Comixsil HAC liquid chromatography column separation mechanism: ionizable acidic and basic groups are incorporated into the hydrophilic chain, which has anion/cation exclusion/exchange function, can form hydrogen bonds and exhibit HILIC (hydrophilic interaction chromatography) effect, effectively retaining strong hydrophilic compounds while achieving accurate analysis of anions and cations.
Comixsil HAC liquid chromatography columnSpecification ModelThere are two options for particle size: 3 μ m and 5 μ m. There are various combinations of column length and inner diameter, such as 2.1x50mm, 2.1x100mm, 3.0x150mm, 4.6x250mm, etc. The corresponding product numbers are FMB-HAC3-BUNU, FMD-HAC3-BUNU, FMF-HAC5-YONU, FMG-HAC5-YONU, etc.
Comixsil HAC liquid chromatography columnapplication fieldCan be used to separate aminoglycosides from streptomycin and dihydrostreptomycin, analyze 8 aminoglycoside substances, separate 11 nucleic acid bases and nucleosides, etc. It can also be used to analyze metal ions, such as simultaneous analysis of zinc, calcium, copper and other metal ions and corresponding anions, separation of calcium and zinc ions, etc. When analyzing nicotine, compared to C18 and Hilic columns, it can avoid weak retention, tailing, and reproducibility issues under acidic conditions.
Comixsil HAC is particularly suitable for analyzing the following types of substances:
Strong hydrophilic compoundDue to its hydrophilic interaction chromatography (HILIC) function, it can effectively retain compounds with strong polarity and hydrophilicity, solving the problem of weak retention of such substances on traditional reverse phase chromatography columns.
Yin Yang ionic substancesDue to the incorporation of ionizable acidic and basic groups in the chromatographic column, it has the functions of anion/cation exclusion and exchange, and can accurately analyze various anions and cations, such as metal ions such as zinc, calcium, copper, and corresponding anions.
Specific biological small moleculesSuch as aminoglycoside antibiotics, nucleic acid bases and nucleosides, can achieve good separation effects.
Substances such as nicotine that are prone to retention and peak shape issuesWhen analyzing nicotine, compared to C18 columns and ordinary Hilic columns, it can avoid weak retention, tailing, and poor reproducibility under acidic conditions, and performs better.
Sample propertiesIf the analyzed sample matrix is complex (such as containing a large amount of impurities, highly corrosive substances, or proteins in biological samples), it will accelerate the contamination or loss of chromatographic column packing and shorten the service life; And clean samples can prolong the column efficiency retention time.
Terms of UseIncluding the pH value of the mobile phase (extreme acid-base conditions may damage the stationary phase), flow rate (excessive flow rate will increase column pressure and accelerate packing wear), column temperature (excessive temperature may affect the stability of the stationary phase), etc. Reasonable control of these parameters can prolong the service life.
maintenanceRegular column flushing (such as rinsing residual samples with appropriate solvents after analysis), avoiding dry columns, and proper storage (such as short-term storage with mobile phase and long-term storage with pure organic solvents) can effectively extend the service life of chromatography columns.
Under normal use and proper maintenance, the service life of such chromatography columns can typically reach hundreds to thousands of injections. The specific lifespan needs to be determined based on the actual usage scenario. If there is an abnormal increase in column pressure, deterioration of peak shape (such as tailing or splitting), or a decrease in separation, it may mean that the chromatographic column needs to be replaced.
| Item number |
Description |
Specifications |
| FMB-HAC5-BONU |
Comixsil HAC 5u |
2.1x50 |
| FMD-HAC5-BONU |
Comixsil HAC 5u |
2.1x100 |
| FMF-HAC5-BONU |
Comixsil HAC 5u |
2.1x150 |
| FMG-HAC5-BONU |
Comixsil HAC 5u |
2.1x250 |
| FMB-HAC5-YONU |
Comixsil HAC 5u |
3.0x50 |
| FMD-HAC5-YONU |
Comixsil HAC 5u |
3.0x100 |
| FMF-HAC5-YONU |
Comixsil HAC 5u |
3.0x150 |
| FMG-HAC5-YONU |
Comixsil HAC 5u |
3.0x250 |
| FMB-HAC5-EONU |
Comixsil HAC 5u |
4.6x50 |
| FMD-HAC5-EONU |
Comixsil HAC 5u |
4.6x100 |
| FMF-HAC5-EONU |
Comixsil HAC 5u |
4.6x150 |
| FMG-HAC5-EONU |
Comixsil HAC 5u |
4.6x250 |
| FMB-HAC3-BONU |
Comixsil HAC 3u |
2.1x50 |
| FMD-HAC3-BONU |
Comixsil HAC 3u |
2.1x100 |
| FMF-HAC3-BONU |
Comixsil HAC 3u |
2.1x150 |
| FMG-HAC3-BONU |
Comixsil HAC 3u |
2.1x250 |
| FMB-HAC3-YONU |
Comixsil HAC 3u |
3.0x50 |
| FMD-HAC3-YONU |
Comixsil HAC 3u |
3.0x100 |
| FMF-HAC3-YONU |
Comixsil HAC 3u |
3.0x150 |
| FMG-HAC3-YONU |
Comixsil HAC 3u |
3.0x250 |
| FMB-HAC3-EONU |
Comixsil HAC 3u |
4.6x50 |
| FMD-HAC3-EONU |
Comixsil HAC 3u |
4.6x100 |
| FMF-HAC3-EONU |
Comixsil HAC 3u |
4.6x150 |
| FMG-HAC3-EONU |
Comixsil HAC 3u |
4.6x250 |
| FMF-HAC5-NONU |
Comixsil HAC 5u |
10x150 |
| FMG-HAC5-NONU |
Comixsil HAC 5u |
10x250 |
