The fully automatic biochemical analyzer is an advanced instrument widely used in clinical testing, which can quickly and accurately analyze the chemical components in a large number of biological samples. The following is an analysis of its basic working principle:
1. Sample loading: Place the blood, urine, and other samples to be tested into the sample tray, and the instrument will use an automatic recognition system to number and record the information of the samples, preparing for accurate detection in the future.
2. Sample addition and reagent allocation: The instrument accurately draws samples and adds them to the reaction vessel through the sample addition system according to the preset program, while adding the corresponding reagents. The sample and reagent quantities are accurately controlled by the computer to ensure consistent conditions for each reaction.
3. Reaction process: After the sample and reagent are fully mixed in the reaction vessel, a chemical reaction occurs, forming a stable detectable substance under certain temperature and time conditions. Different testing items have different chemical reaction principles and conditions, such as enzymatic reactions, immune reactions, etc.
4. Signal detection: After the reaction is completed, the detection system will detect the reaction product. The commonly used detection methods include photoelectric colorimetry, spectrophotometry, etc. Taking the photoelectric colorimetric method as an example, the concentration of the reaction product is determined by measuring the degree of absorption of a specific wavelength of light by the reaction solution, thereby calculating the content of the substance to be tested in the sample.
5. Data processing and result output: The detection system converts the detected signals into electrical signals and transmits them to the computer processing system. The computer analyzes and processes data based on pre-set calibration curves and algorithms, calculates the concentration values of various biochemical indicators in the sample, and displays or prints the detection report on the screen.
Precautions for using a fully automatic biochemical analyzer:
1. Safe operation:
-During the operation, it is essential to comply with safety regulations and avoid contact with harmful substances or sharp objects.
-Use personal protective equipment (such as gloves, goggles, etc.) to protect oneself from potential hazards.
2. Sample processing:
-Ensure that the collection, storage, and transportation of samples comply with regulatory requirements.
-Avoid sample contamination, hemolysis, or coagulation to avoid affecting test results.
3. Reagent management:
-Regularly check the expiration date and storage conditions of reagents to avoid using expired or deteriorated reagents.
4. Instrument maintenance:
-Regularly clean, calibrate, and maintain the analyzer to ensure its performance and accuracy.
-Follow the manufacturer's maintenance guidelines and replace vulnerable parts and consumables in a timely manner.
5. Quality control:
-Implement strict quality control measures, including the use of quality control products, participation in inter laboratory quality evaluations, etc.
-Regularly evaluate the performance indicators of the analyzer (such as precision, accuracy, etc.) to ensure the reliability of the test results.
6. Data recording and reporting:
-Accurately record all data and information during the testing process, including sample information, testing items, results, etc.
-Timely generate and review test reports to ensure their accuracy and completeness.