Fluorescence in situ hybridization FISH analysis software

1 Fluorescence in situ hybridization (FISH) analysis systemSoftware functional parameters:

1. *Qualification: The fluorescence in situ hybridization (FISH) analysis system has obtained the Medical Device Registration Certificate and Medical Device Production License of the People's Republic of China issued by the National Medical Products Administration. The structure and composition of the Medical Device Registration Certificate include fluorescence image processing（FISH）Module;

2. Various color fluorescence image acquisition, infinite color addition and correction, infinite color signal and image synthesis;

3. Support automatic coloring after grayscale acquisition, and the final synthesis effect can be seen in real-time during the processing of each channel, automatically synthesizing multi-color fluorescent images;

4. Support depth of focus extensionZAxis imaging (multifocal depth of field superposition, focal length extension)(EDF)）Function to obtain high-quality images even for thick specimens (tissue specimens);

5. A probe that is applicable to conventional microscopic fluorescence images and meets all color requirements;

6. Support image post-processing, editing, background processing, and signal point strength processing;

7. Weak fluorescence signal intelligent effect enhancement, enhancing fluorescence signal to remove background impurities, universal for interphase/Mid term split phaseFISHLocate the signal in the diagram;

8. *The software can automatically and accurately identify solid tumor cells, and can edit cell contours as needed;

9. *Support data statistics for various types of fluorescence analysis, providing commonly used analysis results for user reports, including mean, copy number, positivity ratio, and proportion of various signal types;

10. Support one click automatic adjustment of highlights and shadows, reducing the intensity of manual adjustment processing;

11. Support custom probe channel configuration;

12. *Storage is not less than320Commonly used dye configuration, supporting direct selection for use;

13. Accurately adjust the deviation of each image signal generated by the conversion filter on a pixel by pixel basis;

14. The sequence images with incorrect channel selection support easy adjustment;

15. supportROIAnd image export, image export supports resizing;

16. Real time browsing, collection, and saving of under the microscope images, with various processing, measurement, and annotation functions for the images;

17. Provide fine-grained user permission management to adapt to the permission control of doctors at different levels. Report trace backend recording and traceability query function;

18. *Provide no less than13Commonly used for histopathological diagnosis of various tissuesFISHGene template;

19. *Provide no less than10Used for the diagnosis of common blood diseasesFISHGene structured template, supports structured data export and can be directly used for big data statistics;

20. Support professional scientific research query and statistics, facilitating the citation of statistical data in papers;

21. Support built-in electronic signature, which can be directly used for self-service printing;

22. Support exportPDFFormat report, convenient for academic exchange and importLISSystem;

23. Support reports on various bodily fluids such as pathological tissues, bone marrow, peripheral blood, cerebrospinal fluid, pleural and peritoneal fluid, sputum, urine, etc;

24. *Intelligent matching prompt function for diagnostic results: Users can customize special vocabulary and corresponding prompt content. When these vocabulary words appear in pathological diagnosis, the system automatically prompts with pop ups;

25. *Intelligent warning function for diagnostic results: if the diagnosis shows "cancer" If it is "tumor" or "positive", the font will turn red as a reminder;

26. Provide fine-grained user permission management to adapt to the permission control of doctors at different levels;

27. Report trace backend recording and traceability query function;

28. LISorHISBidirectional interface, quickly extract patient information and medical advice information, and return reports toLISorHISSystem.

II Cooling camera

(1)Hardware parameters:

1. Physical pixels:2000Wan;

2. Sensor size:13.06x8.76mm；

3. *Type: Refrigeration camera;

4. Color: Black and White;

5. *Target surface:1Inches;

6. Pixel size:2.4µm*2.4µm；

7. *Frame rate:20@5440x3648，48@2736x1824，60@1824x1216；

8. *time of exposure:0.1ms~3600sAccurate and controllable exposure time;

9. *Refrigeration method: Double stage cycle thermoelectric refrigeration system, below ambient temperature 42Below the degree;

10. Depth of Position:8 Bit / 12Bit

11. Data port: High speedUSB3.0Interface, transmission speed up to5Gbits/s.

12. Dustproof structure: processed in a dust-free workshop, with precision dust-proof structure, there will be no dust pollution.

(II)Software control parameters:

1. Support exposure, white balance, color adjustment, black balance, light source frequency, color mode, resolutionROIParameter setting

2. Ultra-FineColor processing engine, achieving excellent color reproduction capability;

3. Highly coupled with the reporting system, supporting batch collection of images for multiple cases;

4. Support collecting parameter combinations and saving, suitable for quick switching between different usage scenarios;

5. Support measurement of line segments, polylines, rectangles, polygons, circles, angles, perimeters, areas, etc;

6. Support multiple types of annotations;

7. Support ruler calibration and automatic addition of rulers;

8. *Support fluorescence image synthesis and processing;

9. *Support automatic image stitchingEDFExpansion functions such as depth of field extension and segmentation counting.

(3)Application Scenario:

1. The camera can be used for capturing low light or fluorescent images, and its main applications include bright field microscopy; Dark field, differential interference(DIC)Microscope; Live cell imaging, cellular or tissue pathology testing, cytology; Low light fluorescence imaging,GFPorRFPAnalysis, fluorescence in situ hybridization(FISH)Fluorescence resonance energy transfer microscope, total internal reflection fluorescence microscope, real-time confocal microscope, failure analysis.