ClearFirst-4500 protein purification system

ClearFirst-4500 instrument features:

1Using high voltageconstant currentThe pump forms a high-precision gradient curve to avoid the generation of bubbles, which has high practical value;

2Using high-precision constant flow pumps, with small pulsation, stable baseline, accurate flow output, and performance far superior to peristaltic pump systems;

3Traffic volume01200mL/min，Greatly improve work efficiency；

4Medium pressure chromatography system,The basic pressure pipeline of the system isbiologically inertMaterial, good pressure resistance and biocompatibility;

5Can form high-quality gradient curves, with performance far superior to gradient cup connected systems;

6By using a four wavelength detector, it is possible to190-850 nmChoose any four wavelengths for simultaneous detection;

7Using a grating monochromator, the wavelength accuracy is better than0.2 nm，The detection sensitivity is better than that of diode array products;

8The circulation pool can adopt different optical paths to meet different purification requirements;

9A specialized conductivity detector with a large range and high precision, with the same principle and structure as imported products;

10、selectmatchpHThe detector has temperature correction function and its accuracy is better than imported products;

11Support the selection of pre column and post column pressure sensors to monitor system pressure changes and effectively protect the chromatographic column;

12Support optional selectionFully automatic high-pressure sampling valve，Using a multi valve injection method ensures accurate injection and prevents air from entering the system;

13ConfigurationMulti way valve buffer expansion component, supporting4aBuffer solution inlet channel，Develop a more flexible buffer selection system;

14、Configure a large volume injection system and injection pump, with a maximum flow rate of up to600mL/min，Used for large volume injection;

15Sample selection valve support8A sample loading channel provides a more diverse sample selection system;

16Configure a bypass system for rapid replacement of buffer solution and pressure regulation;

17Configure a reverse flushing system to control the flow direction of the liquid path, which can be used for online flushing of chromatography columns;

18、UseLarge volume collection valve, with12An export that can collect samples according to customer needs；

19Supporting the optional fully automatic fraction collector eliminates pipe jumping, disorder, and overflow phenomena, and has high stability;

20, DedicatedClearFirst-4500Protein purification workstation,The instrument can be fully controlled by a computer, greatly reducing the difficulty of use;

21Equipped with a method database, different purification methods can be pre-set and called at any time, and automatic operations can be completed with just one click;

22Can perform instrument self inspection, user management, and sample management;

23The workstation has a fully Chinese interface, which is convenient to use and in line with the usage habits of Chinese people;

24、modularDesignFlexible and convenient operation；

25This instrument supports fully automatic sampler and fully unmanned operation.

26The hardware configuration and software functions of this instrument are comprehensively superior toAKTA pilotPurification system,Function*，Has a high cost performance ratio，It is an ideal choice for protein purification laboratories;

ClearFirst-4500 instrument composition:

1、System pumpComponents (including high-pressure constant current pump)ApumpTheBPumpBuffer selection valve*2）；

2、Sample pump components (including sample pump, sample selection valve);

3、Detector componentA(including full wavelength ultraviolet detector, flow cell, digital to analog converter);

4、Detector componentB(includingConductivity detector, flow cell、Digital to Analog Converter；

5、Pressure sensor (standard located behind the pump, in front of and behind the column);

6、Valve system (including bypass system valves, blowback system valves, and large volume collection valves);

7TheClearFirst-4500Type protein purification dedicated workstation；

Optional components:

1ThepHDetector (including flow cell, digital to analog converter);

2、Bubble detector (before system pump)/Before the sample pump/After loading the sample valve, the maximum4A);

3、Column selection valve;

4Chromatography freezer；

5Fractional collector.

ClearFirst -4500 Instrument Parameters:

1Solvent compatibility: commonly used aqueous buffer solutions for purifying biomolecules;

2System pump: dual pump with four inlet channels，High pressure constant flow pump，Flow rate range01200 mL/min；

3Pressure range: 1-600mL/minWhen,0.01-2MPa（20bar，145psi）；600mL/minWhen mentioned above,0-0.2MPa（2bar，30psi）；

4Sample pump: constant flow plunger pump, flow rate range0-600mL/min；

5Viscosity: up to5 cP；

6Wavelength Range:190-850 nm；

7Wavelength accuracy：±1 nm；

8Wavelength repeatability：±0.2 nm；

9、Optical path length：2 mm；

10Conductivity range:0-999.9 mS/cm；

11、7Channel large volume collection valve.

ClearFirst-4500 Instrument Purpose:

1、biomoleculeLike protein、nucleic acidWaiting for SeparationPurification (gel, ion exchange, hydrophobic interaction, affinity and other methods);

2Separation and purification of polymer impurities；

3Industrial grade production preparation.

This instrument is capable of performing various commonly used purification techniques，Such as affinity chromatography, ion exchange chromatographyHydrophobic chromatography, and gel filtrationWaitTherefore, this system can support various proteinsandPurification of nucleic acids, includingreorganizeProteins, antibodiesvaccinesOr natural proteinWait.

Due to continuous technological advancements, our company reserves the right to make design changes without prior notice. We apologize for any inconvenience caused.