CytoFLEX Nano Nanoflow Analyzer

CytoFLEX Nano Nanoflow Analyzer

As a flow cytometer specially designed for nanoparticle analysis, CytoFLEX nano breaks through the limitations of traditional flow cytometry and provides researchers with a solutionProvide high sensitivity、The results are reproducible and the operation is simple and flexibleThe solution is to accelerate the research process.

1Product Features

Clearly distinguish small particle samples

The CytoFLEX nano flow cytometer is capable of detecting sample populations (40nm-1 µ m) that traditional flow cytometry cannot detect

Suitable for the research of various nanoscale small particles such as exosomes, viral particles, liposomes, and migratory bodies.

New ideas for experimental design of opening multiple lateral scattering channels

5 lateral scattering channels

By using the SSC scattering light ratio of different channels, different subgroups of small particles can be separated and identified without the need for dyes

True multi-color small particle detection

Equipped with four wavelength lasers: 405nm, 488nm, 561nm, and 638nm

High configuration with 6 fluorescent channels

• Covering the main dyes used in small particle research

high sensitivity+High resolution ensures accurate detection results

CytoFLEX nano can not only detect small particles, but also study the low-density antigens on their surfaces

• Detection in various fluorescence channels of CytoFLEX nano500nmEight peak microspheres have better performance

When characterizing particles of various sizes, it is possible to clearly distinguish groups with a size difference of at least 10nm

Strict quality control brings trustworthy results

4 new automated process optimization instrument quality control management, all processes are automated or semi automated

Baseline monitoring: Check if it is necessary to clean the instrument or sample buffer to ensure that the contamination rate is less than 1%

Sensitivity monitoring: Match the results of 500nm eight peak microspheres with standard data to evaluate the fluorescence sensitivity of the instrument.

• Avoid common flow problems such as blockages and bubbles, and simplify instrument maintenance steps

2 Scope of application

Extracellular vesicle detection

Derived from human cell linesHEK293TMembrane surface expressionGFPPurified extracellular vesicles(50-150nm)

Using traditional flow cytometry, the characterization of extracellular vesicles is incomplete because we can only see things as low as 100 nanometers. Using CytoFLEX nano nanoflow cytometry, we can observe GFP staining in the extracellular vesicles, indicating that we can more comprehensively characterize the extracellular vesicles.

Other applications: virus particle detection, liposome detection, etc.