Stiffness adjustable microstructure gel

The matrix for cultivating cells contains soft or rigid microstructures in the form of open microchannels or pores.Therefore, cells can be confined to the 3D environment or on top of substrates containing this terrain feature.

Many geometric shapes of microstructures are possible (channel/pore sizes ranging from um to mm)

The stiffness of the substrate can be selected from hard (1 MPa) to soft (<500 Pa)

Quick to use, as cells are directly seeded at the top of the features (facilitating restriction of non migrating cells)

Biological functionalization of ECM proteins

Suitable for any cell culture medium (from culture dish to 96 well plate)

Compatible with high-resolution optical microscope systems

application

Cell differentiation, cell mechanics, 3D cell shape control, cell migration, tissue geometric mimicry, etc

Fibroblasts on micro column matrix

Standard cultivation restrictions

In standard culture, cardiomyocytes derived from pluripotent stem cells have a random shape and no tissue, thus exhibiting immature characteristics.

Cell differentiation assay

Cardiomyocytes differentiate in the matrix through topographical cues, as grooved surfaces exhibit natural arrangement and nuclear elongation.

example

Differences in cell and nucleus extension of cloned cardiac stem cells on microgroove matrix compared to conventional plate analysis.

Microchannel application