Multi fluorescence immunohistochemical detection

Multi fluorescence immunohistochemical detection（mIHC）：

The main technical principle comes from TSA technology, which is a type of enzymatic detection method that uses horseradish peroxidase (HRP) to perform high-density in situ labeling of target proteins or nucleic acids.

Multi fluorescence immunohistochemical detectionTechnology can be used with high-performance dye Alexa Fluor ®、 Traditional fluorescent dyes are compatible with colorimetric detection systems. The main principle of TSA is to utilize the peroxidase reaction of tyramine Tyramide (tyramine salt forms covalent binding sites under HRP catalyzed H202), producing a large amount of enzymatic products that can bind to surrounding protein residues. This results in a significant accumulation at the antigen antibody binding site, which then binds to Streptavidin HRP/fluorescent groups. After several cycles of amplification, a large number of enzyme molecules or fluorescent groups can be bound, leading to geometric amplification of the detection signal.

Multi fluorescence immunohistochemical detectionAdvantage:
1. High research value: divided into two series: human and mouse, convenient for parallel experiments on mouse tumor models and human samples for translational medicine research;

2. High compatibility: Fluorescent dye labeling is suitable for all microscopes, confocal microscopes, and fluorescence section scanning equipment equipped with fluorescence bands such as UV, FITC, CY3, CY5, etc;

3. Stability: There is no need to avoid light during the sample addition process. After staining, the sample is stored at 4 degrees Celsius for six months and at -20 ℃ for one year. The fluorescence still meets the requirements for photography;

4. Convenience: All antibodies have been screened, with good specificity and optimized staining concentration, reducing the need for researchers to manually determine the conditions.