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Shanghai Aiyan Biotechnology Co., Ltd
Bio rad dual 48 well gradient PCR instrument C1000
NegotiableUpdate on 05/06
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Overview
The bio rad Bole dual 48 well gradient PCR instrument C1000 has replaceable reaction modules, including 96x0.2ml gradient single slot module, 2x48x0.2ml dual slot gradient module, 384 well single slot gradient module, quantitative PCR gradient module, etc.
Product Details
Performance description of bio rad dual 48 well gradient PCR instrument C1000
Equipped with replaceable reaction modules, including 96x0.2ml gradient single slot module, 2x48x0.2ml dual slot gradient module, 384 well single slot gradient module, quantitative PCR gradient module, etc
8.5 High resolution ultra large color LCD display screen, with options for graphic programming, text programming, or automatic programming
Dual slot temperature gradient module, capable of independently running two different programs
Equipped with dynamic temperature gradient function, it can operate at 8 different temperatures simultaneously, with the same incubation time for each temperature
Semiconductor heating and cooling method
Bio rad dual 48 well gradient PCR instrument C1000 is mainly used for in vitro nucleic acid fragment amplification with dynamic temperature gradient function
Technical parameters of Bole C1000PCR instrument:
96 blue-green LED sequentially excited samples greatly reduced fluorescence crossover
Suitable for 96 × 0.2ml reaction tubes or 12 0.2ml 8-linked reaction tubes.
It can support multiplex PCR, which not only saves reagents and time, but also makes it possible to use internal controls, thereby further controlling the efficiency of sample PCR.
perfectTemperature control performance and gradient PCR function.
OpticonMonitor analysis software can easily set up reactions such as RT-PCR and multiplex PCR, as well as perform functional analyses such as DNA quantification, gene expression, and allele analysis.
Accurate and reliable PCR instrument:
Opticon's C100PCR instrument is based on the mature Bio Rad product PTC-200, which features semiconductor refrigeration technology;
Three temperature sensors, dual zone temperature control;
Bole C1000PCR instrument
Accurate temperature control: The accuracy is ± 0.3 ℃ within 30 seconds at 90 ℃, even considering the influence of PCR reaction solution volume on temperature changes;
Good uniformity: Within 30 seconds at 90 ℃, the uniformity is ± 0.4 ℃;
96 reaction sample sizes;
Fast temperature rise and fall speed: up to 3.0 degrees per second;
There is a calculated temperature control mode: during the temperature rise and fall process, it does not exceed the head, thus ensuring the repeatability of the experiment;
Temperature gradient performance: The sample module can have 12 different temperatures simultaneously from left to right, forming a temperature gradient. The gradient temperature difference is set to a minimum of 1 ℃ and a maximum of 24 ℃. Through one experiment, the annealing temperature or denaturation temperature can be determined, which not only saves time but also reduces costs.
Fluorescence detection system combining dynamic and static detection:
Four independent excitation channels are excited using light sources of different wavelengths (light-emitting diodes, LEDs) to achieve the highest excitation efficiency for each channel;
Four independent detection channels use detectors with different photosensitive ranges (photodiodes, PDTs) for detection, achieving the highest emission detection efficiency for each channel;
The light source does not generate heat, has a long service life, and does not need to be replaced for life;
The position of the light source and detector is relatively fixed, ensuring that the optical path * is consistent and * between the holes
Interference issues;
The quantitative PCR instrument with the shortest optical path, with a total optical path of less than 8cm and the least light propagation loss, detects over 10 fluorescent dyes including FAM, SYBRGreenI, VIC, HEX, Joe, TAMRA, Cy3, Cy5, TexasRed, Rox, etc.
Wavelength range: 450-730nm;
Wide linear range: up to 10 orders of magnitude, capable of detecting single copy templates;
The resolution of Jijia can distinguish between 32, 64, 128, 256, and 1024 copies;
technical parameters
Note: Only 96 hole and dual 48 hole heating modules have temperature gradient function.
Technical parameters of the adapted heating module
Equipped with replaceable reaction modules, including 96x0.2ml gradient single slot module, 2x48x0.2ml dual slot gradient module, 384 well single slot gradient module, quantitative PCR gradient module, etc
8.5 High resolution ultra large color LCD display screen, with options for graphic programming, text programming, or automatic programming
Dual slot temperature gradient module, capable of independently running two different programs
Equipped with dynamic temperature gradient function, it can operate at 8 different temperatures simultaneously, with the same incubation time for each temperature
Semiconductor heating and cooling method
Bio rad dual 48 well gradient PCR instrument C1000 is mainly used for in vitro nucleic acid fragment amplification with dynamic temperature gradient function
Technical parameters of Bole C1000PCR instrument:
96 blue-green LED sequentially excited samples greatly reduced fluorescence crossover
Suitable for 96 × 0.2ml reaction tubes or 12 0.2ml 8-linked reaction tubes.
It can support multiplex PCR, which not only saves reagents and time, but also makes it possible to use internal controls, thereby further controlling the efficiency of sample PCR.
perfectTemperature control performance and gradient PCR function.
OpticonMonitor analysis software can easily set up reactions such as RT-PCR and multiplex PCR, as well as perform functional analyses such as DNA quantification, gene expression, and allele analysis.
Accurate and reliable PCR instrument:
Opticon's C100PCR instrument is based on the mature Bio Rad product PTC-200, which features semiconductor refrigeration technology;
Three temperature sensors, dual zone temperature control;
Bole C1000PCR instrument
Accurate temperature control: The accuracy is ± 0.3 ℃ within 30 seconds at 90 ℃, even considering the influence of PCR reaction solution volume on temperature changes;
Good uniformity: Within 30 seconds at 90 ℃, the uniformity is ± 0.4 ℃;
96 reaction sample sizes;
Fast temperature rise and fall speed: up to 3.0 degrees per second;
There is a calculated temperature control mode: during the temperature rise and fall process, it does not exceed the head, thus ensuring the repeatability of the experiment;
Temperature gradient performance: The sample module can have 12 different temperatures simultaneously from left to right, forming a temperature gradient. The gradient temperature difference is set to a minimum of 1 ℃ and a maximum of 24 ℃. Through one experiment, the annealing temperature or denaturation temperature can be determined, which not only saves time but also reduces costs.
Fluorescence detection system combining dynamic and static detection:
Four independent excitation channels are excited using light sources of different wavelengths (light-emitting diodes, LEDs) to achieve the highest excitation efficiency for each channel;
Four independent detection channels use detectors with different photosensitive ranges (photodiodes, PDTs) for detection, achieving the highest emission detection efficiency for each channel;
The light source does not generate heat, has a long service life, and does not need to be replaced for life;
The position of the light source and detector is relatively fixed, ensuring that the optical path * is consistent and * between the holes
Interference issues;
The quantitative PCR instrument with the shortest optical path, with a total optical path of less than 8cm and the least light propagation loss, detects over 10 fluorescent dyes including FAM, SYBRGreenI, VIC, HEX, Joe, TAMRA, Cy3, Cy5, TexasRed, Rox, etc.
Wavelength range: 450-730nm;
Wide linear range: up to 10 orders of magnitude, capable of detecting single copy templates;
The resolution of Jijia can distinguish between 32, 64, 128, 256, and 1024 copies;
technical parameters
| C1000 | |
| display screen | 12 x 9 cm, High resolution, full-color |
| interface | 5 USB-A |
| 1 USB-B | |
| Compatible with USB flash memory | is |
| Compatible with USB mouse | is |
| Programming Options | Graphics, Text, Automatic Programmer |
| Automatic programmer | is |
| report | Can output running logs and system error logs |
| safety performance | Protection mode, login options, folder protection |
| Temperature control mode | Logic algorithm and module temperature control |
| Upgrade to a quantitative PCR instrument | is |
| PCR certificate | have |
| Online software | Windows CE 6.0 |
| Adapt to workstations | is |
| store | More than 1000 programs, USB expansion |
| heat preservation function | have |
| Size (W X D X H) | 13”x18”x8” |
| weight | 23 lbs |
| Temperature gradient accuracy* | ±0.2°C |
| Uniformity of temperature gradient rows* | ±0.4°C? |
| Temperature gradient range* | 30-100 ° C, not lower than room temperature of 30 ° C |
| Temperature gradient temperature difference range | 1-24°C |
| voltage range | 100-240VAC (voltage does not need to be adjusted within this range) |
| frequency | 50–60 Hz |
| output power | zui大 400 W |
| fuse | 2, 6.3 A, 250 V, 5 x 20 mm |
Note: Only 96 hole and dual 48 hole heating modules have temperature gradient function.
Technical parameters of the adapted heating module
| 96 hole quick module | 48/48 hole quick module | 384 holes | |
| Sample capacity | 96x 0.2mL | 2x48x 0.2mL | 1 384 well reaction plate |
| Temperature gradient function | have | have | nothing |
| Temperature gradient range | 30-100°C | X | |
| Temperature gradient temperature difference range | 1-24°C | X | |
| Temperature gradient accuracy | ±0.2°C | X | |
| Uniformity of temperature gradient | ± 0.4 ° C (maintained for 10 seconds at 90 ° C) | X | |
| Hot cover temperature range | 0-110°C | ||
| The maximum rate of temperature increase and decrease | 5 ° C/second | 4 ° C/second | 2.5 ° C/second |
| temperature range | 0-100°C | ||
| Temperature accuracy | ±0.2°C | ||
| Temperature uniformity | ± 0.4 ° C (reaching 90 ° C in 10 seconds) | ||
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