Fully automatic microbial adaptive evolution instrumentIt is a laboratory device used for long-term evolution research of microorganisms, which simulates natural selection pressure to help microbial populations undergo adaptive evolution under controlled conditions.
success cases
Case 1: Directed Evolution of D-Aloulose-3-Isomerase Synthesis
A high-throughput screening platform based on droplet microfluidics (DREM cell) was constructed and combined with a D-aloulose biosensor for the directed evolution of ketose 3-isomerases (KEases); The catalytic efficiency of mutant M3-2 is 17 times higher than that of the original sfDAE.
Case 2: High throughput screening of high-yield glutamine mutant strains
Using a high-throughput skin upgraded droplet microfluidic cell sorter (DREM cell), the whole genome mutation library of Bacillus amyloliquefaciens induced by atmospheric pressure room temperature plasma (ARTP) was subjected to ultra high-throughput screening, and a mutant strain with a glutamine enzyme production increase of over 47% was successfully obtained.
Case 3: Mining of genes associated with efficient secretion proteins of Bacillus subtilis
A genotype phenotype association map of protein secretion was drawn in the cereal stick, systematically revealing the gene loci in its genome that can be used to improve secretion protein production. A chassis strain with a 2.78-fold increase in VHH production was obtained by knocking out the combination of oxygen reducing transcription factors CosR and RshA.
Case 4: Study on the Functional Diversity of Bee Intestinal Microorganisms in Single Cell Culture
This study utilized a high-throughput droplet microfluidic platform (DREM cell) to perform skin upgraded droplet culture and niao gun based metagenomic sequencing on individual bacteria in the bee gut. Through metagenomic analysis, the diversity of bee gut microbiota at the strain level was demonstrated, revealing the existence of potential new strains in different bacterial genera.