Cell free system for cell-free protein synthesis equipment

Cell free system for cell-free protein synthesis equipment- eProtein Discovery

A rapid protein prototype system combining cell-free protein synthesis with digital microfluidics

In the fields of structural biology, drug development, and protein engineering, rapid, stable, and high-throughput expression of proteins has always been a core bottleneck. Traditional cell expression cycles based on prokaryotic or eukaryotic systems can last for weeks or even months, with cumbersome processes, poor reproducibility, and membrane protein aggregation and inactivation, seriously slowing down project progress.

Launched by the British company NucleraCell free system for cell-free protein synthesis equipment-EProtein Discovery is a cell-free system for in vitro transcription/translation, utilizing three core technologies: cell-free protein synthesis (CFPS), digital microfluidics, and fluorescent protein complementary detection. It creates an automated, high-throughput, microfluidic protein expression and purification screening system that can obtain high-purity active proteins from DNA within 48 hours, providing a revolutionary solution for drug target validation, AI protein design, and toxic protein/difficult to express protein/membrane protein (GPCR) research.

Two exclusive protein screening processes:

• Covered soluble protein categories: cytokines, transcription factors, kinases, antibodies and fragments (VHH/ScFv), RAS proteins, E3 ubiquitin ligases, regulatory proteins, and metabolism related proteins.

• Covered membrane protein categories: ion channels GPCR、 Transporters and membrane-bound enzymes, etc.

Soluble protein workflow:

Membrane protein workflow:

Multiple screening of different expression combinations:

It can systematically explore various expression conditions such as protein variants, soluble tags, redox conditions, molecular chaperones, metal ions, cofactors, and 3C protease in situ tag cleavage. Simultaneously providing optimized GPCR specific nanodisks to optimize the stability and expression levels of membrane proteins. We also support customer customized additive selection and provide guidance manuals.

The eProtein Discovery system has successfully expressed over 2000 proteins:

Including antibodies and antibody fragments, chaperone proteins, hydrolytic enzymes, ligases, oxidoreductases, signal proteins, structural proteins, and transferases, with a molecular weight range of 18kDA-300kDa.

Shanghai Manbo Biopharmaceutical Technology Co., Ltd. is a supplier for Nuclera in China, providing complete product sales, technical training, application support, and after-sales service.

Visit the Shanghai Manbo Biotechnology website to view and download relevant information on the Nuclea cell-free protein expression screening system.