Arabidopsis cultivation room

name;Arabidopsis cultivation room

Capacity: 1000L

Dimensions: 1250 × 650 × 1980

Studio size: 1200 × 600 × 1370

Temperature control range and accuracy: 0-50 ℃± 0.1

Temperature fluctuation: ± 0.5 ℃

Temperature uniformity: ± 1 ℃

Illuminance: 0-20000LX (Arabidopsis plant growth lamp)

Humidity control range: 50-95% RH

Humidity fluctuation: ± 5% RH

Heating power: 800W

Compressor power: 350W

Compression maneuver delay protection time: about 3 minutes

Refrigerant: R12 or R134a (fluorine free)

Working environment: Temperature range of 0-40 ℃, no corrosive gases

Power supply: 220 ± 22V, 50 ± 0.5HZ
Arabidopsis cultivation roomArabidopsis thaliana is a winter annual plant with a relatively short life cycle, and it is a popular model organism in the fields of plant biology and genetics. Achieve a high level of flexibility, precision, and versatility.

Growth characteristics of Arabidopsis thaliana

The effect of light on Arabidopsis growth involves two aspects: light intensity and photoperiod. In terms of light intensity, the optimal light intensity in the growth chamber is generally 120-150 μ mol/(㎡· s) (8000-12000LX), which can be achieved by using fluorescent lamps combined with incandescent light. In summer greenhouses, 60% shade helps with light intensity control and temperature regulation. High light intensity or direct sunlight exposure can be tolerated by older plants, while young plants avoid strong light.

Arabidopsis flowering accelerates under continuous light and long days, while flowering is inhibited or delayed under short days, indicating that Arabidopsis flowering requires a long photoperiod of at least 12 hours of light. In winter, the greenhouse can supplement morning and evening light to meet the needs of the light cycle. Generally, a 16 hour light period and an 8-hour dark period are recommended. Continuous light can promote reproductive cycles, slightly advance flowering, but reduce leaf number and seed generation, while shorter days are beneficial for nutritional growth.

temperature

The temperature of the greenhouse and growth chamber should be controlled, with a suitable growth temperature of around 25 ℃, and slightly lower temperatures are also allowed. When there is sufficient water supply, plants can even grow up to 34 ℃, but fertilization will be reduced. Older plants can tolerate high temperatures, but maintaining 25 ℃ is beneficial for the entire growth cycle. When seeds are formed, the temperature in the growth chamber should be set at 25 ℃, while the temperature in the greenhouse should be set at 23 ℃. The night temperature should be 2-4 ℃ lower than the day temperature. [3]

For many late flowering Arabidopsis ecotypes, the seedling stage needs to be treated at around 4 ℃ for a period of time (such as several weeks) to complete vernalization and promote flowering under long days. For commonly used Arabidopsis ecological types Landsberger ecta and Columbia, flowering can be achieved without vernalization treatment. It must be noted that the low-temperature vernalization treatment here is different from the low-temperature treatment used to break dormancy during sowing, which is also known as stratification treatment.

水分

In the first few weeks after seed germination, the ideal water supply comes from capillary water seepage from bottom to top, and only timely irrigation when the soil is dry. Excessive water supply can cause the growth of algae and fungi on the soil surface. Drought must be avoided before the first two true leaves of Arabidopsis begin to extend. After the true leaves grow, the frequency of watering can be reduced accordingly, such as once or twice a week. During the filling stage of the long horned fruit, water supply must be ensured to facilitate seed formation. Watering should be done after about 90% of the plug or pot has dried. Not only does soil water supply affect the growth and development of Arabidopsis, but humidity also affects water supply. Although an increase in humidity (such as 50% to 60%) can greatly reduce the effects of soil surface drought and the dehydration hazards of sprouting seedlings, generally speaking, Arabidopsis plants, including seedlings, can tolerate low humidity. Plants in the rosette stage can grow under different humidity levels. When the pods enter the mature stage, lower humidity (such as<50%) is advantageous.