1、 Scope of application

Mainly applicable toSpecial dietary foodFor example, sports nutrition foods, special medical formula foods, etc.

2、 Operation process

（1） Phase 1: Sample Preparation and Extraction

Take the test solution and add itSodium carbonate buffer solutionandDansulfonyl chloride derivative reagent, inReact at room temperature and avoid light for 2 hoursTo generate derivatives of the target amino acid that can be detected by UV, the reaction needs to be terminated by adding methylamine hydrochloride solution before the reaction is complete.

(3)Phase Three: Instrumental Analysis

1.First method (HPLC):Filter the derived solution and inject it into a high-performance liquid chromatograph for analysis. The instrument conditions typically include a C18 chromatographic column, gradient elution using disodium hydrogen phosphate and methanol as mobile phases, and a wavelength of 247nmUse a UV detector for detection;

2. Second method (LC-MS/MS):The standard does not provide detailed steps for the second method, but according to the method name, after pretreatment (enzymatic hydrolysis), the sample should not require derivatization and should be directly or appropriately purified before entering a liquid chromatography tandem mass spectrometer for analysis. This method usually has better selectivity and higher sensitivity.

（4） Phase Four: Quantitative Analysis

1. Prepare a series of concentration standard solutions using standard samples of asparagine and glutamine, and perform derivatization (first method) or machine analysis simultaneously with the sample to draw a standard curve;

2. Based on the chromatographic peak area of the target substance in the sample, find the concentration on the standard curve, and then calculate the final content in the sample according to the formula. The calculation result retains three significant digits.

3、 Summary

As a mandatory national standard, this standard has become the legal basis for regulatory authorities to conduct market supervision spot checks, risk monitoring, and determine whether products are qualifiedKey technical support has been provided for special medical conditions such as patients with urea cycle disorders, who require strict restriction of their intake of asparagine and glutamine.Xibiao, as a manufacturer of standard materials, helps GB 5009.309-2025Welcome all major customers to come and purchase!

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