Ausbian premium fetal bovine serum (South American blood source)

AUSB PremiumFetal Bovine Serum（南美血源）

Ausbian Fetal Bovine Serum，Sterile

Batch number: see bottle body

Validity period: 5 years

Overview of bovine serum products

Bovine serum is an important natural additive in cell culture experiments, containing abundant nutrients necessary for cell growth, and is commonly used for in vitro culture of animal cells. According to different blood collection times, bovine serum can be divided into fetal bovine serum, newborn bovine serum, calf serum, and adult bovine serum.

Foetal Bovine Serum (FBS): It is fetal blood collected from 5-8 month old bovine embryos. At this time, the various organs of the fetal cow are in the stage of growth and differentiation, and the blood contains abundant growth and development factors, which are very suitable for the cultivation of various cells. Once the embryo develops, these factors automatically disappear. Therefore, bovine embryos over 8 months of gestational age, which are close to full-term, cannot be used as raw materials for fetal bovine serum.

Newborn Calf Serum (NBCS): within 07 days (some say within 030 days);

Calf Serum: collected from calves born 30 days and 1 year ago.

Adult Bovine Serum (ABS): Serum isolated from whole blood collected from adult cattle.

The content of endotoxins affects the quality of cell culture

All serum should undergo strict quality control before leaving the factory, and some physiological and biochemical indicators should be tested. Among them, it is necessary to focus on the indicator of endotoxins. Due toEndotoxins directly participate in cell apoptosisIt has a significant impact on the results of cell culture, and the differences in endotoxins between different batches of serum are difficult to control. Therefore, when selecting serum, attention should be paid to the endotoxin content, especially for those who have the following conditions, and special attention should be paid to screening for serum endotoxins:

When cultivating stem cells, they are highly sensitive to endotoxins. If the endotoxin level in the serum is ≥ 3EU/ml, stem cells are prone to death. Many users review the batch of 'test reports' in advance before testing the serum to determine whether it is eligible for trial.

When nourishing immune cells, excessive endotoxins can induce immune cells to release inflammatory factors, inhibit the formation of mouse red blood cell colonies, and so on.

3. In cell experiments related to gene knockout, cultured cells should be kept in their original state as much as possible. Any reagents that guide cell aging or apoptosis will cause subsequent experimental results to be "lost even by a small margin". When preparing serum and other reagents, it is crucial to choose extremely low endotoxins (≤ 1EU/ml) for the experiment;

4. Primary culture, hybridoma fusion, cell transfection, or expansion of difficult to maintain cells such as liver cells, nerve cells, and endothelial cells all require selected serum to provide strong support to the cells. Therefore, selecting lower endotoxins is the * * step to ensure a smooth experiment.

5. Some cells in the laboratory require long-term cultivation, long-term freezing, or frequent resuscitation and cultivation, and serum will often or for a long time act on the cells. To ensure the health of cells, serum with lower endotoxins should be used to avoid the long-term toxic effects of endotoxins on cells.

In short, excessive levels of endotoxins in serum can easily lead to cellular "sub-health", resulting in inaccurate data, poor cell status, aging, and even death, leading to experimental interruption and failure. The lower the endotoxin in the serum, the better. The Global Society for Cell Culture classifies and names serum quality based on endotoxins.

The endotoxin content is at the serum level

The lower the endotoxin content in the serum, the higher the level. Taking fetal bovine serum as an example:

When the endotoxin content in the serum is ≤ 10EU/ml, this serum is classified as special grade fetal bovine serum;

When the endotoxin content in the serum is greater than 10EU/ml and less than or equal to 25EU/ml, this serum is considered high-grade fetal bovine serum;

When the endotoxin content in the serum is ≥ 25EU/ml, this serum is standard grade fetal bovine serum;

At present, due to significant differences in endotoxin values between different batches of serum from the same manufacturer, many serum manufacturers no longer indicate the level in their names. But users can still identify and select by themselves through the testing report.

Scope of application:

Ausbian premium fetal bovine serum (South American blood source)Selected batches of fetal bovine serum with lower endotoxin levels from South American blood sources, with endotoxin content ≤ 3EU/ml, can be used for the cultivation of conventional cells, such as passaged cell lines, tumor cell lines, and some primary cell lines, providing nutritional support for in vitro cell culture experiments.

Ausbian premium fetal bovine serum (South American blood source)Advantage:

1. Select lower batches of endotoxins (≤ 3EU/ml) imported from South American blood sources

2. Provide pre trial services for more peace of mind in cell cultivation.

3. Provide free proxy storage services for Ausbian imported serum, provide reliable serum storage protection for laboratories with insufficient storage space, and ship as required at any time.

4. Ausbian serum is protected by low-temperature cold chain from the factory to the laboratory, ensuring that the serum does not undergo repeated freezing and thawing.

5. Free genuine product guarantee, Viansaga sticker added to each serum bottle body ® Easy to break anti-counterfeiting labels, Ausbian brand genuine product protection, and strict investigation for any counterfeit products, providing after-sales support for the laboratory.

6. Free professional assistance, gathering nearly 20 years of experience in cell culture from nearly a thousand serum users, providing problem analysis and solution references for various cell cultures.