The safe usage guidelines for internal standard mixing of 13 β - receptor agonists in methanol are as follows:

1. Storage conditions: It should be stored in a frozen environment, usually at -18 ℃± 5 ℃, and placed in a cool place to avoid high temperatures and direct sunlight. Ice packs should be used during transportation to prevent temperature fluctuations from affecting stability.

2. Preparation of internal standard mixture of 13 β - receptor agonists in methanol before use: After taking it out of the refrigerator, it needs to be equilibrated at room temperature (20 ± 3) ℃ until the temperature stabilizes, and then opened for use. Prevent glass ampoules from breaking or solution properties from changing due to sudden temperature fluctuations.

3. Opening and Sampling: Use a pipette to accurately measure the solution and avoid direct pouring. Once the ampoule is opened, it should be used immediately and cannot be melted again before being used as a standard substance to prevent contamination or concentration changes.

4. Operation protection: Wear a mask, gloves, and protective clothing during operation, and carry out the operation in a fume hood to avoid inhaling volatile gases or skin contact. Methanol is toxic, and beta agonists may cause irritation or allergic reactions in the human body.

5. Disposal of mixed waste of 13 β - receptor agonists in methanol: The used solutions and containers must be disposed of according to laboratory hazardous waste disposal regulations and cannot be discarded at will. Methanol and beta agonists may cause environmental pollution.

6. Equipment and Environment: Ensure that the experimental environment is clean and avoid mixing with other chemicals. When using high-precision analytical instruments, regular calibration is necessary to prevent cross contamination or data bias.

7. Emergency measures: If the solution comes into contact with the skin or eyes, immediately rinse with plenty of water and seek medical assistance. If accidentally inhaled or ingested, seek medical attention immediately and bring the product manual or safety data sheet (SDS) for reference.