DMEM medium without phenol red and tryptophan

DMEM medium without phenol red and tryptophan
Amino acid deficiency culture medium, customized,2 bottles minimum order, customizable DMEM high sugar, 1640, MEM, DMEM/F12, DMEM low sugar, DMEM sugar free, α - MEM, EMEM, McCoy's 5A, M199 medium, L-15 medium, F12 medium, F-12K medium, William's Medium E, F10 medium, IMDM medium

DMEM does not contain choline and methionine

DMEM high glucose medium contains 4500mg/L D-glucose, 584mg/L L-glutamine, 110mg/L sodium pyruvate, and 3700mg/L sodium bicarbonate. PH value is 7.0-7.4.
Dulbecco's Modified Eagle Medium (DMEM) was developed based on MEM medium. Compared with MEM medium, the content of amino acids increased by 2 times, the content of vitamins increased by 4 times, and non essential amino acids, trace iron ions, and sodium pyruvate were also added. The DMEM medium was initially designed with a glucose content of 1000mg/L (low sugar type), and later developed with a glucose content of 4500mg/L (high sugar type), which has been widely used in the cultivation of various cells.

DMEM high sugar medium is widely used in the culture of fast growing and low adhesion cells, hybridoma myeloma cells, clone cells, DNA transfected transformation cells, various primary virus host cells, single cells and vaccine production, such as the use of CHO cells to express EPO and produce hepatitis B vaccine.

This product contains various components such as amino acids, vitamins, and inorganic salts required for cell culture, but does not contain proteins, lipids, or any growth factors. Therefore, the product needs to be used with serum or serum-free additives.

DMEM medium without phenol red and tryptophan

Shanghai Language PurebiologyNexell specializes in providing full chain solutions in the field of cell culture, using high-purity raw materials, scientifically precise ratios, and batchesstableEfficient and short delivery time, strong capabilitiesbiologyWith technical talents as the core advantage, we provide one-stop customized development and large-scale production services for cell culture media for universities, research institutes, and biopharmaceutical enterprises, fully meeting the personalized training needs of different scenarios such as basic research, drug development, and cell therapy.

Shanghai Language PurebiologyNexell adheres to the principle of prioritizing raw materials and quality, and uses high-purity imported raw materials for all customized culture media. Key indicators such as heavy metals, endotoxins, mycoplasma, and exogenous factors are strictly controlled to prevent impurities from interfering at the source, ensuring stable cell growth and reproducible experimental data. By relying on standardized production systems and full process quality control systems, we achieve high consistency between batches, solve pain points such as large batch differences and state fluctuations in conventional culture media, and make cell culture more worry free and reliable. Relying on the R&D team and years of formula accumulation,Shanghai Language PurebiologyNexell provides customized services with scientific matching and precise optimization. Based on cell characteristics, cultivation methods, and experimental objectives, R&D personnel analyze the nutritional components pH、 Targeted debugging of osmotic pressure, additives, etc., balancing proliferation efficiency, cell activity, and experimental stability, making each customized culture medium more suitable for practical application scenarios. At the same time, the company has the ability to produce on a large scale and respond quickly, significantly shortening the customization cycle, achieving short lead times and fast delivery, and efficiently supporting scientific research and project progress.

Shanghai Language PurebiologyNexell has a research and development platform, standardized production workshop, and professional quality control system, with adequate technical support; The core team is composed of talents in fields such as cell biology and bioengineering, with solid theoretical foundation and rich engineering experience. They can quickly respond to complex customized needs and provide full process technical support for formula development, small-scale optimization, pilot scale amplification, and mass production.

Customized types of commonly used cell culture media（Nexell's mainstream customized product category

DMEM medium: High sugar/low sugar optional, suitable for most adherent cells such as tumor cells, 293, CHO, etc., supporting conventional culture and transfection experiments.

RPMI 1640 medium: suitable for lymphoma, leukemia cells, hybridoma cells, and most suspension cell lines, with strong universality and wide applicability.

DMEM/F12 medium: Combining DMEM high nutrient and F12 rich components, suitable for stem cell, primary cell, and sensitive cell culture.

F12K medium: specially designed for insect cells, primary cells, and demanding cell lines, with balanced nutrition and support for efficient proliferation.

simultaneously provideMEM、α-MEM、IMDM、F12P medium, F10 medium, William's Medium E, MCDB131, IMDM medium, L-15 medium, M199 medium, McCoy's 5A medium, EMEM mediumSerum free medium, phenol red free medium, amino acid deficient mediumlosetypeBiotin deficiency, inorganic salt deficiencyCustomization of all types of culture media, including serum, additives, buffer systems, and packaging specifications, can be adjusted as needed to meet personalized and specialized cultivation needs.

choiceShanghai Language PurebiologyNexell customized culture medium refers to selecting high-quality, stable, and efficient cell culture guarantees, empowering scientific research with professionalism, assisting in the transformation of results with quality, and providing reliable core support for cell experiments and biological preparation.