Tobramycin（Tobramycin）Also known as Topmycin, it is an aminoglycoside antibiotic. Aminoglycoside antibiotics do not contain conjugated structures and have weak UV absorption, making them unsuitable for direct analysis using conventional UV detectors.2020The detection of substances related to tobramycin raw materials in the Chinese Pharmacopoeia is carried out using an evaporative light scattering detector（ELSD），ELSDandCADCompared to low sensitivity and poor repeatability; The microbiological assay method is used for content detection, but the method specificity is poor. 《USP》The current standard uses thin-layer chromatography for the detection of relevant substances in tobramycin, which cannot conduct in-depth analysis of various impurities; The content detection method involves derivatizing the sample and detecting it through a UV detector, which is a cumbersome operation. For this reason, the United States Pharmacopeia Commission2023year1Monthly draft for soliciting opinions^[1], usingHPLC-CADMethod to detect the relevant substances and content of tobramycin.