Chemical analysis of rat nuclear magnetic resonance spectroscopy

The chemical analysis of rat nuclear magnetic resonance spectroscopy is a new technology with broad prospects in biomedical basic research and disease-related application research. Biomedical research based on animal models can avoid the risks brought by experiments on humans, overcome the disadvantages of long incubation period and disease progression of certain diseases, and strictly control animal experimental conditions to reduce the influence of individual differences.

　　Chemical analysis of rat nuclear magnetic resonance spectroscopyIt is a high-tech that can be widely applied in interdisciplinary fields such as materials science and biomedical basic research. It has broad prospects in both biomedical basic research and disease-related application research. Biomedical research based on animal models can avoid the risks of conducting experiments on humans, overcome the disadvantages of long incubation periods and disease courses of certain diseases, and strictly control animal experimental conditions to reduce the impact of individual differences. Imaging techniques, especially magnetic resonance imaging, are currently one of the most effective tools in animal model research. Currently, governments in Europe and America strongly support small animal magnetic resonance imaging research. The purchase of this system fully considers the needs of scientific research and practical applications, and can conduct cutting-edge research in morphology, spectroscopy, and functional imaging for small animals. It will further enhance the research level and status of scientific research institutions in this field. High field magnetic resonance animal imaging (Animal MRI) is a landmark analytical testing and research instrument used to measure the scientific research level and depth of research work in comprehensive hospitals. It has begun to develop in China and is becoming an analytical testing and research tool for teaching, scientific research, key disciplines, and key laboratory construction.

Our company has launched a 1.5T permanent magnet rat MRI system based on the current experimental requirements of domestic MRI equipment. The main technical parameters and experimental functions are as follows:

　　Chemical analysis of rat nuclear magnetic resonance spectroscopyMain technical parameters:

1. Magnetic field strength: 1.49T ± 0.02T

2. Resonance frequency: 59MHz ± 1MHz

3. Magnetic pole diameter: 300mm

*4. Effective sample diameter (probe coil) size: 50mm * 50mm * 80mm

*5. Experimental samples: rat whole space imaging experiment, contrast agent in vitro and in vivo experiment

*6. Magnetic field uniformity: less than 8ppm (50mm × 50mm × 80mm)

*7. Graphics resolution: Normal mode 128 × 128 × 128, maximum high-resolution 256 × 256 × 128

*8. Gradient magnetic field strength: 10gs/cm (1mT/cm or 100mT/m)

*9. Resolution: 0.08mm (based on a 0.05mm water model)

10. Image linearity: The X, Y, and Z directions are all better than 98% (50mm × 50mm × 80mm)

11. Maximum gradient magnetic field: 200mT/m in X, Y, Z directions

12. Temperature control stability: The temperature control accuracy of the chamber is ± 0.005 ℃; Display accuracy of 1m ℃

13. Magnetic field stability: The Larmor frequency drift per hour of magnetic field stability is less than 100Hz/h

14. Spatial resolution: Normal mode 0.15mm, High mode 0.08mm

Main experimental functions:

1. Research on T1/T2 magnetic resonance contrast agent relaxation measurement, in vitro and in vivo imaging of contrast agents in animals

2. In vivo magnetic resonance imaging of rats

3. Two dimensional spin echo T1 weighted graph, T2 weighted graph

4. Three dimensional gradient echo (3DGRE) imaging; 3D spin echo imaging experiment, 3D stereo imaging experiment

5. Two dimensional arbitrary angle multi-layer (MSE) imaging; Hard pulse CPMG pulse sequence measurement T2; Reverse Recovery (IR) pulse sequence measurement T1; Hard pulse measurement T2*

6. 3D imaging data acquisition and image inversion for 3D stereo reconstruction (pseudo color image reconstruction)

7. Can save experimental data in the standard file format for medical digital imaging and communication using DCOM

The main experimental content of the 1.5T MRI rat imaging research system (permanent magnet) is as follows:

1. It can conduct research on the principles of magnetic resonance imaging, imaging technology experiments, hardware structure experiments, and application expansion experiments.

2. Magnetic resonance imaging experiment, four-dimensional (molecular imaging) magnetic resonance spectroscopy imaging, three-dimensional spatial imaging.

3. Experimental study on the improvement and artifact research of magnetic resonance imaging, and the influence of various parameters of spin echo sequence on imaging performance.

4. Observation of research samples for magnetic resonance imaging (three-dimensional and two-dimensional imaging experiments of rats, small animals and plants), and research on molecular imaging of rats.

5. The measurement of relaxation time of experimental samples, multi angle observation and arbitrary angle storage of experimental sample images, magnetic susceptibility imaging and other related experiments, three-dimensional imaging data acquisition and image inversion, three-dimensional stereo reconstruction (pseudo color image reconstruction) can be widely applied in scientific research such as life sciences, medical imaging, biomedicine, and preclinical experiments of medicine.