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No. 29 Qingxiang South Road, Daxing District, Beijing
Beijing Punard Technology Co., Ltd
No. 29 Qingxiang South Road, Daxing District, Beijing
United StatesBD 210950Sabouraud glucose agar(SDA)
Sabouraud glucose agar(SDACulture medium
Sabouraud Dextrose Agar
210950
:500G, each47.5Prepare 1L of culture medium with g
Used for the isolation and cultivation of fungi (yeast, mold), especially suitable for the detection of fungi in clinical, environmental, and food samples.
Cultivation medium formula:
glucose:40.0 g/L (providing carbon source)
Protein peptone:10.0 g/L (providing nitrogen source)
Agar:15.0 g/L (coagulant)
PH: 5.6 ± 0.2 (acidic environment inhibits bacterial growth)
Preparation method:
Dissolve 47.5g of dry powder in 1L of pure water.
2. Heat and boil until dissolved.
3. Sterilize under high pressure at 121 ° C for 15 minutes.
4. After cooling to 45-50 ° C, invert onto a flat or sloping surface.
culture conditions:
temperature20-25 ° C (normal temperature fungi) or 30-37 ° C (some pathogenic fungi).
Time: Usually48 hours to 7 days (mold may take longer).
quality control:
Candida albicans(Candida albicans ATCC 10231: Good growth.
Escherichia coli(E. Coli ATCC 25922): inhibited.