ABI3500 sequencer

1ABI3500 sequencerOverview:

(1) Sanger sequencing is an accurate and simple method for genetic variation, while Applied Bisystems' gene analysis platform, as an industry standard, provides a reliable, efficient, and well documented DNA sequence analysis platform. The 3500Dx series gene analyzer can fully support the demanding performance requirements in clinical laboratories;

(2) The product has been certified by CEIVD and SFDA institutions and can be used for long fragment DNA sequencing, rapid resequencing, flexible fragment analysis, or a combination of multiple applications;

(3) Widely used in bacterial and viral genotyping and mutation identification, drug sensitive gene analysis, tumor gene mutation analysis, chromosome aneuploidy analysis, phylogenetic analysis, and genetic analysis of various genetic diseases (STR, SNP, MLPA, MI, LOH, Methylation, etc.).

IIABI3500 sequencerMain features:

1.8 channel 3500Dx capillary system

2. Certified by CEIVD and SFDA institutions, suitable for clinical testing and scientific research use

3. Wireless Radio Frequency Identification (RFID) technology tracks key consumable data and records management information

The 4.505nm single wavelength solid-state long-life laser source is powered by a standard power supply and does not require heat dissipation

5. Advanced multi-color fluorescence analysis capability, capable of multiple detection of up to 6 different fluorescent dyes on DNA fragments

6. Advanced thermal conductivity system design to better meet the strict temperature control requirements for DNA fragment analysis

7. The signal intensity between different instruments and between different operations, as well as between different capillaries, has been significantly improved

8. * Comprehensive data collection and preliminary analysis software provides real-time evaluation of data quality

9. Application flexibility: A capillary array and a polymer separation gel, commonly used in most research applications

10. Simple installation, operation, and maintenance of DNA sequencers that are easy to own and operate so far

3、 Detailed parameters:

1. Working conditions:

Power supply voltage: 100-240V ± 10%, 50/60Hz ± 10%

Current: 15A; power consumption 417VA, 371W (approximate value, excluding computer and monitor)

Environmental temperature: 15-30 ℃ (the fluctuation of room temperature during instrument operation should not exceed ± 2 ℃)

Humidity: 20-80%

2. Size:

Width (door closed): 61cm Width (door open): 122cm

Depth: 61cm

Height: 72cm

Weight: 82kg (approximate)

3. Laser and electrophoresis power supply:

Long lifespan, wavelength 505nm, solid-state laser excitation source

Electrophoretic voltage up to 20kV

4. Data detection host

Capillary electrophoresis system: 3500 gene analyzer with 8-channel capillary and 3500xL gene analyzer with 24 channel capillary;

Electrophoretic voltage: up to 20kV

Sample disk specifications: 96 well (regular plate or rapid plate) or 384 well sample disk, 8-tube (standard tube or rapid tube) can also be used

Capillary temperature control range: dynamic temperature control from 18 ℃ to 70 ℃

Operation mode: automatic glue filling, sample loading, electrophoresis analysis, detection and data analysis, without manual intervention

Advanced multi-color fluorescence analysis capability, real-time detection of four-color or five color fluorescence, and multiple detection of up to 6 different fluorescent dyes for DNA fragments

Laser light source: Long lifespan, wavelength 505nm, solid-state laser excitation source powered by standard power supply, no need for heat dissipation

The advanced design of the thermal conductivity system better meets the strict temperature control requirements for DNA fragment analysis

The signal intensity between different instruments, different operations, and different capillaries has been significantly improved

Wireless Radio Frequency Identification (RFID) technology tracks key consumables (capillaries, POP glue, cathode buffer, anode buffer) data and records management information

New version of data collection software:

New data collection software for preliminary analysis, with a concise user interface and clear design that can easily display information on the use of consumables and capillaries

Quick start function, system maintenance reminder, and guided sample board settings specifically designed for HID

Real time analysis and evaluation of data, rapid selection, and resampling functionality

And optional upgrade options provide features such as security, verification, and electronic signature

5. Control system (low requirement):

Hardware: Pentium IV1.86GHz processor

Operating System: Windows ® VistaSP1

Memory: 2GB

Hard drive: 1 x 80GB 7200RPM SATA 3.0GB/s and 8MB data cache

Note: Mainstream configuration upon delivery, and meet low requirements

6. Software

Data collection software

SequencingAnalysis software - used for analyzing, displaying, editing, maintaining, and printing sequencing data

Other optional secondary analysis software

Variant Reporter software - used for mutation detection and analysis, SNP discovery and validation, sequence confirmation

SeqScape software - used for mutation detection and allele identification based on sample libraries

GeneMapper software - an ideal tool for genotyping, allele determination, fragment analysis, and SNP analysis

Genemapper ® ID-Xv1.2 Software - Individual Recognition and Kinship Identification Software

7. DNA sequencing system

Measurement speed: The 3500 model can complete sequence detection of 8 samples within 40 minutes and can detect 280 samples in one day; The 3500xL model can complete sequence detection of 24 samples within 40 minutes and can detect 840 samples in one day

Sequencing length: Choose the appropriate gel, and the detection length can exceed 1000bp

SequencingAnalysis software - used for analyzing, displaying, editing, maintaining, and printing sequencing data

Variant Reporter software - used for mutation detection and analysis, SNP discovery and validation, sequence confirmation

SeqScape software - used for mutation detection and allele identification based on sample libraries

Multiple matching sequencing kits, including the universal sequencing analysis reagent BigDye ™ Terminatorv3.1、 Long fragment sequencing analysis reagent BigDye ™ Terminatorv1.1、 High GC sequencing analysis reagent dGTPBigDye ™ Terminatorv1.0、 One step sequencing analysis reagent BigDye ® DirectCycleSequencingKit

The MicroSEQ microbial identification system based on 16S ribosomal RNA gene sequencing can be matched, and the validated MicroSEQ database contains data on more than 2000 bacteria and 1000 fungi

Can be matched with human HLA typing system, including A, B, DR typing kits and related analysis software

Can be matched with human MitoSEQ ™ Mitochondrial whole genome sequencing reagents and analysis software

Can be equipped with sequencing based standard methods, test kits, and related software for detecting tumor gene mutations such as KRAS, BRAF, EGFR, etc., which comply with ASCO clinical standards

8. DNA fragment analysis system

The average running time for electrophoresis is 30 minutes, and the 3500xL model can process 1104 samples per day; The 3500 model can process 368 samples per day. (Note: Calculated based on working 23 hours per day)

Advanced multi-color fluorescence analysis capability, capable of multiplex detection of up to 6 different fluorescent dyes on DNA fragments

GeneMapper software - an ideal tool for genotyping, allele determination, fragment analysis, and SNP analysis

Genemapper ® ID-Xv1.2 is an automatic genotype analysis software designed specifically for human personal identification, including criminal investigation cases, DNA databases, and paternity testing. It optimizes the DNA data analysis process, improves analysis reliability and efficiency, and reduces analysis time

KRAS and BRAF gene mutation detection reagents based on multi base extension technology can be used to accurately quantify gene mutations in tumor tissues

Can be paired with BCR/ABL gene fusion detection related reagent kit analysis software

Aneuploidy assay kit and corresponding analysis software that can be used to identify aneuploid genetic defects such as trisomy 21 in placental villus tissue or amniotic fluid

Can be equipped with various virus detection kits TrueScience ™ RespiFinder ® Pannel， Up to 19 common respiratory viruses and bacteria can be detected in one reaction

It can be equipped with various forensic DNA individual identification kits such as Identififiler, Sinofiler, Yfiler, and Minifiler, which have been rigorously validated and can be used for ordinary cases, DNA databases, paternity testing, and donor stem cell survival analysis

9. System consumables

Capillary array

Internal uncoated capillary tube, 3500 model is assembled with 8 channels, 3500xL model is assembled with 24 channels

Featuring a fixed blue frame for easy installation

The capillary array is divided into two capillary lengths of 36cm and 50cm, supporting multiple applications and operating methods

Customization of 3500 and 3500xL capillary arrays (commitment) with 160 injections

Equipped with RFID tags, real-time monitoring of capillary usage information

Performance optimization of polymer POP-6 ™ And POP-7 ™ Glue

Designed as a plug and play bag that can be run by injection

There are two specifications of POP adhesive: 384 samples (60 more injections on the 3500 system; 20 more injections on the 3500xL system) and 960 samples (120 more injections on the 3500 system or 50 more injections on the 3500xL system)

Equipped with RFID tags, real-time monitoring of capillary usage information

Cathodic buffer tank (CBC)

Pre install 1 x buffer solution in the slot to support all electrophoresis applications

The cathode buffer tank is allowed to be used on the instrument for an additional 7 days after initial installation (or 120 more injections on the 3500 system, or 50 more injections on the 3500xL system, whichever comes first)

Equipped with RFID tags, real-time monitoring of capillary usage information

Anode buffer tank (ABC)

Pre install 1 x buffer solution in the tank to maintain the ion source and correct pH during electrophoresis

The anode buffer tank is allowed to be used on the instrument for an additional 7 days after initial installation (or 120 more injections on the 3500 system, or 50 more injections on the 3500xL system, whichever comes first)

Equipped with RFID tags, real-time monitoring of capillary usage information