In modern biotechnology and chemical industry, fermentation process is an important step in producing various products such as antibiotics, enzyme preparations, alcoholic beverages, etc. In order to ensure the optimal growth environment for microorganisms during the fermentation process and avoid external pollution, magnetic stirring glass fermentation tanks have become one of the important equipment. This device combines transparent and visual glass material with efficient magnetic stirring technology, which not only facilitates the observation of changes during the fermentation process, but also provides uniform mixing conditions to promote oxygen transfer, which is particularly important for aerobic fermentation.
1、 Overview of Magnetic Stirring Glass Fermentation Tank
The magnetic stirring glass fermentation tank is mainly composed of the following parts:
Glass tank: It is usually made of high borosilicate glass, which has good corrosion resistance and transparency, making it easy to observe internal reactions.
Magnetic drive system: By using an external motor to drive the built-in permanent magnet rotor to rotate, non-contact mixing operation is achieved, reducing the risk of leakage caused by seal wear.
Temperature control system: including heating device and cooling system, used to maintain a constant temperature required for the fermentation process.
Gas supply system: Provides necessary air or other gases to support aerobic microbial growth, and can accurately control ventilation through flow meters.
PH and dissolved oxygen monitoring system: Real time monitoring of the acidity and dissolved oxygen levels of the fermentation broth to ensure that microorganisms are in the optimal growth environment.
Control system: An intelligent control panel integrated with PLC or DCS system, which can automatically adjust various parameters, record experimental data, and set alarm functions.
2、 Operation steps for use
preparation
Check all connections for tightness, especially the inlet and outlet ports, gas interfaces, and sensor connectors.
Clean the inside and outside of the tank to ensure that there are no residues that may affect the subsequent experimental results.
Prepare the culture medium according to the experimental requirements and add an appropriate amount of inoculum.
Installation and Debugging
Place the magnetic stirrer in the center of the tank bottom, being careful not to let the stirring blades touch the tank wall or bottom.
Connect the power cord, gas pipeline, and other peripheral devices, turn on the control system for preheating testing, and check whether the working status of each component is normal.
Start fermenting
Set the required operating parameters (such as temperature, stirring speed, ventilation rate, etc.), start the magnetic stirrer and temperature control system.
After reaching the set conditions, slowly add the pre prepared culture medium, then close the lid and seal it.
Real time monitoring of key indicators during the fermentation process, adjusting operating parameters according to actual conditions.
End fermentation
When the fermentation cycle is over, stop the gas supply first, then turn off the stirring motor, and finally cut off the heating source.
Open the exhaust valve to release the pressure inside the tank. After the temperature drops to room temperature, open the tank cover to collect the product.
3、 Key points of maintenance and upkeep
Daily cleaning
Immediately clean the inside of the tank after use to prevent residual substances from drying up and becoming difficult to remove.
For glass surfaces, a soft cloth dipped in mild detergent can be gently wiped to avoid scratches.
Regularly inspect the mixing blades for wear or deformation, and replace with new parts if necessary.
periodic calibration
Regularly calibrate the pH electrode and dissolved oxygen probe to ensure measurement accuracy.
Calibration should follow the standard solution and operating instructions provided by the manufacturer to maintain stable instrument performance.
Electrical maintenance
Regularly check whether the wire plugs are damaged or aging, and replace damaged parts in a timely manner.
When not in use for a long time, it is recommended to unplug the power plug and store it in a dry and ventilated place to prevent moisture from causing short circuit faults.
Long term storage precautions
If it is expected that the equipment will not be used for a long time, rust proof oil should be applied to protect the metal components after thorough cleaning and drying.
Properly store various accessories and vulnerable parts for future maintenance and replacement.
In summary, magnetic stirring glass fermentation tanks play an important role in various fields such as biopharmaceuticals and food processing due to their advantages. The correct usage method combined with scientific and reasonable maintenance measures can not only prolong the service life of equipment, but also improve the success rate of experiments, providing more reliable data support for researchers.