeneic immune reactionsSerum from the United StatesFDA registration agency, healthy AB type male donor, mixed>100 donors, endotoxin<0.75 EU/mL; Hemoglobin<20 mg/dL; Virus testing (HIV/HBV/HCV, etc.) is negative. Mainly used for: human cell culture (stem cells, primary cells, immune cells), immunology, oncology, virology research, and other research. Compared with fetal bovine serum, human sources are closer to the in vivo microenvironment, supporting faster growth of human cells and lower concentrations.

Usage suggestion:

Concentration: commonly used5-10% (lower than the commonly used 10-20% for FBS)

Storage requirements:

1. Long term storage: -20 ℃, sealed away from light

2. Do not place it at -80 ℃, as it can easily cause protein structure damage and layered precipitation

3. After opening and packaging, the small bottle should be stored at -20 ℃, and priority should be given to immediate use

Unfreezing precautions：

1. It is strictly prohibited to thaw directly at room temperature, as it may cause protein precipitation and increase in precipitation.

2. Standard thawing process:

Thaw overnight in the refrigerator at 2-8 ℃ first

Gently invert and mix every 1-2 hours during the process, without shaking vigorously

3. After melting, package separately to avoid repeated freezing and thawing of the entire bottle, with a maximum freezing and thawing limit of no more thanTwice.

Basic considerations for preparation and cultivation：

1. Conventional addition ratio: 5% to 10% is sufficient, there is no need to add 15% to 20% like fetal bovine serum

2. When preparing the solution, add serum at the end and do not mix it with high salt, strong acid, strong alkali reagents in advance

3. Filtration and sterilization: Commercially available with 0.1 μ m sterile filtration, there is no need for secondary filtration as excessive filtration can result in loss of growth factors

Taboos in Cell Culture Practice：

1. Not suitable for highly sensitive serum-free domesticated cells, need to replace with non heterologous human AB serum

2. If there is a small amount of slight protein flocculent precipitation: a normal phenomenon, it does not affect the culture; Discard a large amount of turbidity, blackening, and odor directly

3. The heat inactivated version has already inactivated complement, so there is no need to inactivate it by yourself in a water bath. Secondary heating will destroy the active factor

Precautions for use:

Leave it at room temperature for too long and then freeze it back Activity greatly reduced

Shake violently to produce foam Protein denaturation

And ordinaryFetal Bovine SerumMixing ratios and adding randomly Unstable cell growth state

Repeated freeze-thaw cycles Easy to fragment and undergo cell apoptosis

Gemini 100-512 individuals with AB serum

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