Exhaled breath condensate collector

The main product of Respiratory Research, Inc. in the United States is Rtube. Rtube is mainly used for the collection and analysis of biomarkers in exhaled breath. Since 2001, Rtube has been used over 200000 times by researchers worldwide.

The main applications of Rtube

The main function of Rtube is to collect exhaled breath condensate (EBC), and EBC detection has the advantages of being non-invasive, easy to operate, and repeatable. The changes in certain components of EBC can reflect the pathophysiological status of the lungs and airways, and are expected to become a means of screening for early lung cancer, monitoring the condition, and assessing therapeutic efficacy in some populations.

EBC is a highly diluted body fluid that can detect volatile substances (such as nitric oxide, carbon monoxide, etc.), non-volatile substances (such as nitrite, ammonia, and vasoactive amines), proteins, peptides, nucleic acids, and some lipid metabolites through special and highly sensitive detection methods. Research reports that thousands of substances can currently be detected in EBC, and new substances are constantly being detected.

At present, the inflammatory markers in EBC, such as *, nitric oxide and its related products (nitrate/nitrite), prostaglandins, leukotrienes, etc., are widely studied both domestically and internationally, which have important application value in the monitoring and treatment of airway inflammation.

Many scholars have found that searching for lung cancer specific tumor markers in EBC and combining multiple markers for lung cancer diagnosis has important application significance for screening, early diagnosis, disease monitoring, and prognosis judgment of lung cancer.

Exhaled breath condensate collectorComposition: Cooling sleeve, insulating cover, pistons, sample tube (Rtube)

Product Features:

Purpose: Used for non-invasive collection of exhaled breath biomarkers for clinical research.

Features:Exhaled breath condensate collectorAdopting a condensation collection method, the freezing sleeve, insulation cover, and piston can be reused. The collection, storage, transfer, and analysis of condensation samples are integrated into the same Rtule, reducing external environmental pollution to the samples. The collection process is simple and reduces the workload of research and experimental personnel.

Case 1: Bronchial asthma is a common disease that poses a threat to human health. Exhaled breath condensate (EBC) detection is a non-invasive method developed in recent years for detecting respiratory inflammation, which has the advantages of simplicity, feasibility, and good reproducibility. It is particularly suitable for patients with severe conditions, as well as the elderly and children (including infants and young children), providing a new means for monitoring the progression and treatment effectiveness of lung diseases.

Case 2: The exhaled breath is considered as the headspace gas in the blood, which is excreted through alveolar exchange and can to some extent reflect the endogenous metabolism of the human body. In recent years, with the continuous development of non-destructive medical diagnosis, exhaled breath analysis has attracted increasing attention due to its non-invasive, painless, convenient, and potential for early diagnosis and large-scale screening of clinical diseases. Research shows that the volatile organic compounds contained in exhaled air are closely related to the metabolic status of human diseases, such as acetone and diabetes, aldehydes and breast cancer, alkanes and oxidative stress levels. Modern analytical methods, such as gas chromatography, mass spectrometry, spectroscopy, sensors, etc., enable qualitative and quantitative analysis of thousands of trace components in exhaled breath, making exhaled breath analysis possible.

Introduction to the collection process:

(1) The subject rinsed their mouth with distilled water three times;

(2) Remove the refrigeration aluminum tube from the -10 ° C refrigerator and assemble it into an exhaled condensate collector;

(3) The subjects underwent oral and calm tidal breathing, with an adult collection time of 7 minutes and a collection volume of 1000 microliters, and a child collection time of 10 minutes and a collection volume of 700 microliters;

(4) During the collection process, the subjects should continuously swallow saliva. If coughing or belching occurs, the interface should be immediately disconnected to prevent contamination of saliva and gastric components;

(5) After collection, remove the collection tube, remove the lower valve device, cover it, and send it to the central laboratory for further operation;

(6) Remove the lid and use a push rod to push up from the bottom of the collection device tube (similar to the action of a syringe), causing the condensate to accumulate at the top;

(7) Use a micropipette to aspirate the condensate into an EP tube and divide it into 200uL tubes.

Technical parameters: