Cell flow cytometry testing service

NegotiableUpdate on 05/06

Model

Nature of the Manufacturer: Producers

Product Category

Place of Origin

Online Consult

Overview

1. Immunophenotype analysis $r $n surface marker detection: recognizes cell surface proteins, such as CD molecules. Detection of intracellular markers: detecting cytokines, transcription factors, etc. $r $n2. Cell cycle analysis $r $nDNA content detection: Analyze each stage of the cell cycle by staining with propidium iodide (PI) or DAPI. Detection of proliferation markers such as Ki-67 and BrdU. $r $n3. Cell apoptosis detection $r $nAnnexin V/PI dual staining: distinguishing early and late apoptotic cells. $r $nCaspase activity detection: Evaluate apoptosis related enzyme activity. $r $n mitochondrial membrane potential detection

Product Details

1. Immunophenotype analysis

Surface marker detectionIdentify cell surface proteins, such as CD molecules.
Detection of intracellular markersDetect cytokines, transcription factors, etc.

2. Cell cycle analysis

DNA content detectionAnalyze each stage of the cell cycle by staining with propidium iodide (PI) or DAPI.
Proliferation marker detectionSuch as Ki-67, BrdU, etc.

3. Cell apoptosis detection

Annexin V/PI double stainingDistinguish between early and late apoptotic cells.
Caspase activity detectionEvaluate the activity of apoptosis related enzymes.
Mitochondrial membrane potential detectionUse dyes such as JC-1.

4. Cellular functional analysis

Calcium ion flow detectionUse dyes such as Fluo-3 and Fura-2.
Reactive oxygen species (ROS) detectionUse dyes such as DCFH-DA.
PH value detectionUse pH sensitive infectious agents.

5. Cell proliferation and differentiation

CFSE stainingTracking cell division.
Stem cell marker detectionSuch as OCT4, SOX2, etc.

6. Analysis of cellular signaling pathways

Phosphorylated protein detectionUse specific antibodies to detect signaling molecules.
Signal pathway activity detectionSuch as MAPK, PI3K/AKT, etc.