Perfusable microvascular system

Perfusable microvascular system

Although 3D organ models provide complexity and structural maturity, their lack of angiogenesis and perfusion limits their ability to effectively summarize organ physiology. Designed a microfluidic platform called IFlowPlate, which can be used for in vitro culture

Up to 128 colonic organoids with standing perfusion and vascularization. Unlike traditional microfluidic devices, the vascularized organ chip device with an "open-air" design does not require any external pumping system and allows for downstream analysis of tissue extraction, such as tissue chemistry

Even internal transplantation. By optimizing the formulation of extracellular matrix (ECM) and culture medium, patient derived colon organs were successfully cultured in a self-assembled vascular network, and it was found that under constant perfusion, the colon machine could grow better on the platform compared to traditional static methods

Compared to the state conditions. In addition, a colitis model with innate rabbit plague function was developed using this platform, in which circulating monocytes can be recruited from the vascular system, differentiated into macrophages, and infiltrated into colonic organoids in response to tumor necrosis factor (TNF)-

Stimulation of inflammatory cytokines. With the ability to grow vascularized colonic organoids under intravascular perfusion, the IFlowPlate platform can bring new possibilities for screening potential therapeutic targets or modeling related diseases.

Features:

1. Perfusable microvascular network

Experience next-generation vascular research through our self-assembled microvascular network.

The main characteristics of self-assembled microvascular networks are:

Self assembling microvascular system: Utilizing the power of self-assembly to construct a seamless and highly accurate microvascular network, providing unparalleled biological accuracy and research potential.

Untamed membrane formation: eliminates the need for synthetic membranes, providing a more realistic and representative environment for studying vascular physiology and interactions.

Perfuse and easy access to the lumen: Experience easy access to the lumen chamber, achieve efficient perfusion, and facilitate various experimental procedures and evaluations.

Support for circulating immune cell delivery: Our network supports the delivery of circulating immune cells, enabling researchers to study rabbit epidemic response and vascular interactions with unprecedented ease and ease.

High throughput imaging: Utilize our high-throughput imaging capabilities for real-time, high-resolution visualization and analysis of microvascular dynamics, ensuring faster and more accurate results.

Self assembling microvascular networks are a promising solution for researchers, clinical doctors, and pharmaceutical companies seeking to deepen their understanding of vascular biology, develop innovative treatment methods, and unleash the potential of microvascular research.

2. Colonic organoids with perfusable vascular system

2. Colonic organoids with a submersible vascular system

Using Iflowplate? Discovering the future of organ research, this is our innovative microfluidic platform aimed at overcoming the limitations of traditional 3D organ models. IFlowPlate? Capable of cultivating up to 128 colonic organoids with standing perfusion and vascularization in vitro,

Thus, it can more accurately represent organ physiology.

Using Iflowplate? Discovering the future of organ research, this is our innovative microfluidic platform aimed at overcoming the limitations of traditional 3D organ models. IFlowPlate? Capable of cultivating up to 128 colonic organoids with standing perfusion and vascularization in vitro,

Thus, it can more accurately represent organ physiology.

IFlowPlateTM

Unlike traditional microfluidic devices, our unique "open" design eliminates the need for external pump systems and allows for easy tissue extraction for downstream analysis, such as tissue chemistry or in vivo transplantation.

By optimizing the extracellular matrix (ECM) and culture medium formula, IFlowPlate? Successfully co cultured patient derived colon organoids within a self-assembled vascular network. Compared to traditional static conditions, our platform has been proven to significantly improve organ growth.

IFlowPlate? Congenital immune function can also be used to model colitis. Circulating monocytes can be recruited from the vascular system, differentiate into macrophages, and infiltrate colonic organoids in response to stimulation by TNF-a inflammatory cytokines.

Open hole design for easy organization, extraction, and downstream analysis

Optimize ECIM and culture medium formula to improve organoid growth

Effective modeling of colitis and innate immune function

IFlowPlate? Seeking the ultimate solution for researchers, pharmaceutical companies, and clinical doctors to advance research and development of organoids.

BioColonix Introduction: A Breakthrough In Vitro Colonic Model for IBD Research and Treatment Development

BioColonix is an in vitro colon model designed to investigate changes in inflammatory bowel disease (IBD) and accelerate the development of innovative therapies.

Our technology overcomes the limitations of current colon models by integrating vascularized crypts, which can accurately replicate the physiological conditions of both healthy and diseased colon.

Our proprietary IFlowPlate384 platform enables us to create highly realistic models of the colonic epithelial barrier. We imprint the crypt topography onto the scaffold and then fill the scaffold with colon cells.

The proliferating colon cells spontaneously localize to the crypt microenvironment. By integrating a perfusable microvascular system around the colonic crypts, BioColonix can simulate the cytokine gradient observed in tissues, similar to the basal to apical matrix in vivo

The main features of BioColonix are:

Copy vascularized crypts of colon physiological conditions

The IFlowPlate384 platform can achieve crypt topography and colon cell populations

Accurately simulate colonic physiology and disease models

In vivo matrix substrate to apical cytokine gradient and inflammatory response

BioColonix is the ultimate solution for researchers, clinicians, and pharmaceutical companies aimed at better understanding IBD and developing effective treatment methods.