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Taizhou Sairui Cuike Biotechnology Co., Ltd
Celetrix upgraded cell electroporation analyzer
NegotiableUpdate on 05/06
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Overview
The features of the upgraded Celetrix cell electroporation analyzer are: simple interface, easy operation, and can be adapted to 20ul, 100ul, 200ul, 600ul, and 1ml electric shock tubes; $r $n2. Added cell line mode and PBMC mode for more convenient use; Retaining the traditional mode, users can set their own electroporation parameters based on experiments to achieve optimized transfection effects. The new mode optimizes the cell survival rate and transfection efficiency of large plasmid electroporation; $r $n3. Optional distribution integration mode.
Product Details
Celetrix adds new modes to simplify electrical conversion parameters
Celetrix upgraded cell electroporation analyzerDivide cell electroporation into two modes: cell line mode and PBMC mode.
The vast majority of cell lines, primary cells growing on the wall, as well as blood stem cells, stimulated T cells, B cells, NK cells, and other cells with larger diameters, have similar electrical conversion parameters, and can be converted by adjusting the voltage in cell line mode.
PBMC (peripheral blood mononuclear cells) as well as unstimulated T cells, B cells, and NK cells with smaller diameters require higher voltage. Voltage fine-tuning can be performed in PBMC mode. Electric to liquid conversion is universal in both modes, making it very convenient for users to use.
New pressurized electric shock tube
The electric shock tube is a brand new, efficient, and easy-to-use pressurized product;
Can withstand the pressure of gas generated by electric rotation, compress bubbles at the moment of electric rotation, and ensure the uniformity of the electric field;
Diversified volumes can meet different experimental needs, and simply replacing different adapters can adapt to different volumes of shock tubes.
Electric shock tube used in conjunction with electroporation solution, a reagent kit targeting all cells
Electric fusion hybridoma technology
Mouse hybridoma
Celetrix upgraded cell electroporation analyzerOptional cell fusion function, efficient and rapid,A mouse spleen can obtain a clone of 10E4
The cell electroporation instrument EX+and SLT can be optionally equipped with cell fusion function, which can efficiently fuse one mouse spleen cell at a time.
Electric conversion example
Stimulate the cultivation of T cell electroporation PB transposon
Observation under a 24-hour fluorescence microscope showed that the cells had significantly clustered for 5 days. Flow cytometry analysis showed a cell survival rate of 91.5% and an efficiency of 90.5%
The application of electroporation in the field of gene editing
Using Celetrix's electroporation technology to electroporate Cas9 protein and gRNA (+) or Cas9 protein in Human iPSCs, the gene editing efficiency was detected by T7E1 endonuclease,
In the APP, the editing efficiency of AAVS1, OCT4, and PDCD1 gene loci exceeds 50%, with AAVS1 gene locus editing efficiency reaching 70%
The application of electroporation in the field of antibodies
CHO cell electroporation efficiency of 6kb small plasmid>99% CHO cell 11kb large antibody mimic plasmid efficiency>80%
Transient expression of antibodies GFP and mCherry instead of HC and LC using ordinary plasmids
Efficient integration system for transposon expression of antibodiesContains GS screening markers for stable transformation of cell lines
Efficient transient transformation of small plasmids, rapid expression of proteins, high efficiency of electroporation of large plasmids, accelerating stable transformation progress
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