Soil Lignin Peroxidase (SLiP) Test Kit

Product Details:
Soil lignin peroxidase(SLiP) Test BoxMeasurement significance

Lignin peroxidase(EC1.11.1.14) is a peroxidase containing heme, belonging to the lignin degrading enzyme system. It has great potential for applications in lignin biodegradation, papermaking industry, textile industry, aromatic compound conversion and degradation, and environmental pollution control.

Measurement principle

Lignin peroxidase oxidizes resveratrol to produce veratrol aldehyde, which is produced inThere is a characteristic absorption peak at 310nm.

Bring your own experimental supplies and instruments

Balance, low-temperature centrifuge, UV spectrophotometer/ELISA reader, trace quartz colorimetric dish/96 well plate (UV plate).
Reagent composition and preparation:

Reagent 1: Liquid1 bottle, stored at 4 ℃.

Reagent 2: Liquid1 bottle, stored at 4 ℃.

Reagent 3: Powder1 bottle (brown), stored at 4 ℃ in the dark. Add 667 μ L anhydrous ethanol before use, cover tightly and mix well. Then add 9.333 mL distilled water and mix well. Store in the dark.

Reagent 4: Powder1 tube, stored in the dark at 4 ℃. Add 1 mL of distilled water before use and dissolve thoroughly.

Standard product: Powder1 bottle, add 10 mL of distilled water before use, fully dissolve. 1 μ mol/mL standard solution, stored at 4 ℃ in the dark.
Product attributes:

Measurement operation:

Preheat the spectrophotometer/ELISA reader for 30 minutes, adjust the wavelength to 570 nm, and zero the distilled water.

2. Blank tube: Take an EP tube and add 10 μ L of distilled water, 100 μ L of reagent 2, 100 μ L of reagent 3, and 10 μ L of reagent 4. Mix well and cover tightly with a bottle cap (to prevent water loss). Place the tube in a boiling water bath for 15 minutes. After cooling, invert the EP tube several times and measure the absorbance value at 570nm, which is recorded as an empty white tube A. After color development, it is necessary to complete the measurement within 30 minutes.

3. Standard tube: Take an EP tube and add 10 μ L of standard substance, 100 μ L of reagent 2, 100 μ L of reagent 3, and 10 μ L of reagent 4. Mix well and cover tightly with a bottle cap (to prevent moisture loss). Place the tube in a boiling water bath for 15 minutes. After cooling, invert the EP tube several times and measure the absorbance value at 570nm, which is recorded as the A standard tube. After color development, it is necessary to complete the measurement within 30 minutes.

4. Measurement tube: Take an EP tube, add 10 μ L of supernatant, 100 μ L of reagent 2, 100 μ L of reagent 3, and 10 μ L of reagent 4, mix well, and cover tightly with a bottle cap (to prevent water loss). Place in a boiling water bath for 15 minutes, cool down, and repeatedly invert the EP tube several times. Measure the absorbance value at 570nm and record it as the A measurement tube. After color development, it is necessary to complete the measurement within 30 minutes.

Note: Blank tubes and standard tubes only need to be measured once.

The products currently being sold by the company:

Legionella pneumophila antigenELISA kit LP Ag free test agent

Retinoblastoma inhibitory proteinELISA kit pRB free test agent

Inducing genes/RIG-1 like receptor ELISA kit RLR free replacement test agent

Growth related proteins43 ELISA Kit GAP-43 Free Replacement Test Agent

Growth regulating oncogenesGamma/Melanoma Stimulating Factor ELISA Kit GRO Gamma/CXCL3/MGSA Free Replacement Test Agent

Growth regulating oncogenesβ/Melanoma Stimulating Factor ELISA Kit GRO β/CXCL2/MGSA Free Replacement Test Agent

Growth regulating oncogenesAlpha/Melanoma Stimulating Factor ELISA Kit GRO Alpha/CXCL1/MGSA Free Replacement Test Agent

Growth hormone releasing factorELISA kit GH-RF free test agent

Growth hormone releasing peptideELISA Kit Ghrelin Free Replacement Test Agent

Growth hormone binding proteinELISA kit GHBP free testing agent

Growth differentiation factor-9ELISA Kit GDF-9 Free Replacement Test Agent

Survival factorELISA kit, free substitute test agent for survivorin

Glycation end products of glomerular tissueELISA kit GTE-AGE free test agent

Adrenomedullin precursorELISA Kit Pro ADM Free Test Agent

Adrenal cortex antibodyELISA kit ACA free test agent
Soil lignin peroxidase(SLiP) Test BoxAcid phosphatase（ACP test box 100 tubes/48 samples micro method

Acid phosphatase（ACP test box 50 tubes/24 samples visible spectrophotometry method

Alkaline phosphatase（AKP/ALP) test box 100 tubes/48 samples micro method

Alkaline phosphatase（AKP/ALP test box 50 tubes/24 samples visible spectrophotometry method

Carboxylesterase（CarE) Test box 100 tubes/96 sample micro method

Carboxylesterase（CarE) Test box 50 tubes/48 samples visible spectrophotometry method

ATP content test box 100 tubes/48 samples micro method

ATP content test box 50 tubes/24 samples visible spectrophotometry method

ATP content test kit 50 tubes/48 samples HPLC method