Creatinine content detection kit

Creatinine content detection kit $r $n Creatinine amide hydrolase can catalyze creatinine to produce creatine. Creatine is catalyzed by creatine enzyme to produce creatine and urea. Creatine oxidase catalyzes the oxidation of creatine to produce glycine, formaldehyde, and *. Peroxidase further catalyzes the oxidation of 4-aminoantipyrin and 2,4-DCP to produce purple red quinone compounds. The product has a characteristic absorption peak at 546 nm, and the creatinine content can be quantitatively detected by changes in absorbance.

Boxbio®Creatinine (CRE) Content Assay Kit

Creatinine (CRE) content detection kit

creatinineCRE is a compound produced during muscle metabolism, formed by the spontaneous and irreversible conversion of phosphocreatine. Blood creatinine comes from both exogenous and endogenous sources. Exogenous creatinine is mainly produced by the metabolism of meat in the body, while endogenous creatinine is a product of muscle tissue metabolism. The generation and excretion of creatinine in the body are closely related to kidney function, and an increase in creatinine levels usually indicates impaired or failed kidney function. The measurement of creatinine levels plays an important role in evaluating kidney function and monitoring kidney diseases.

Creatinine amide hydrolase can catalyze the production of creatine from creatinine. Creatine is then converted into creatine and urea under the catalysis of creatine kinase. Creatine oxidase catalyzes the oxidation of creatine to produce glycine, formaldehyde, and *. Peroxidase further catalyzes the oxidation of 4-aminoantipyrin and 2,4-DCP to produce purple red quinone compounds. The product has a characteristic absorption peak at 546 nm, and the content of creatinine can be quantitatively detected by changes in absorbance.

Creatinine content detection kitInstruments and reagents required during the measurement process：Visible spectrophotometer/Enzyme linked immunosorbent assay (ELISA) reader, micro glass colorimetric dish (optical path 10 mm)）/96Orifice plate, mortar/Homogenizer, adjustable pipette, desktop centrifuge, constant temperature water bath/Incubator and distilled water.

Sample processing (sample size and proportion can be adjusted appropriately based on pre experimental results)

① Organization: According to organizational quality (g)）Volume of extraction solution (mL)）For 1:（5-10）The ratio (recommended to weigh 0.1 g)Organize, add 1 mLExtract the sample, homogenize in an ice bath, 12000 g at 4 ℃Centrifuge for 10 minutesTake the supernatant and place it on ice for testing.

②Serum (slurry) and other liquid samples: direct detection orDilute appropriately before testing, and if turbid, centrifuge appropriately to obtain the supernatant for measurement.

Creatinine content detection kitPrecautions during the measurement process

① If ∆ AMeasurement greater than 0.4It is recommended to dilute the test sample with distilled water before conducting the measurement; If Δ AMeasurement less than 0.01It is recommended to increase the sample size appropriately before conducting the measurement, and make corresponding modifications when calculating;

②To ensure accurate results and avoid reagent loss, please carefully read the instructions before testing (based on the actual received instructions), confirm whether the reagent storage and preparation are sufficient, whether the operating steps are clear, and be sure to take 2-3For samples with significant expected differences, please contact the staff promptly if there are any issues during the pre testing process.

Pre purchase notice:

A. Determine the type, specifications, and quantity of reagent kits based on the indicators to be tested;

B. Confirm whether the testing equipment and conditions match;

C. Confirm whether the delivery time and receipt time meet the requirements;

★Please contact Boxbio customer service or sales specialist promptly for any questions before purchasing

Notice of Receipt:

A. Please ensure that the phone is open so that users can track the delivery status and complete the receipt based on the tracking number;

B. Please carefully inspect the item number, packaging, quantity, and appearance of the goods upon receipt (for any damage, leakage, contamination, etc.). If there are any issues with the product delivery, please contact Boxbio within 15 days;

C. After receiving the goods, please store them separately according to the temperature indicated on the product label, and use and dispose of the relevant waste correctly according to the conditions described in the instructions;

D. For product quality issues, please provide a written explanation and detailed test results for reference within 3 months from the date of receipt of the product. If you do not raise any objections to the product quality within the above period, it will be deemed that you acknowledge the product as qualified;

E. The expiration date of the BOXBIO reagent kit is 3-6 months. If the product is used unopened after the expiration date, we can exchange the product for you free of charge;

F. The express delivery costs incurred for returns and exchanges due to product quality issues will be borne by our company.

Product Customization

Boxbio provides customized services for large packaging products:

Regarding the price:The salesperson will communicate with you, inquire about the customized reagent kit you need, and negotiate the required specifications based on the sample size to be tested, and then provide you with a quotation.

Regarding packaging:Production and warehouse delivery will match the corresponding packaging according to the specifications of the reagent kit you have customized. As it is a customized product, the packaging provided may not be of regular specifications. Please be aware.

Regarding delivery time:The delivery time for customized products will be calculated based on the type and specifications of the reagent kit you have customized, and will be communicated to you by sales personnel.

Research Assistance Fund

Boxbio is committed to making scientific research easier. In order to encourage and support academic research by researchers, we have launched the "Research Assistance Fund" reward program! Any SCI article published that indicates that the product is provided by Beijing Boxbio Science&Technology Co., Ltd. can apply for the corresponding research assistance fund!

According to the journal impact factor, the reward rules are as follows:

⑴Journal impact factor: 0<IF<5, research assistance fund amount is 300 yuan;

⑵Journal impact factor: 5 ≤ IF ＜ 10, research assistance fund amount is 800 yuan;

⑶Journal impact factor: 10 ≤ IF ＜ 15, research assistance fund amount is 1500 yuan;

⑷Journal impact factor: 15 ≤ IF ＜ 20, research assistance fund amount is 2500 yuan;

⑸Journal impact factor: 20 ≤ IF, research assistance fund amount is 3500 yuan.

*Non SCI articles can apply for a beautiful gift and receive corresponding points.

Application Instructions:

⑴The article must clearly indicate that the corresponding product is sourced from Beijing Box Biotechnology Co., Ltd. or Beijing Boxbio Science ＆ Technology Co.,Ltd.；

⑵The reward is only available to the first author of the article, and co authors can only apply for one reward;

⑶The same article can only apply for research funding once;

⑷If the products cited in the literature are trial equipment, they will not participate in this activity;

⑸The interpretation rights of this event belong to Beijing Box Biotechnology Co., Ltd.

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