D-Squame pressure gauge D500 Cuderm

summary

The D-Squame pressure gauge D500 is researched and produced by Cuderm in the United States. It has a barrel like appearance and a built-in spring pressure device.

When sampling the skin, the D-Squame pressure gauge can be placed on the skin to provide uniform external pressure to the D-Squame tape adhered to the surface of the skin, achieving uniform collection of skin stratum corneum cells, and then observing and analyzing the skin condition and pathogenic molecules.

1. Introduction to tape peeling method

The stratum corneum, as the outermost layer of the epidermis, is composed of highly specialized non nucleated keratinocytes and lipids filled between cells. Its main function is to prevent the loss of epidermal water and the invasion of exogenous substances. This type of cell appears uniformly reddish on HE stained sections, with unclear cell contours and loose connections at the surface, tending to shed and form scales. The tape peeling method, as a physical stimulation model, has the advantages of short evaluation cycle and objective quantitative evaluation, and is suitable for in vivo and in vitro skin models as well as research on human and animal skin.

2. Operation steps of pressure gauge D500

Firstly, stick the D-Squame D100 tape onto the skin and place the pressure gauge D500 on the tape.

Then, press the top piston with your thumb until the bottom of the piston is tightly attached to the tape.

Finally, apply pressure again until the thumb feels the pressure, indicating that the pressure gauge has achieved a pressure of 225 grams per square centimeter. Press for 5 seconds and follow the instructions to remove the tape.

3. Post treatment and analysis of tape peeling materials

Firstly, morphological or biophysical markers (such as keratinocyte hardness or topological structure) can be directly measured on adhesive tape.

Secondly, molecular markers must be extracted from the tape before analysis and determination. The type of solvent used for extraction, the extraction technique (oscillation, vortex or ultrasound), and the amount of tape required to obtain a sufficiently large sample all depend on the specific biomarker. Immune markers and antimicrobial peptides can be extracted using various lysates with added protease inhibitors or surfactants. High lipophilic biomarkers, such as ceramides or free fatty acids, typically require multi-stage extraction using organic solvents of different polarities. Gene expression profiling analysis usually uses RNA isolation and lysis buffer.

4. Application

Firstly, measure biomarkers in various skin pathologies; The research on biomarkers mainly focuses on monitoring treatment efficacy and typing, as well as RNA quantitative analysis in the molecular field.

Secondly, evaluate the skin absorption of the drug.

Thirdly, induce standardized skin barrier damage.

In summary, the D-Squame D500 pressure gauge can provide uniform pressure for the collection of stratum corneum samples, and when combined with the D-Squame D100 tape and protein measurement equipment, the D-Squame D501 makes the sample collection process more standardized, and the collection results more stable and accurate.