In the field of biological sample preservation, the precise control of cell cryopreservation often determines the success or failure of scientific research and clinical efficacy. Traditional cooling methods are difficult to accurately control the temperature curve, resulting in cells being susceptible to ice crystal damage and significantly reduced activity during the freezing process. The program cooling device launched by CBS Corporation in the United States achieves precise temperature control from+50 ℃ to -180 ℃ through direct temperature feedback control technology, providing a reliable freezing protection solution for biological samples.

The CBS program cooling instrument adopts a direct temperature feedback control method, which does not need to consider the number and time of valve openings, but obtains accurate temperature control by injecting the amount of gaseous liquid nitrogen. The core lies in the precise coordination between the microprocessor control system and the solenoid valve, which precisely controls the cooling rate by controlling the amount of liquid nitrogen injected into the chamber. When the equipment is working, temperature detection is completed by two monitoring systems - the internal detection system of the cavity and the external detection system of the product. This dual monitoring system ensures the accuracy of temperature control. The thermocouple temperature probe equipped in the system can monitor the sample temperature in real time, and once an abnormality occurs, it will immediately trigger the alarm function. The temperature control range of the CBS program cooling instrument is extremely wide, from+50 ℃ to -180 ℃. The cooling rate can be accurately adjusted between 0.01 ℃ and 99 ℃ per minute, and the maximum heating rate is 20 ℃/minute. This wide range and high-precision temperature control capability enables it to meet the freezing needs of the vast majority of biological samples.

The CBS program cooling instrument is mainly divided into two series: the 2101 series laboratory type and the large capacity industrial type. The 2101 series has a capacity of 28 liters, with external dimensions of 648 millimeters x 743 millimeters x 484 millimeters (depth x height x width) and an effective internal space of 243 millimeters x 356 millimeters x 349 millimeters. This series has a power of 230V, 50/60HZ, temperature uniformity of 0.1 ℃, and temperature accuracy of 0.1 ℃. The running time of the equipment can range from 1 second to 99 hours, meeting experimental needs of varying lengths. The large capacity industrial type is a program cooling device specially designed by CBS for biopharmaceutical factories. Its freezer size can be customized according to user needs, suitable for large-scale production needs. Despite the increased capacity, its controller is the same as the laboratory model, using a desktop or laptop computer with a consistent operating interface. In terms of sample support, multiple freezing racks can be equipped, including 1.2ml/2ml, 4ml/5ml freezing tube racks, as well as various cell bag and blood bag freezing racks such as 50ml, 250ml, 500ml, etc. At the same time, it can process 650 1.2ml/2ml cryovials, 390 4ml/5ml cryovials, or 40 50ml blood bags, with considerable processing capacity.

The CBS program cooling device is equipped with control software based on the Windows operating system, providing an intuitive user interface and powerful programming capabilities. The software supports unlimited programming capabilities, allowing users to flexibly set complex temperature curves to meet the special needs of different samples. The system has multi-level password protection function, which ensures the security of programs and stored data through password settings of different security levels. Each running program contains 30 stored parameters, of which 27 are retrievable parameters and 3 are remark parameters, making it easy for users to record and analyze the freezing process in detail. The equipment and software comply with the requirements of 21 CFR part 11 regulations and have comprehensive data traceability capabilities. The system can automatically record the cooling curve of each freezing operation and store it on the hard drive or CD for easy subsequent query and analysis. Users can print frozen operation graphics and data through any standard USB printer, and also output data in CSV format for further analysis and report generation. The software also provides a searchable database of frozen operation history, helping users quickly locate past frozen programs.

Its high-precision temperature control can effectively ensure the safety and controllability of cryopreservation, and temperature control according to the program can ensure the consistency of the recovery quality of each batch of samples. The equipment is of all stainless steel structure, with 304 polished stainless steel liner, and attached with high-density expansion polyurethane foam insulation material to form an insulated chamber, effectively blocking the cold and heat exchange inside and outside the box. This design not only improves cooling efficiency, but also reduces liquid nitrogen consumption, making it more economical and environmentally friendly compared to traditional methods. Its flexibility is also commendable. Six preset standard cooling programs help operators quickly get started, while also supporting users to customize cooling curves according to special needs. All operational functions are equipped with manual override devices, which can be manually intervened when necessary. In terms of safety, the equipment is equipped with functions such as thermocouple temperature probe error alarm, and there will be sound and light prompts at the end of program control execution to ensure that operators can timely grasp the operating status of the equipment. In addition, the device also has a temperature deviation limit function, which can set a deviation range of 1-180 ℃ to prevent temperature loss of control.

The correct use of CBS program cooling device is the key to ensuring its long-term stable operation. Before operation, it is necessary to check the liquid nitrogen supply to ensure that the transmission hose is securely connected. Select a suitable freezer according to the sample type and place the samples evenly to ensure the uniformity of heat conduction. Regular maintenance is an important part of ensuring equipment accuracy and lifespan. Temperature sensors should be calibrated regularly to ensure the accuracy of temperature control. Clean the interior of the instrument to prevent residual substances from affecting its performance. At the same time, it is necessary to ensure that the cooling system is working properly and avoid overheating of the instrument due to poor cooling. For equipment that has not been used for a long time, it should be properly packaged and stored to prevent damage from moisture and other environmental factors. Before and after use, check whether the power cord and other connecting lines are normal to avoid electrical faults. It should be noted that there is a specific warranty policy: the warranty period is 1 year and can be extended; During the warranty period, if there is no human error in operation or burning, free maintenance and debugging services will be provided; Charge per use outside the warranty period; Provide on-site technical consultation and free training.