Anti OV Ab ELISA kit in stock

Anti OV Ab ELISA kit in stock

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ELISAThe basic principle of the method is:①Make antigens orantibodyBind to the surface of a solid carrier and maintain its immune activity.②Linking an antigen or antibody to an enzyme to form an enzyme-linked antigen or antibody that retains both its immune activity and enzyme activity. When measuring, take the tested specimen(Determine the antibodies or antigens present in it)Reacts with the antigen or antibody on the surface of the solid-phase carrier in different steps with the enzyme-linked antigen or antibody. Separate the antigen antibody complex formed on the solid-phase carrier from other substances by washing,The amount of enzyme bound to the solid-phase carrier is proportional to the amount of the test substance in the specimen. After adding the substrate to the enzyme reaction, the substrate is catalyzed by the enzyme to become a colored product, and the amount of the product is directly related to the amount of the analyte in the specimen. Therefore, qualitative or quantitative analysis can be conducted based on the intensity of the color reaction. Due to the high catalytic frequency of enzymes, the reaction effect can be greatly amplified, thereby achieving high sensitivity in the measurement method. Therefore,ELISADetection is a process of localization, qualitative and quantitative analysis(Sensitivity can reach per milliliterng~pglevel)Comprehensive technology. The commonly used enzyme is horseradish peroxidase(HRP)And alkaline phosphatase(AKP)

ELISASample preparation and requirements：

1. Serum: Natural coagulation of blood at room temperature10-20Minutes, centrifuge20About minutes（2000-3000turn/Divided). Carefully collect the supernatant. If precipitation occurs during storage, centrifuge again.

2. Plasma: should be selected according to the requirements of the specimenEDTAOr sodium citrate as an anticoagulant, mixed10-20After minutes, centrifuge20About minutes（2000-3000turn/Divided). Carefully collect the supernatant. If sediment forms during storage, it should be centrifuged again.

3. Urine: Collect with sterile tube, centrifuge20About minutes（2000-3000turn/Divided). Carefully collect the supernatant. If sediment forms during storage, centrifuge again. Refer to the implementation for pleural effusion, ascites, and cerebrospinal fluid.

4. Cell culture supernatant: When detecting secreted components, collect them using sterile tubes. centrifugation20About minutes（2000-3000turn/Divided). Carefully collect the supernatant. When detecting the components inside cells, usePBS（PH7.2-7.4）Dilute the cell suspension until the cell concentration reaches100ten thousand/mlabout. By repeatedly freezing and thawing, cells are destroyed and intracellular components are released. centrifugation20About minutes（2000-3000turn/Divided). Carefully collect the supernatant. If precipitation forms during storage, it should be centrifuged again.

5. Tissue specimen: After cutting the specimen, weigh it. Add a certain amountPBS，PH7.4Quickly freeze and store in liquid nitrogen for future use. The specimen remains intact even after melting2-8The temperature is ℃. Add a certain amountPBS（PH7.4）Use manual or homogenizer to homogenize the specimen thoroughly. centrifugation20About minutes（2000-3000turn/Divided). Carefully collect the supernatant. After packaging, one portion is to be tested, and the rest is to be frozen for future use.

6. Extract the specimen as soon as possible after collection, according to relevant literature, and conduct experiments as soon as possible after extraction. If the experiment cannot be conducted immediately, the specimen can be placed in-20Store at ℃, but avoid repeated freeze-thaw cycles.

7. Cannot detect containingThe sample of NaN3, due toNaN3Inhibition of horseradish peroxidase（HRP）Activity.

Anti OV Ab ELISA kit in stock

Precautions for operation:

● Reagents should be stored according to the label instructions and returned to room temperature before use. The diluted standard should be discarded and cannot be stored.

● The Flat noodles not used in the experiment shall be immediately put back into the packaging bag and sealed for storage to avoid deterioration.

● Other unused reagents should be packaged or covered. Do not mix reagents of different batches. Use before shelf life.

● Use disposable suction tips to avoid cross contamination and extract termination solution and substrateATheBWhen using liquid, avoid using a sampler with metal parts.

● Use clean plastic containers to prepare detergent. Mix all components and samples in the reagent kit thoroughly before use.

● substrateAIt should evaporate and avoid opening the lid for a long time. substrateBSensitive to light, avoid prolonged exposure to light. Avoid contact with hands, toxic. After the experiment is completed, it should be read immediatelyODValue.

● The order of adding reagents should be*To ensure that the incubation time for all reaction plate pores is the same.

● Perform incubation operations according to the time, amount, and sequence indicated in the instructions.

Disclaimers

1. The reagent kit is for research use only and should not be used for clinical trials orpersonPhysical experiments, otherwise all consequences arising shall be borne by the experimenter, and our company shall not be responsible.

2. Strictly follow the instructions for operation. If the experimenter violates the instructions, the consequences shall be borne by the experimenter.

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