Bipu Activated Sludge Breather

Application of Microbial Breath Measurement Method in Monitoring Water Pollutants

Urban sewage treatment plants mainly use activated sludge technology to treat pollutants in wastewater and combine it with anaerobic digestion technology to achieve the reduction of primary and residual sludge. That is, the physiological metabolic activities of microorganisms in activated sludge are used to remove organic pollutants in wastewater, while anaerobic microorganisms are used to decompose the organic matter in primary and residual sludge into methane and carbon dioxide, thereby achieving the overall goal of removing carbon containing organic matter and ammonia nitrogen in wastewater, reducing sludge, and resource utilization. The removal of carbon containing organic matter and ammonia nitrogen is a microbial metabolic process that requires oxygen consumption, while the digestion of sludge is a microbial anaerobic metabolic process. The biochemical oxygen demand (BOD) can be calculated by measuring the oxygen consumption required by aerobic bacteria for degrading organic matter in wastewater; By detecting the respiration rate of aerobic microorganisms in activated sludge, the oxygen utilization efficiency (OUR) of activated sludge can be analyzed; Determine the methane potential (BMP) of initial sedimentation and residual sludge by measuring the amount of methane generated; Assessing residual greenhouse gas potential (RGP) by detecting the amount of residual gas generated from anaerobic digestion of biogas slurry and sludge; The activity of anaerobic ammonia oxidizing bacteria (SAA) under specific conditions can be evaluated by measuring the release of nitrogen gas; The toxicity of relevant substances can also be evaluated by analyzing the inhibitory effect of potential toxic and harmful substances on microbial respiration rate.

Application field

Activated sludge activity analysis

Microbial respiration meter Gas Endeavour®III can continuously and online monitor the released gas based on the principle of gas volume measurement. This instrument is an ideal analytical tool for conducting the following batches of activity tests:

1) Batch Denitrification Activity Analysis - This test aims to evaluate the maximum denitrification rate and anaerobic microbial growth benefits of sludge samples.

2) Batch anaerobic ammonia oxidation activity analysis - This test is used to evaluate the activity of anaerobic ammonia oxidizing bacteria (SAA) under specific conditions, or to study the toxic effects of different compounds on SAA by recording the nitrogen released by anaerobic ammonia oxidizing biomass.

Microbial respiration assay method

Gas Endeavour®III is a fully automatic breathing apparatus that uses the volumetric method for continuous gas volume measurement. It can be used in conjunction with our product's in-situ carbon dioxide adsorption device as a closed respiration meter for microbial respiration rate analysis. A complete instrument configuration can provide practical solutions for anaerobic and aerobic respiration measurements using biomass and wastewater samples.

1) Anaerobic microbial respiration assay - continuously monitoring methane production rate and reflecting processes with limited gas production rate (usually hydrolysis in complex substrates).

2) Aerobic microbial respiration assay - can continuously monitor oxygen consumption without limiting oxygen supply by combining Gas Endeavour and in-situ carbon dioxide adsorption device.

3) Batch testing for wastewater characteristics - including biochemical methane potential (BMP) determination for anaerobic wastewater treatment and biochemical oxygen demand (BOD) determination for evaluating organic matter removal and receiving wastewater concentration in wastewater treatment plants.

4) Batch testing for microbial characteristics - including determination of specific methane production rate (SMA) to evaluate the biological metabolic activity of activated sludge in terms of oxygen utilization efficiency (OUR) and specific oxygen consumption rate (SOUR).

Technical Specifications

Training Unit - Standard Version

• The maximum number of reactors in the system: 18 or 9

• Reactor material: Glass

• Standard reactor capacity: 500 ml or 1000 ml

• Heating typeConstant temperature water bath

• size68 x 56 x 33 cm (constant temperature water bath)

• temperature controlUp to 60 ° C (140 ° F) with an accuracy of 0.2 ° C

• Reactor stirringMulti functional mechanical mixer, using brushless DC motor (adjustable interval, speed, and rotation direction), with a maximum speed of 220 revolutions per minute

Cultivation Unit - Animal Nutrition Edition

•Maximum number of reactors per system: 18 or 9

•Reactor material: Glass

•Standard reactor capacity：250 ml

•Heating typeConstant temperature shaker water bath

•size53 x 33 x 28 cm (constant temperature water bath)

•temperature controlUp to 99 ° C (210 ° F)

Carbon dioxide adsorption unit

•CO2 adsorption bottle18 and 9

•Capacity of carbon dioxide adsorption bottle： 250 ml

•Bottle holder size: 55 x 28 x 17 (two groups and one group)

•Recommended adsorbent solution3 M NaOH (including pH indicator *), 200ml per bottle (not included)

•Absorption efficiency：>98%

*The standard configuration of the instrument does not include recommended adsorption solution

BPC Core Core Unit

•working principleGas liquid displacement and buoyancy

•Number of gas measurement chamber units: 18 or 9

•unit size: 55 x 19 x 17 cm

•Built in sensorsTemperature, pressure, magnetic force, level gauge

•interfaceEthernet, power supply USB B、 motor control

•display screenOLED screen 2.8-inch 256 x 64

•shellAluminum alloy and plastic

•power supply: 12V DC/1.0A, 100-240V AC

•Applicable Environment: Indoor

•measuring mediumDeionized water and distilled water

•Operating temperature: 0 – 50℃

•Operating pressure: -50 – 50 mbar

•Diameter of inlet and outlet: ID: 2.4 – 2.6 mm; OD: 4.2 – 4.7 mm; Recommended tracheal diameter: Inner diameter ID: 4 mm; Outer diameter OD: 6 mm

•Measurement resolution:2ml or 9ml

•Testing capacityEach channel of the 9ml flow chamber unit can cumulatively measure 7200L of gas; Each channel of the 2ml flow chamber unit can cumulatively measure 1600L of gas

•measurement range: 1-6000 ml/h (9ml flow chamber unit); 0.2-1500 ml/h (2ml flow chamber unit)

•repetitivenessCV ≤ 1% (9ml flow chamber unit); CV ≤ 3% （2mlFlow chamber unit

•Applicable gasesNon corrosive gases (such as CH4, CO2, H2, N2, etc.)

Bipu Instrument (Zhejiang) Co., Ltd. provides you with information on the parameters, price, model, principle, etc. of Gas Endeavour III activated sludge analyzer. The price is negotiable, and you can contact us for more related informationconsulting service