B1 Aspergillus flavus colloidal gold detection card

The B1 Aspergillus flavus colloidal gold detection card is a POCT (point of care) product in the field of food safety, constructed using colloidal gold immunochromatography. It is used for on-site detection (or rapid laboratory detection) of Aspergillus flavus B1 in grains, cereals, and feed. The Aspergillus flavus B1 detection card product has high sensitivity, good specificity, and easy operation, and does not require any instruments or equipment. The results can be directly read by the naked eye.

Aspergillus flavus B1 detection card (50 pieces/box)

Disposable plastic dropper

desiccant

1 bottle of sample diluent

Instruction manual (1 copy/box)

Sample Processing

(a) Simple Sample Direct Extraction Method

Grind feed, grains (rice, wheat, corn, peanuts) and other samples, weigh 3.0 g of the crushed sample, add 7 mL of sample extraction solution (extraction solution preparation: 50 mL methanol+50 mL water, add 4 g sodium chloride and mix well to obtain the sample extraction solution, which can be prepared as needed), stir and mix thoroughly for 2 minutes, let it stand for 20 minutes or centrifuge at 4000 rpm for 5 minutes, take the supernatant, dilute according to the table below to achieve the desired detection range:

(b) Complex sample drying/blow drying method (detection limit: 20 ppb)

Grind feed, grains (rice, wheat, corn, peanuts) and other samples, weigh 2.0 g of the crushed sample, add 16 mL of sample extraction solution (extraction solution preparation: first dissolve 4 g of sodium chloride in 20 mL of water, then add 80 mL of methanol to obtain the sample extraction solution, which can be prepared as needed), stir and mix thoroughly for 2 minutes, let it stand for 20 minutes or centrifuge at 4000 rpm for 5 minutes, gently take 1 mL of the supernatant, dry it in a 100 ℃ oven or blow dry it with 50-60 ℃ nitrogen gas, dissolve it fully in 0.5 mL of 10% methanol, and then proceed with sample testing.

【 Instructions 】

1. Carefully read the user manual and preheat the test board, processed samples, and standards to room temperature (20-30 ℃) before testing. Do not open the aluminum foil bag before preparation, and use it within one hour after opening.

2. Take out the detection board and disposable dropper from the packaging bag and place them on a horizontal tabletop. Mark the sample (or standard) number on the detection board.

3. Slowly and vertically drip 2 drops of sample (approximately 50 μ L) into the sample well using a disposable pipette, and start timing after adding the sample. After adding the sample, do not move the detection plate and read the results for 8-10 minutes.

【 Result judgment 】

Negative (-): The T-line (detection line, near the end of the sample well) shows color, indicating that the concentration of Aspergillus flavus B1 in the sample is below the detection limit or does not contain Aspergillus flavus B1.

Positive (+): If there is no color development on the T-line, it indicates that the concentration of Aspergillus flavus B1 in the sample is higher than the detection limit.

Invalid result: No C-line (control line) appeared, which may be due to improper operation or detection board failure. The instruction manual should be read again and retested with a new testing board.

【 Precautions 】

Do not touch the white film surface in the center of the detection board;

Do not use expired detection boards;

Do not reuse the equipped dropper to avoid cross contamination;

Do not consume the provided reagents.