5L double-layer glass fermentation tank

1、 Product Overview

5L double-layer glass fermentation tankspecially designed forOptimization of Microbial Fermentation ProcessDesign. Suitable for high-density cultivation of bacteria, yeast, and genetically engineered bacteria for metabolite synthesis.

2、 Core parameters

1. Tank system

• materialDouble layer high borosilicate glass tank body+ SUS316LStainless steel top cover/Interface (electrolytic polishing,Ra≤0.4μm）.

• volume: Total volume5LWorking volume2-4L(Liquid loading coefficient)70%）.

• design pressure：0.15-0.2MPa.

• Sterilization methodOffline sterilization of high-pressure sterilization pot.

2. mixing system

• Drive TypeTop mounted mechanical mixing (servo motor, stepless speed regulation).

• speed range：50-1000 RPM(Precision)±1 RPM）.

• stirring paddleDouble layered six blade turbine propeller (optional low shear blade).

3. process control

• temperature control：

• Scope: Environmental temperature+5℃~75℃（PIDAdjustment).

• Accuracy:±0.1℃, Resolution0.1℃.

• heat/Cooling: Electric heating film+Stainless steel jacket circulating water.

• pHcontrol：

• Scope:2-12, accuracy±0.01(Mettler)/Hamilton electrode).

• Linkage replenishment: acid/The alkali peristaltic pump is automatically adjusted.

• Dissolved oxygen（DO）Control：

• Scope:0-200%, accuracy±1%.

• Cascade regulation: Optimizing dissolved oxygen through three-level linkage of speed, ventilation, and feeding.

4. Ventilation and filtration

• ventilation volume：0.1-10 L/min(Rotary flowmeter, manual)/Automatic adjustment).

• filtration system：

• Air intake:0.22μmPTFE hydrophobic filter cartridge.

• Exhaust gas:0.22μmfilter+316LCondenser.

5. Supplementing and defoaming

• Feeding systemFour peristaltic pumps (acid, alkali, feeding, defoaming), supporting time proportional or feedback control.

• Defoaming controlCapacitive sensor+The peristaltic pump automatically adds defoamers and displays the accumulated amount.

3、 Feature Highlights

1. 5L double-layer glass fermentation tankoperation：

• Independent or linked control, supporting batch, flow addition, and continuous cultivation modes.

• Unified interface eliminates system errors and improves data comparability.

2. intelligent control system：

• industrial-gradePLC+10Inch touch screen (IoT function, supportsAppRemote monitoring).

• Real time data recording (temperaturepHTheDO, speed, etc.), supportUSBExport and cloud storage.

3. Modular expansion：

• Optional exhaust gas analysis（O₂/CO₂）Online biomass detection (turbidity), glucose concentration sensor, etc.

4. Safety and cleanliness：

• Corrosion resistant and easy to clean.

• Over limit alarm and self-locking function to prevent equipment damage caused by misoperation.

4、 Technical Configuration Checklist

5、 Application scenarios

• Process Development: Selection of culture medium formula, dissolved oxygen/Optimization of replenishment strategy.

• Strain validationScreening of high-yield strains and stability testing of genetically engineered bacteria.

• Teaching and ResearchDemonstration of microbial cultivation and metabolic flux analysis in university laboratories.

6、 Precautions

• Ventilation pressureInput gas pressure required≤0.2MPaTo avoid overpressure in the tank.

• Sterilization compatibilityGlass tanks only support offline high-pressure sterilization（121℃/30min）.