Flow cytometry cell cycle detection service

　　Flow cytometry cell cycle detection serviceThe principle is mainly based on the working principle of flow cytometry. A flow cytometer can place the cells to be tested into a sample tube and introduce them into a flow chamber filled with sheath fluid under gas pressure. Cells are arranged in a single row under the constraint of sheath fluid and sprayed out from the nozzle of the flow chamber, forming a column of cells. The instrument detects the cells arranged in a single row in the flowing liquid one by one, obtains the light scattering and fluorescence indicators of the cells, and then analyzes their volume, internal structure DNA、RNA、 Physical and chemical characteristics such as proteins and antigens. Due to the periodic changes in DNA content within cells during the cell cycle (such as 2C DNA content in G0/G1 phase and 4C DNA content in G2 phase), specific dyes (such as PI dye) can be used to label intracellular DNA, and the relative content of intracellular DNA can be determined by flow cytometry to analyze the percentage of each phase of the cell cycle.

The application scope of flow cytometry cell cycle detection services is very wide, including but not limited to the following aspects:

　　Oncology researchIt can detect the proliferation cycle of tumor cells, which helps to understand the growth rate and malignancy of tumors.

　　Hematological analysisPlays an important role in the diagnosis and treatment monitoring of blood diseases such as leukemia and lymphoma.

　　Immunological fieldCan be used for analysis of lymphocytes and their subsets to understand the function and status of the immune system.

　　Drug development and screeningEvaluate the efficacy and toxicity of drugs by measuring their impact on the cell cycle.

　　Basic Biology ResearchAssist scientists in gaining a deeper understanding of the molecular mechanisms underlying cell cycle regulation.

The steps of flow cytometry cell cycle detection usually include:

　　cell cultureCultivate cells in logarithmic growth phase and perform corresponding treatments according to experimental needs.

　　Cell fixationFix cells using pre cooled ethanol or other fixatives to maintain their morphology and internal structure.

　　cell stainingStain the DNA inside cells with specific dyes (such as PI dyes) for subsequent detection.

　　Flow analysisDetect the stained cells using a flow cytometer, collect data, and analyze it.

analytical method

　　Flow cytometry cell cycle detection serviceThe data analysis mainly relies on professional software such as ModFit. These software can process and analyze the collected data, generating percentage reports for each phase of the cell cycle. By analyzing the report, researchers can understand the proliferation status of the cell population, the distribution of the cell cycle, and the impact of experimental treatments on the cell cycle. In addition, comprehensive analysis can be conducted by combining other biological indicators such as apoptosis rate, cell proliferation index, etc., to provide more comprehensive data support for the experiment.