Anaerobic microaerophilic cultivation

Product Introduction:

DW-100A-K series anaerobic microaerophilic cultivationThe instrument is widely used for the cultivation of anaerobic bacteria (such as Clostridium perfringens, Lactobacillus, Bifidobacterium) and microaerophilic bacteria (such as Campylobacter jejuni) required for food safety standard testing; Specific oxygen concentrations (1% -15% optional) and CO2 concentrations (5% -15% optional) can also be selected according to the needs of scientific research experiments to study the optimal cultivation conditions for anaerobic bacteria. It can be widely used in various inspection places such as disease control, market supervision and inspection institutions, scientific research laboratories, food enterprises, etc.

Application principle:

The system adopts the "Flushing&Gassing" gas displacement method, which currently generates anaerobic/microaerophilic conditions, to create a culture gas environment. The system is equipped with a precision oil-free vacuum pump, high-precision pressure sensing device, and software control processing system, which can automatically control the extraction and replacement of gases, enabling the connected tanks to quickly reach anaerobic, microaerophilic, or oxygen concentration settings and carbon dioxide concentration settings in the cultivation environment. Then place the culture tank with the set gas environment in a constant temperature incubator for cultivation.

New generation anaerobic/microaerophilic cultivation methods:

→ Mode selection, one click start, complete manual operation in 10 seconds;

The fastest time to reach anaerobic environment is only 4 minutes, and the fastest time to reach microaerophilic environment is only 2 minutes;

Ready to use, no need to wait.

Level 5 Quality Control:

Product advantages:

1. Multi purpose: anaerobic, microaerophilic, and CO2 loving cultivation;

2. Rapid generation environment: microaerophilic for about 2 minutes, anaerobic for about 4 minutes;

3. Flexible tank configuration: Multiple tank options available to meet different cultivation needs;

4. QA quality control software: Cultivate the first 5 levels of quality control testing (gas input pressure detection, tank connectivity detection, tank leakage detection, tank sealing detection, catalyst activity detection) to ensure 100% reproducibility and eliminate speculation;

5. Parallel cultivation: Multiple tanks can simultaneously achieve cultivation in environments with different sample types, temperatures, and oxygen concentrations.

Flexible configuration of multiple matching tanks:

DW-100A-K series anaerobic microaerophilic cultivationTechnical Specifications:

1. Function: Mainly used to create anaerobic environments with oxygen concentrations of 0%, microaerophilic environments with oxygen concentrations of 6%, and special oxygen ratios of 1% -15%. Suitable for anaerobic bacteria, microaerophilic bacteria, and cell culture.

2. Working conditions and dimensions:

2.1 Working environment temperature: 10-35 ℃

2.2 Working environment humidity: 20-80%

2.3 Power supply: 220V ± 10%, 50 Hz ± 1

2.4 Dimensions (L × W × H): ≤ 335mm × 250mm × 330mm

3. The same system can simultaneously meet the gas environment preparation requirements for anaerobic and microaerophilic bacterial projects, and different project programs can achieve one click automatic switching; The project switching time is ≤ 10 seconds, and there is no need to replace the gas cylinder in between.

4. Applicable testing items: Used for the detection of Campylobacter jejuni, Streptococcus pyogenes, Bifidobacterium, Lactobacillus, and Shigella required by the national food safety standard GB4789; It can also be used for the isolation and cultivation of anaerobic, microaerophilic, and special oxygen concentration cultured bacteria such as Clostridium perfringens in natural mineral water for drinking.

5. Can quickly reach the cultivation conditions: "microaerophilic" conditions, with a fastest time of ≤ 2 minutes; Under anaerobic conditions, the fastest time is ≤ 4 minutes.

6. The built-in MCU realizes self checking of software system and built-in air valve hardware performance every time it is turned on, with a self checking time of ≤ 5 seconds.

7. Operation method:

7.1 Adopting a color operation screen of ≥ 4 inches, with built-in software supporting user menu based selection of cultivation projects.

The menu includes cultivation of curved bacteria, anaerobic cultivation, microaerophilic cultivation, and "special oxygen ratio gas cultivation environment generation".

7.3 Menu based selection, one click start, automatic generation.

8. Controller (host):

8.1 Includes vacuum pump, culture tank interface, gas cylinder interface, and programming control components.

8.2 Programming Control Components: All program parameters, such as vacuum pressure, reaction gas pressure, and the number of pumping and injection cycles, can be controlled through controller programming; After programming, the entire process of generating microaerophilic/anaerobic conditions runs automatically with one click startup, without the need for human intervention in between.

9. Gas consumption: can reach microaerophilic demand ≤ 2 L/12 per petri dish; Anaerobic ≤ 7L/12 petri dishes.

10. Multi functional and multi specification cultivation tanks are optional: the cultivation tanks are clear and transparent for easy observation, and each tank can support multiple cultivation applications, including anaerobic, microaerophilic, etc; Supports at least 6 specifications of culture tanks to choose from, including small culture tanks (with 6 ф 9cm culture plates placed in a single tank), medium culture tanks (with 12 ф 9cm culture plates placed in a single tank), medium double well culture tanks (with 24 ф 9cm culture plates placed in a single tank), curved bacteria culture tanks (with 8 curved bacteria culture double well culture plates placed in a single tank), specialized culture tanks for microbial identification (with 4 enzyme-linked immunosorbent assay plates/cell culture plates/identification strip culture plates placed in a single tank), large culture tanks (with 36 ф 9cm culture plates or four 250ml triangular bottles or 8 homogeneous bags placed in a single tank), etc.

11. The culture tank is equipped with a variety of supports: suitable for culture dishes, double hole culture dishes, microbial identification Flat noodles, triangular bottles and other microbial experimental carriers.

12. Can be connected to tanks: The microbial cultivation system can be matched with 1-100 tanks, each of which can provide different cultivation environments, and each tank can be independently controlled. There are ≥ 6 capacity options within the range of 1.5L-7.5L tank capacity.

13. Quality control and testing: Software QA quality control program, ≥ 5 level quality control: tank connection, input gas pressure, tank leakage, tank cover sealing, and anaerobic catalyst activity quality control.

14. Different quantities and sizes of culture tanks can be increased according to actual workload; Can add gas tank connections and perform corresponding software upgrades.

15. System configuration:

15.1 System host: 1 unit

15.2 System control components and quality control software: 1 set

15.3 Small culture tank and stainless steel culture dish fixture (can hold 6 dishes, ф 9cm): 1 set

15.4 Medium sized double hole culture tank and stainless steel culture dish fixture (can hold 24 dishes, ф 9cm): 1 set

15.5 Microbial identification specialized culture tank: 1 unit

15.6 Stainless steel culture fixture (can hold 4 enzyme-linked immunosorbent assay plates/cell culture plates/identification strip culture plates): 1 piece

15.7 Secondary gas pressure reducing valve: 1 unit

15.8 Palladium particle catalyst: 6 bags

15.9 Catalyst specific oven: 1 unit

15.10 Free 8L standard anaerobic mixture: 1 bottle