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Rat cytokine detection reagent (flow cytometry)

NegotiableUpdate on 05/06
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Overview

Product Name: Beadstar mPlex #174; Rat Cytokine Combined Detection Kit $r $n Product Code: BS-RC-01 $r $n Specification: 100 T $r $n Detection Factors: IL-1 β, IL-2, IL-4, IL-5, IL-6, MCP-1, TNF - α (freely combinable) $r $n The kit is based on flow cytometry technology and can simultaneously and quantitatively detect 7 key inflammatory and immune regulatory factors in rat serum, cell culture supernatant, and tissue homogenate in a single reaction system. Only 25 μ L of sample is needed, and the detection can be completed within 3-4 hours.

Product Details


Product Name: Beadstar mPlex ® Rat multi cytokine combined detection kit

Item number: BS-RC-01

Specification: 100 T/50T

Detection factors: IL-1 β, IL-2, IL-4, IL-5, IL-6, MCP-1, TNF - α (freely combinable)


🔍 Four core advantages, reconstructing the "efficiency, accuracy, and flexibility" of multi factor detection

1. "Micro sample friendly": 25 μ L sample, full analysis of 7 factors

In response to the pain points of "difficult sample acquisition and small quantity" in animal experiments, this kit is based on flow cytometry (principle shown in Figure 1) and only requires 25 μ L of rat serum, cell supernatant, or more valuable cerebrospinal fluid samples to simultaneously achieve accurate quantification of IL-2, IL-4, IL-6, IL-1 β, IL-5, TNF - α, MCP-1.

大鼠7细胞因子检测试剂(流式荧光法)

Figure 1 Multi detection principle based on flow microsphere matrix

2. "Multi factor Joint Inspection" Efficiency Revolution: One Experiment=7 Data

Based on the independent research and development of BixinBeadstar-mPlex ® Fluorescence encoded microsphere technologyThe reagent kit can simultaneously detect 7 cytokines in the same micro sample. Taking the study of rat asthma model as an example, only one detection is needed to simultaneously capture the synergistic changes of multiple groups of cells, which improves the dilemma of "few samples and slow experiments".

3. "Customized testing plan": on-demand matching, precise focus on research objectives

Bixin Biotechnology is well aware of the diversity of research needs. You may focus on the study of "inflammatory factor clusters (IL-1 β, TNF - α, IL-6)", or you may need synchronized data on "immune regulatory factor groups (IL-2, IL-4, IL-5)". Therefore, we introduceCustomized testing planYou can flexibly combine cytokine combinations according to research purposes, Beadstar-mPlex ® The reagent kit will provide you with a personalized testing panel. Whether focusing on a single category factor or covering a multifunctional factor network, it can be accurately adapted, greatly improving research efficiency and saving time and cost.

4. 'Research grade precision': R ² ≥ 99.9%, even trace samples can produce 'hard data'

From the standard curve, it can be seen that the fit of each factorAll over 99.9%The detection sensitivity reaches PG level. Even low abundance cytokines in trace samples can be accurately captured; The quantitative results of highly expressed cytokines are also stable and reliable.


大鼠7细胞因子检测试剂(流式荧光法)



🚀 Application scenario: Even trace samples can cover the entire research chain

Rare sample research

As for cerebrospinal fluid samples from rats with spinal cord injury, only 25 μ L is needed to synchronously analyze the regulation of inflammatory factors such as IL-1 β and TNF - α on nerve injury, avoiding the regret of "sample depletion but insufficient data".
  • Longitudinal study tracking

    Multiple blood samples were collected from the same rat (only a small amount was taken each time) to monitor the dynamic changes of immune factors such as IL-2 and IL-4 for a long time, in order to meet the needs of "individual level mechanism research".
  • Micro evaluation of drug development

    In the initial screening stage of rat efficacy, a small sample is used to synchronously evaluate the regulatory effect of drugs on multiple cytokines, accelerating the judgment from "candidate compounds" to "drug potential".