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DMEM/F12 medium does not contain D-calcium pantothenate News
NegotiableUpdate on 05/06
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Overview
DMEM/F12 medium does not contain D-calcium pantothenate. News: DMEM/F12 is a composite medium of DMEM and F12 mixed in a 1:1 ratio. It is nutritionally comprehensive, rich in trace elements, and suitable for low serum and serum-free cultures. It is widely used for primary cell, stem cell, and various adherent cell cultures and is a commonly used basic culture medium in biological experiments.
Product Details
DMEM/F12 medium does not contain D-calcium pantothenate News
Shanghai Yuchun Biotechnology Co., Ltd. is a technology enterprise jointly founded by personnel with backgrounds in life sciences and biotechnology. Main business of the company:Cell lines, primary cells, specialized culture media for primary endothelium, epithelium, fibroblasts, chondrocytes, etc. (can replace imported brands such assciencellItem No.1001）Basic cell culture medium and customized amino acid deficiency culture medium (e.g.:DMEMHigh sugarRPIM1640TheMEMTheDMEM/F12Microbial culture media and strains, imported fetal bovine serum, domestic fetal bovine serum, newborn bovine serum, calf serum, horse serum, exosome free fetal bovine serum, dialysis fetal bovine serum, etc., can be customized according to needs. Biochemical reagent kit,elisaReagent kits, animal blood products: animal plasma, animal red blood cells, animal whole blood, animal white blood cells, etc., sensory, various biochemical reagents and consumables. Provide relevant technical support and services for universities, food, medicine, biological research and other fields.
Shanghai Yuchun Biotechnology Co., Ltd., customized cell basic culture medium advantages:
Flexibility: Based on experimental requirements and the characteristics of specific cell lines, the composition and ratio of the culture medium can be flexibly adjusted to provide efficient and favorable cultivation conditions.
Efficiency: Formula optimization can quickly promote cell proliferation and differentiation, shorten cultivation time.
Safety: Strictly control the concentration of nutrients and metabolites to avoid toxicity to cells.
High consistency: By optimizing the formula and standardizing the production process, ensure consistent cultivation conditions between batches.
Targeted: Design the composition of the culture medium according to the experimental objectives to avoid unnecessary interference from components.
DMEM/F12 medium does not contain D-calcium pantothenate News
Customized types of commonly used cell culture media（NexellMainstream Customized Category
DMEMCulture medium: high sugar/Low sugar options, suitable for tumor cells293TheCHOWait for the majority of adherent cells to support conventional culture and transfection experiments.
RPMI 1640Culture medium: Suitable for lymphoma, leukemia cells, hybridoma cells, and most suspension cell lines, with strong universality and wide applicability.
DMEM/F12Culture medium: combined withDMEMHigh nutrition andF12Rich components, suitable for stem cell, primary cell, and sensitive cell culture.
F12KCulture medium: specifically designed for insect cells, primary cells, anddemandingCell line design, balanced nutrition, supports efficient proliferation.
simultaneously provideMEMand α-MEMTheIMDMTheF12Culture mediumF10Culture mediumWilliam’s Medium ETheMCDB131TheIMDMCulture mediumL-15Culture mediumM199Culture mediumMcCoy’s 5ACulture mediumEMEMCustomization of various types of culture media, including culture media, serum-free culture media, phenol red free culture media, amino acid deficient culture media, biotin deficient culture media, and inorganic salt deficient culture media. Serum, additives, buffer systems, and packaging specifications can be adjusted as needed to meet personalized and specialized culture needs.
Choose Shanghai Language Pure Biology（Nexell）Customized culture medium means selecting high-quality, stable, and efficient cell culture guarantees, empowering scientific research with professionalism, assisting in the transformation of results with quality, and providing reliable core support for cell experiments and biological preparation.
Common problems with cell culture media
Common problems in cultivating basic body
1. pHAbnormal (slightly acidic)/Slightly alkaline)
Reason:CO₂ Incorrect concentration, bottle cap too tight, contamination, high temperature, during preparationpHnot allowed
Solution: CalibrationCO₂ Incubator; Don't twist the breathable cover tightly; Freshly prepared; Store in the dark
2. Sedimentation, turbidity, and discoloration occur
Reason: Repeated freeze-thaw cycles, calcium ions/Phosphate reaction, pollution, expiration, high temperature
Solution: Avoid multiple freeze-thaw cycles; Packaging and use;4℃ storage; Discard directly if there is sediment
3. Incorrect osmotic pressure, cell shrinkage or rupture
Reason: Inaccurate salt concentration, wrong serum addition, incorrect dilution
Solution: strictly follow the instructions for preparation; Using the same batch of water and reagents
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| 1 | Language Pure (NEXELL) | DMEM/F12Does not contain glycine | 500ml | YC-2117 |
| 2 | Language Pure (NEXELL) | DMEM/F12Does not contain L-alanine | 500ml | YC-2118 |
| 3 | Language Pure (NEXELL) | DMEM/F12Does not contain L-arginine hydrochloride | 500ml | YC-2119 |
| 4 | Language Pure (NEXELL) | DMEM/F12Free of L-asparagine monohydrate | 500ml | YC-2120 |
| 5 | Language Pure (NEXELL) | DMEM/F12Does not contain L-aspartic acid | 500ml | YC-2121 |
| 6 | Language Pure (NEXELL) | DMEM/F12Free of L-cysteine hydrochloride monohydrate | 500ml | YC-2122 |
| 7 | Language Pure (NEXELL) | DMEM/F12Does not contain L-glutamic acid | 500ml | YC-2124 |
| 8 | Language Pure (NEXELL) | DMEM/F12Does not contain L-glutamine | 500ml | YC-2065 |
| 9 | Language Pure (NEXELL) | DMEM/F12Free of L-histidine hydrochloride monohydrate | 500ml | YC-2126 |
| 10 | Language Pure (NEXELL) | DMEM/F12Does not contain L-isoleucine | 500ml | YC-2127 |
| 11 | Language Pure (NEXELL) | DMEM/F12Does not contain L-leucine | 500ml | YC-2128 |
| 12 | Language Pure (NEXELL) | DMEM/F12Does not contain L-lysine hydrochloride | 500ml | YC-2129 |
| 13 | Language Pure (NEXELL) | DMEM/F12Does not contain L-methionine | 500ml | YC-2130 |
| 14 | Language Pure (NEXELL) | DMEM/F12Does not contain L-phenylalanine | 500ml | YC-2131 |
| 15 | Language Pure (NEXELL) | DMEM/F12Does not contain L-proline | 500ml | YC-2132 |
| 16 | Language Pure (NEXELL) | DMEM/F12Does not contain L-serine | 500ml | YC-2133 |
| 17 | Language Pure (NEXELL) | DMEM/F12Does not contain L-threonine | 500ml | YC-2134 |
| 18 | Language Pure (NEXELL) | DMEM/F12Does not contain L-tryptophan | 500ml | YC-2083 |
| 19 | Language Pure (NEXELL) | DMEM/F12Does not contain L-tyrosine disodium salt dihydrate | 500ml | YC-2135 |
| 20 | Language Pure (NEXELL) | DMEM/F12Does not contain L-valine | 500ml | YC-2136 |
| 21 | Language Pure (NEXELL) | DMEM/F12Does not contain biotin | 500ml | YC-2137 |
| 22 | Language Pure (NEXELL) | DMEM/F12Does not contain choline chloride | 500ml | YC-2138 |
| 23 | Language Pure (NEXELL) | DMEM/F12Does not contain D-calcium pantothenate | 500ml | YC-2139 |
| 24 | Language Pure (NEXELL) | DMEM/F12Does not contain folic acid | 500ml | YC-2140 |
| 25 | Language Pure (NEXELL) | DMEM/F12No niacinamide | 500ml | YC-2141 |
| 26 | Language Pure (NEXELL) | DMEM/F12Does not contain pyridoxine hydrochloride | 500ml | YC-2142 |
| 27 | Language Pure (NEXELL) | DMEM/F12Does not contain riboflavin | 500ml | YC-2143 |
| 28 | Language Pure (NEXELL) | DMEM/F12Does not contain thiamine hydrochloride | 500ml | YC-2144 |
| 29 | Language Pure (NEXELL) | DMEM/F12Does not contain vitamin B12 | 500ml | YC-2145 |
| 30 | Language Pure (NEXELL) | DMEM/F12Does not contain i-inositol | 500ml | YC-2146 |
| 31 | Language Pure (NEXELL) | DMEM/F12Does not contain anhydrous calcium chloride | 500ml | YC-2147 |
| 32 | Language Pure (NEXELL) | DMEM/F12Free of copper sulfate pentahydrate | 500ml | YC-2148 |
| 33 | Language Pure (NEXELL) | DMEM/F12Does not contain iron nitrate nonahydrate | 500ml | YC-2149 |
| 34 | Language Pure (NEXELL) | DMEM/F12Does not contain ferrous sulfate heptahydrate | 500ml | YC-2150 |
| 35 | Language Pure (NEXELL) | DMEM/F12Does not contain anhydrous magnesium chloride | 500ml | YC-2151 |
| 36 | Language Pure (NEXELL) | DMEM/F12Does not contain anhydrous magnesium sulfate | 500ml | YC-2152 |
| 37 | Language Pure (NEXELL) | DMEM/F12Does not contain sodium chloride | 500ml | YC-2154 |
| 38 | Language Pure (NEXELL) | DMEM/F12Free of anhydrous disodium hydrogen phosphate | 500ml | YC-2155 |
| 39 | Language Pure (NEXELL) | DMEM/F12Does not contain sodium dihydrogen phosphate monohydrate | 500ml | YC-2156 |
| 40 | Language Pure (NEXELL) | DMEM/F12Does not contain zinc sulfate heptahydrate | 500ml | YC-2157 |
| 41 | Language Pure (NEXELL) | DMEM/F12Does not contain glucose (HEPES) | 500ml | YC-2074 |
| 42 | Language Pure (NEXELL) | DMEM/F12Does not contain sodium hypoxanthine | 500ml | YC-2158 |
| 43 | Language Pure (NEXELL) | DMEM/F12Does not contain linoleic acid | 500ml | YC-2159 |
| 44 | Language Pure (NEXELL) | DMEM/F12不含硫辛酸 | 500ml | YC-2160 |
| 45 | Language Pure (NEXELL) | DMEM/F12Free of phenol red | 500ml | YC-2189 |
| 46 | Language Pure (NEXELL) | DMEM/F12Does not contain putrescine hydrochloride | 500ml | YC-2161 |
| 47 | Language Pure (NEXELL) | DMEM/F12Does not contain sodium pyruvate | 500ml | YC-2188 |
| 48 | Language Pure (NEXELL) | DMEM/F12Does not contain thymidine | 500ml | YC-2162 |
| 49 | Language Pure (NEXELL) | DMEM/F12Excluding HEPES | 500ml | YC-2059 |
| 50 | Language Pure (NEXELL) | DMEM/F12 medium (HEPES) | 500ml | YC-2061 |
| 51 | Language Pure (NEXELL) | DMEM /F12Sugar free (HEPES) | 500ml | YC-2074 |
| 52 | Language Pure (NEXELL) | DMEM/F12 Does not contain putrescine hydrochloride and arginine hydrochloride (HEPES) | 500ml | YC-2178 |
| 53 | Language Pure (NEXELL) | DMEM/F12 Does not contain biotin (including GlutaMAX) (HEPES) | 500ml | YC-2177 |
| 54 | Language Pure (NEXELL) | DMEM/F12 Does not contain choline chloride Methionine. Hepes | 500ml | YC-2169 |
| 55 | Language Pure (NEXELL) | DMEM/F12Contains HPES without branched chain amino acids (leucine, valine, and isoleucine) | 500ml | DZ0011 |
| 56 | Language Pure (NEXELL) | DMEM/F12Does not contain branched chain amino acids (leucine, valine, and isoleucine) | 500ml | DZ0016 |
| 57 | Language Pure (NEXELL) | DMEM/F12Containing HEPES (2450mg/L sodium bicarbonate) | 500ml | YC-2081 |
| 58 | Language Pure (NEXELL) | DMEM/F12 Does not contain amino acids (HEPES) | 500ml | YC-2105 |
| 59 | Language Pure (NEXELL) | DMEM/F12 medium2X (including HEPES, excluding phenol red) | 500ml | YC-2186 |
| 60 | Language Pure (NEXELL) | DMEM/F12 medium2X (without HEPES, without phenol red) | 500ml | YC-2185 |
| 61 | Language Pure (NEXELL) | DMEM /F12(Excluding HEPES and containing dual antibodies) | 500ml | YC-2060S |
| 62 | Language Pure (NEXELL) | DMEM /F12(2X)HEPES | 500ml | YC-2116 |
| 63 | Language Pure (NEXELL) | DMEM /F12Does not contain calcium ions (hepes) | 500ml | YC-2076 |
| 64 | Language Pure (NEXELL) | DMEM /F12(including HEPES and bispecific antibodies) | 500ml | YC-2062S |
| 65 | Language Pure (NEXELL) | DMEM /F12(Excluding HEPES and phenol red) | 500ml | YC-2071 |
| 66 | Language Pure (NEXELL) | DMEM /F12(including HEPES, excluding phenol red) | 500ml | YC-2063 |
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