Ammonia analyzer (Biolytic)

一、 DxOligoAmmonia analyzer (Biolytic)Technical parameters:

Reactor capacity: 5.6 liters capacity;

Sample quantity: 1 to 5, each sample has 96 bits;

Temperature range: 120 ° C

Warranty: 12 months;

Power supply: 220V to 240V/50/60Hz;

Power consumption: 700 watts;

Weight: 30 kilograms;

2、 The product can be customized:

(1) Biolytic China provides a variety of instruments and accessories for oligonucleotide/DNA synthesis and oligonucleotide purification. Dr. Oligo series DNA synthesizer, Oligo processor, and Dr. OligoAmmonia analyzer (Biolytic)It is designed and manufactured in the United States to meet the rigorous requirements of any laboratory, research institute, or enterprise.

(2) All of our instruments and accessories are made of the latest materials and technologies to improve operational efficiency and reduce costs. Each instrument undergoes strict quality control procedures before leaving the factory. We take pride in providing high-quality customer service and support, and are committed to providing installation and training within the industry. All instruments and accessories can be customized according to your needs.

3、 Product advantages:

The DNA synthesis ammonia analyzer is designed for the effective preparation of biological samples, used for PCR primer, probe, and component production, DNA sequencing, DNA and RNA synthesis, random start, vector design, DNA fingerprinting, and artificial gene synthesis. It is a fast, efficient, and stable solution for deprotection of DNA/RNA/oligonucleotides.

4、 The comparison with traditional ammonolysis is as follows:

(1) By using the Oligo high-pressure ammonolysis analyzer, 480 samples can be ammonified at once and dried directly, greatly reducing the ammonolysis time.

(2) The traditional steps of ammonolysis are as follows, which are time-consuming and laborious. Check if the materials and reagents required for purification are sufficient. Activate the C18 plate (column) and fill it with 0.5M NaCl, drain it, repeat equilibration 3 times, then fill it with analytical grade, drain it more than 5 times, and centrifuge at the elution rate. After elution, fill the C18 plate with 0.5M NaCl, drain, repeat 3 times, and then fill the C18 plate with 0.5M NaCl for later use. C18 column activation, first activate C18 with 20ml analytical grade once, blow dry methanol, and then equilibrate with 10ml NaCl once for use.

(3) Calculate the number of pre used analysis plates and electrophoresis plates for plate gluing. For the production of adhesive solution, each board requires about 50ml of 16% adhesive (short chain, 16%; long chain, 12%). An average of 100ul of ammonium persulfate and 30ul of TEMED (depending on the situation, can be adjusted appropriately) are added to each board. Shake and mix well,Pour into the board and place horizontally to allow the glue to solidify. Be careful not to have bubbles in the board and pay attention to repairing the glue.

5、 Attention:

① The C18 board used requires regular maintenance and testing.

② Before using each new bottle of glue, it is necessary to mark the glue to ensure normal use.

③ Principle: Cut - chemically cut the synthesized oligonucleotide chain from the support. Use ice cold ammonia solution to crack CPG and connect the ester bond between the compound and the initial nucleoside. The broken oligonucleotide carries a free 3-hydroxy group. Deprotection - treating with ice ammonia solution for a longer period of time to remove the base ringProtective groups such as cyanoethyl, benzoyl, isopropyl, etc.

6、 Single tube ammonolysis principle:

(1) Use a syringe needle to inject CPG into the synthesized columnPour all the resin powder into the ammonia hydrolysis bottle and attach the corresponding label to the ammonia hydrolysis bottle.

(2) Add 100ul of ice triethylamine and 1.5ml of ice ammonia solution, cover the bottle tightly, and place it in an 80 degree oven for ammonia hydrolysis for 2 hours. The sequence synthesized by rapid ammonolysis of monomers can be directly added with ammonia water at 55 degrees Celsius for 2 hours. Accurately record the start time of ammonolysis on the production operation table.

(3) Attention: ① When placing primers into the ammonia hydrolysis bottle, check the labels clearly and match them one by one. ② The sequence of triethylamine and ammonia solution should be prevented from being added in reverse. (Adding the opposite will result in stratification). ③ The ammonia solution used in ammonolysis must be in a frozen state.

7、 2.96 Orifice plate ammonolysis principle:

(1) After 40 minutes of 90 degree ammonolysis, take it out and place it in a fume hood to drive ammonia for 1 minute, then centrifuge at high speed. Take the synthetic board and use a 20ul small gun to take a small sample of 20ul. Add 200ul of ice ammonia solution to each hole of the ammonia hydrolysis plate, seal the tape, clamp it with a clamp, record the time, and place it in an 80 degree oven for ammonia hydrolysis for 2 hours

(2) Attention:

① Be sure to attach the label of this round on the front of the ammonia hydrolysis plate, and then place corresponding pads under the synthesis plate one by one, and perform ammonia hydrolysis and centrifugation together.

② The sequence of triethylamine and ammonia solution should be prevented from being added in reverse. (Adding the opposite will result in stratification).

③ The ammonia solution used in ammonolysis must be in a frozen state.

④ When sealing the clamp, pay attention to tightening to prevent ammonia leakage.