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Shanghai Alpha Meijia Biotechnology Co., Ltd
New digital flow cytometry imaging system
NegotiableUpdate on 07/10
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Overview
FlowCam 8400 is an insoluble particle detection system specially optimized for the pharmaceutical industry. It integrates digital imaging, microscopic magnification, and particle counting into an automatic instrument for analyzing suspended particles in liquids. When trace samples flow through the imaging area of the flow cell, the instrument automatically captures particle images. By using specialized software to analyze each particle, statistically valuable data such as particle size distribution, morphology distribution, and particle concentration can be obtained.
Product Details
FlowCam 8400 is an insoluble particle detection system specially optimized for the pharmaceutical industry. It integrates digital imaging, microscopic magnification, and particle counting into an automatic instrument for analyzing suspended particles in liquids. When trace samples flow through the imaging area of the flow cell, the instrument automatically captures particle images. By using specialized software to analyze each particle, statistically valuable data such as particle size distribution, morphology distribution, and particle concentration can be obtained.
Particle detection range: 2.0 μ m-1000 μ m
Magnification: Standard 10X objective lens
Sample dosage: minimum 100ul
Sample processing capacity: 50-5000ul/min, stepless speed regulation
Effective sample rate: ≥ 85%
Study on insoluble particles in blood preparations
Research on Aggregates and Particles in Vaccine Development
Study on the content of agglomerates in suspension formulations
Introduction to the entire Flowcam testing process
1. Manual/automatic sample addition
The high-precision injection pump sucks the sample into the flow cell. During this period, after the sample flows through the liquid sensor, the software can start data collection.
2. Particle image acquisition
High speed cameras capture full width and full depth images of the flow pool;
The various images of particles are segmented and extracted in real-time from camera images.
3. Data analysis
The supporting software will display particle number, particle concentration, particle size and distribution, as well as particle morphology parameters. The results can be output in any form. For example, size histograms, CSV, etc.
FlowCam, also known as dynamic particle real-time imaging method, is used for imaging analysis of dynamic particles;
Sample non-destructive, high particle image resolution, high-throughput;
One experiment can obtain particle images, size and distribution, particle number, particle concentration, and morphological parameter information.
Features
FlowCamIt can automatically capture microscopic images of microorganisms and particles in liquids and quickly analyze them.
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1. FlowCamIt is a * tool used to detect the concentration, size, and source of particles in various industries; 2. FlowCamIt is very valuable in the development and quality control stages of biological therapy; 3. Utilizing high-quality imaging to characterize particle properties and assist in product development and quality control across various industries; 4. Can be used as an orthogonal method necessary for other particle characterization methods; 5. Compared with traditional detection methods, it has higher production efficiency and lower cost; 6. Fully automated and AI intelligent development to support industry development; |
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7. Mirror images and rapid analysis; 8. Automated the tedious manual observation process; 9. High speed shooting of a large number of particles (tens of thousands per minute); 10. Real time measurement and analysis of particle information; 11. A multifunctional device that integrates a microscope, particle size analyzer, and flow cytometer; 12. Powder samples can be dissolved in solvents for observation. |
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Detection Principle
The FlowCam particle flow imaging analyzer, also known as the dynamic particle image analyzer, works by real-time and dynamic imaging analysis of sub visible and visible level particles in the solution flowing through the flow cell.
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1. Manually or automatically add the sample into the injection port; 2. The high-precision injection pump sucks the sample into the flow cell, and the fluid sensor starts data collection; When the sample flows through the optical field of view, the high-speed camera will record the full width and full depth images of the flow cell; 4. The camera captures panoramic images of particles flowing through the flow pool in real time, and segments and records the particle images in real-time; 5. The data can be further analyzed, grouped, and filtered after collection. |
technical advantages
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The photoresist method automatically counts the number of insoluble particles of different sizes in the sample through the principle of photoelectric conversion. However, the information obtained by this method is limited and cannot distinguish the properties of the measured particles, nor can it analyze and control their causes during the research and development stage. The microscope method also has drawbacks such as low efficiency and easy counting errors caused by human eye fatigue. FlowCam 8100 can capture particles in a dynamic flow cell through an electronic imaging system, and then analyze the size, shape, refractive index, and other parameters of insoluble particles through software to distinguish all images. |
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01 Provide high-throughput, high accuracy, and high precision compared to traditional particle analysis
02 expandable fluorescence laser module, capable of identifying specific particles;
03 High imaging quality, suitable for characterizing particles in the sub micron to visible size range, with accurate and statistically significant results;
Low sample usage, easy operation, and expandable automation system.
Software Advantages
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VisualSpreadsheet software VisualSpreadsheet is an integrated software program with * functions, which can set methods, obtain data, and process images captured by FlowCam. Analyze, sort, and filter images based on over 40 morphological parameters and their combinations Grouping and classification, or using the "search" function in the software to identify components in the sample. Grouping data from multiple runs or samples for comparison purposes.
VisualSpreadsheet provides optional software packages that comply with the 21CFR Part 11 standard. |
VisualAI ™ VisualAl is an optional artificial intelligence module. It is an instrument and sample independent tool that can automatically classify particles in biological therapeutic agents. Using the convenient VisualSpreadsheet 6 software, protein aggregates and silicone oil droplets can be identified and distinguished from other pollutants. |
Measure the characteristic quantities of various particles to achieve highly flexible data processing.
FlowCamThe pre installed analysis software can classify and filter data based on particle measurement results, and display them in a comprehensive manner.
Application Cases
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Various insoluble particles API aggregate particles Silicone oil droplets, glass fragments, degradation materials, plasticizers, etc Fiber and bacterial contamination Cell count, activity, and morphology |
Monitoring of harmful algal blooms HABs: identification of blue-green algae, diatoms, and other algae Optimization of Beneficial Algae Cultivation Process Microbial identification and monitoring in freshwater, seawater, rainwater, and drinking water Classification, identification, and monitoring of microplastics |
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Cleanliness control of electronic devices CMP chemical mechanical polishing solution Uniformity, color, and appearance of printer toner particles Evaluation of Cement Material Performance Ceramic Forming Process Battery conductivity performance |
Food flavor: viscosity Yeast activity: Beer brewing Pollen seeds: activity recognition Shelf life: Identification of pollutants |
Biopharmaceutical R&D
According to USP<1788>, the flow imaging particle analysis system uses the recommended method orthogonal to the photoresist method to determine the content of sub visible particles. FlowCam is widely used in biological therapy research, formulation, and development of protein, nanomedicine delivery systems, as well as cell and gene therapy products.
material characterization
FlowCam is used for a variety of applications focused on materials and chemicals, ranging from characterization of food and beverage ingredients to printer toner and superabrasive materials, ion exchange resins, chromatography column fillers, fibers, additive manufacturing, polymer composition analysis, chemicals, cosmetic formulations, and microencapsulation processes.
Marine and Freshwater Research
For over 20 years, scientists have used the automated, fast, accurate, and easy-to-use FlowCam as an alternative to manual microscopes to monitor plankton community composition. FlowCam has become an important product for studying marine and freshwater microorganisms.
Water quality monitoring and environment
FlowCam provides an active, cost-effective, and scalable solution for monitoring raw and purified water. Multiple water companies around the world use FlowCam to detect and quantify the quantity of taste and odor products, filter fouling diatoms, and potentially toxic blue-green algae. Environmental applications include monitoring soil microorganisms, pollen, seeds, and pollutants.
Biopharmaceutical R&D
Detection and measurement of cells, API aggregates, and other particles
Formula Development
QC diagnosis and batch issuance testing
Stability study and shelf life estimation
Purification process development
Characterization of excipients and API
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| Protein aggregates |
Silicone oil droplets |
Cell aggregates |
material characterization
abrasive
Wood and pulp fibers
Cosmetics and perfume
Food and Beverage
Microencapsulation
Oil and gas
Paint and polymers
Printer toner
Washing water
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| Color printer toner particles | polystyrene bead | Microcapsule flavor granules |
Marine and Freshwater Research
Identify and calculate phytoplankton and zooplankton
Investigating harmful algal blooms (HAB)
Supporting the Health of Aquaculture Systems
Observing microalgae cultivation
Analyze sediment particles
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| Marine plankton | Red tide algae (harmful algal blooms) | Freshwater plankton |
Water quality monitoring and environment
Identify blue-green algae, taste and odor, and other harmful algae in drinking water sources
Using data to provide information for water treatment decisions
Monitor filter performance
Detecting and monitoring soil microorganisms, mites, forest litter invertebrates, and nematodes
Determine seed vitality
Analyze pollen particles
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| Microcystis (Cyanobacteria) | Long spore algae (blue-green algae) | pollen |
instrument parameters
| Particle size range | 2μm~1mm |
| Magnification and Flow Pool |
20X(~200XMagnification), Flow Cell: 50 μ m FOV 10X(~100XMagnification), Flow Cell: 80 μ m FOV or 100 μ m FOV 4X (~40X magnification), flow cell: 300 μ m FOV or 600 μ m FOV 2X (~20X magnification), flow cell: 1mm FOV |
| Minimum sample size | 100 μL |
| Sample processing capability | At 20X, 0.05mL/minute; At 2X, maximum 10mL/minute |
| fluid | Multiple sizes of micro injection pumps can optimize flow rates: 0.5mL, 1mL, 2.5 mL, 5mL, 12.5 mL |
| pump flow rate | 0.02 mL/min~10 mL/min |
| Maximum particle concentration | When the particle size is 2.5 μ m, it is 5 million particles/mL |
| Data collection method |
FlowCam 8100: Automatic Imaging; FlowCam Cyano and FlowCam 8400: Fluorescence based Laser Triggering and Automatic Imaging |
| camera | High resolution (1920 × 1200 pixels) CMOS. Available in monochrome and color |
| frame rate | Shutter speed up to 100 frames per second |
| Focusing method | automatic |
| measurement parameters |
Basic shape parameters:Area, aspect ratio (width/length), diameter (spherical and based on area), length, volume (based on ABD), volume (based on ESD), width, and three biological volume measurements Advanced morphological parameters:Area (filling), circle fitting, roundness, roundness (Hu), tightness, convex perimeter, convexity, elongation, fiber curvature, fiber straightness, geodesic aspect ratio, geodesic length, geodesic thickness, perimeter, roughness, symmetry Grayscale and color measurement:Average blue, average green, average red, blue/green ratio, red/blue ratio, red/green ratio, edge gradient, intensity, Sigma intensity, total intensity, transparency |
| automation | ALH automatic liquid processor compatible with FlowCam |
| Software regulatory compliance | Provide 21 CFR Part 11 options |
| Dimensions and Weight | 36 cm (width) x 43 cm (depth) x 38 cm (height), 27 kg (43 kg: factory weight) |
| Power requirements |
FlowCam 8400: Maximum 92W; FlowCam Cyano and FlowCam 8400: Maximum 115W |
Configuration Selection
| Model (Host) | General | 配置 Specification |
| FlowCam 8400 (532 nm) |
Host:Flow imaging microscope host for aquatic applications Equipped with high-resolution CMOS color camera and autofocus function, achieving fast data acquisition. Matching objective lens kit: objective lense:2X, 4X, 10X, and 20X, equipped with matching high-quality optical flow cells and precision volume injectors, can cover a wide size range of 2 μ m-1 mm. (The specific testing scope is subject to the selected kit specifications) Software:Including VisualSpreadsheet software for data collection and comprehensive image analysis. Warranty:The factory provides a one-year standard warranty. For detailed information, please refer to the terms and conditions. |
It has 532 nm laser excitation and distinguishes organisms through fluorescence triggering. Used for aquatic applications. The available fluorescence detection channels include 650 nm LP (such as chlorophyll) and 575/30 (such as phycoerythrin). |
| FlowCam 8400 （532 nm） - ALH 兼容 |
It has 532 nm laser excitation and distinguishes organisms through fluorescence triggering. Used for aquatic applications. The available fluorescence detection channels include 650 nm LP (such as chlorophyll) and 575/30 (such as phycoerythrin). ALH for FlowCam is pre configured for automatic liquid handling. |
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| FlowCam 8400 (488 nm) |
It has 488 nm laser excitation and can distinguish organisms through fluorescence triggering. The available fluorescence detection channels include 650 nm LP (such as chlorophyll) and Ch2 525/30 BP and Ch2 525/30 BP (such as FITC). |
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| FlowCam 8400 （488nm）- ALH 兼容 |
It has 488 nm laser excitation and can distinguish organisms through fluorescence triggering. The available fluorescence detection channels include 650 nm LP (such as chlorophyll) and Ch2 525/30 BP (such as FITC). Pre configured automatic liquid handling and ALH for FlowCam. |
