Chromatographic Column Chemical CW553-C18-TH Rubia

MORHCHEM is a professional manufacturer of consumables related to chromatography columns. The company's headquarters is located in California, USA. After more than 30 years of continuous innovation and challenges, it has developed Caprisil，NexHgel，ItolSep，Guard column，Synersorb，Spherigel，HyperStar，Prep，CapriChiarl A series of HPLC chromatography columns.

MORHCHEM is committed to helping customers solve various challenges encountered in the field of analytical chemistry, from routine testing to complex research and development projects. Our clients include pharmaceutical and biotech companies, clinical diagnostic laboratories, universities, research institutes, and government agencies.

Chromatographic Column Chemical CW553-C18-TH Rubia

Caprisil C18-TH adopts an optimized bonding process to make the bonding phase more stable, less prone to detachment, with good pH stability (pH tolerance range of 1.5-10) and longer service life, making it very suitable for separating trace impurities.

Good peak shape, suitable for most compounds and changes in conditions;

Good pH stability;

Acid and alkali resistant, with a long service life;

Suitable for separating trace impurities.

Chromatographic Column Chemical CW553-C18-TH Rubia

Chromatography column: CW553-C18-TH

Flow rate: 1ml/min

Injection volume: 20ul

Mobile phase: methanol: acetonitrile: 0.2%H?PO?=25：47：28

Detector: UV@250nm

Sample spectrum:

Instructions for using reverse phase chromatography column

1、 Conventional flushing method under mobile phase conditions

1. Before use: If methanol water or acetonitrile water is used as the mobile phase, the sample can be directly equilibrated with the mobile phase for 40 minutes before baseline stabilization.

2. After use: Rinse with pure methanol or acetonitrile water (70:30) for more than 40 minutes and store.

2、 The mobile phase contains buffer salts

1. Transition before use: Transition with 90% water and organic phase for 40-60 minutes. Then, after 40 minutes of equilibration under mobile phase conditions, the sample is taken (if under gradient conditions, the initial gradient equilibrium is followed).

2. Rinse after use: Rinse with 90% water and organic phase for at least 60 minutes. Then rinse with pure methanol or acetonitrile water (70:30) for more than 40 minutes and store.

3、 The mobile phase contains ion pair reagents

1. Transition before use: Transition with 90% water and organic phase for 40-60 minutes. Then equilibrate the sample under mobile phase conditions for 120 minutes (if it is under gradient conditions, follow the initial gradient equilibrium).

2. Rinse after use: Rinse with 90% water and organic phase for at least 180 minutes. Then rinse with pure methanol or acetonitrile water (70:30) for more than 40 minutes and store.

Note:

1.Factory storage conditions:70：30=Acetonitrile: Water.

2.It is recommended to use a dedicated column for chromatography columns containing ion pair reagents.

3.It is recommended to store it in the factory solvent condition for long-term non use, and it is strictly prohibited to store it in a mobile phase containing salt.