D-Squame D100 tape Cuderm

summary

D-Squame D100Tape from the United StatesCudermResearch and production involves using adhesive tape coated with a sticky material, which is applied to the skin. The adhesive on the tape can evenly and thoroughly remove keratinocytes, and efficiently and quantitatively extract target molecules from the tape, thereby observing physiological and pathological changes in the stratum corneum. This technology is widely used for kinetic studies of drugs and cosmetics, as well as for observing the penetration depth of active substances.

1Introduction to tape peeling method

The stratum corneum, as the outermost layer of the epidermis, is composed of highly specialized non nucleated keratinocytes and lipids filled between cells. Its main function is to prevent the loss of epidermal water and the invasion of exogenous substances. This highly specific non nuclear keratinocyte isHEOn the stained section, it appears uniformly reddish in color, with unclear cell contours and loose cell connections on the surface, tending to shed and known as scales. The tape peeling method, as a physical stimulation model, is applied to in vivo and in vitro skin models as well as human and animal skin due to its advantages of short evaluation cycle and objective quantitative evaluation.

2Operating steps of tape peeling method

First,D-Squame D100Stick the tape on the skin to avoid folding. When peeling from the same skin area multiple times, the position of the tape can be marked with a pen or template.

Again, useD-Squame D500Pressure gauge applies pressure5-15After a second, quickly tear off the tape with tweezers. The use of a pressure gauge is to increase the adhesion between epidermal cells and adhesive tape and collect them uniformly. If repeated adhesion to the same skin area is required, in order to improve the uniformity of stratum corneum peeling, it should be continuously and alternately peeled offD-Squame D100tape

Finally, place each adhesive tape facing inward into the small bottle, or place it inD-SquameD120On the cardboard.

3，D-Squame D100Post treatment and analysis of tape stripping material

Firstly, morphological or biophysical markers (such as keratinocyte hardness or topological structure) can be directly measured on adhesive tape.

Secondly, molecular markers must be extracted from the tape before analysis and determination. The type of solvent used for extraction, the extraction technique (oscillation, vortex or ultrasound), and the amount of tape required to obtain a sufficiently large sample all depend on the specific biomarker. Immune markers and antimicrobial peptides can be extracted using various lysates with added protease inhibitors or surfactants. High lipophilic biomarkers, such as ceramides or free fatty acids, typically require multi-stage extraction using organic solvents of different polarities. Gene expression profiling analysis, commonly usedRNASeparate the lysis buffer.

4, Application

Firstly, measure biomarkers in various skin pathologies; The research on biomarkers mainly focuses on monitoring treatment efficacy and typing, as well as the molecular fieldRNAQuantitative analysis.

Secondly, evaluate the skin absorption of the drug.

Thirdly, induce standardized skin barrier damage.

In conclusion，D-Squame D100Adhesive tape can not only uniformly and efficiently collect samples of the stratum corneum, but also has low allergic reactions after collection. at the same timeD-Squame D100Tape can be compatible with protein measurement equipment-D-Squame D501It is also compatibleD-Squame D500Pressure gauges make the sample collection process more standardized, resulting in more stable and accurate collection results.