Bovine type I procollagen C-terminal propeptide (PICP) ELISA kit

Bovine type I procollagen C-terminal propeptide (PICP) ELISA kit

Price: 2600

Double antibody sandwich ELISA method

[Specification] 96T (detecting 84 samples)/48T (detecting 36 samples)

Humans, rats, mice, pigs, chickens, plants, rabbits, monkeys, etc

[ELISA Kit Product Usage]

For scientific research purposes only, to detect the activity or concentration of relevant indicators in serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other related samples.

Storage conditions and expiration date

1. Store at 2-8 ℃, do not freeze, expiration date is 6 months.

2. After opening and using, the package is wrapped in a microporous plate and placed in a self sealing bag with a desiccant. The self sealing bag is sealed and all reagents are placed back in the 2-8 ℃ refrigerator.

operating steps

1. Sample addition: Set up standard wells, blank wells (blank control wells do not add samples or enzyme-linked immunosorbent assay reagents, all other steps are the same), and test sample wells. Add 50 microliters to the standard well on the enzyme-linked immunosorbent assay (ELISA) coated plate, then add 40 μ l of sample diluent to the test sample well, followed by 10 μ l of the test sample (with a final dilution of 5 times). Add the sample to the bottom of the enzyme-linked immunosorbent assay plate well, avoiding touching the well wall as much as possible, and gently shake and mix well.

2. Enzyme addition: Add 50 μ l of enzyme labeled reagent to each well, except for blank wells.

3. Incubation: Cover the plate with a sealing film and incubate at 37 ℃ for 60 minutes.

4. Solution preparation: Dilute 30 times the concentrated washing solution with distilled water and set aside for later use

5. Washing: Carefully remove the sealing film, discard the liquid, shake dry, fill each hole with washing solution, let it stand for 30 seconds, then discard. Repeat this process 5 times and pat dry.

6. Color development: Add 50 μ l of color developing agent A50 to each well, then add 50 μ l of color developing agent B50, gently shake and mix well, and develop color at 37 ℃ in the dark for 15 minutes

7. Termination: Add 50 μ l of termination solution to each well to terminate the reaction (at this point, blue will turn yellow).

8. Measurement: Zero the blank well and measure the absorbance (OD value) of each well in sequence at a wavelength of 450nm. The measurement should be conducted within 15 minutes after adding the termination solution.