Nano high-pressure homogenizerAs the core equipment for preparing nanoscale materials such as nanosuspensions, liposomes, protein formulations, etc., the accuracy of key parameters such as pressure, flow rate, and temperature directly determines the particle size distribution, uniformity, and stability of the materials. To ensure the effectiveness of experiments/production, it is necessary to strictly follow the standardized parameter calibration process. The following is a detailed guide for precise operation:
1、 Preparation before calibration: Basic support is essential
1. Equipment and environmental inspection
ensureNano high-pressure homogenizerIn the shutdown and power-off state, there is no damage to the appearance, and the pipeline connection is firm without leakage; Clean the surface of the equipment and debris on the operating table, keep the environment clean and ventilated, control the temperature at 15-30 ℃ (to avoid temperature fluctuations affecting calibration accuracy), and stay away from interference factors such as strong magnetic fields and vibration sources.
2. Preparation of calibration tools and consumables
Prepare standard instruments that have passed metrological verification, such as precision pressure gauges (with an accuracy level of not less than 0.2), standard flow meters, thermometers, and timers; Prepare the appropriate calibration medium (such as water, glycerin, or corresponding material simulation solution selected according to the routine processing materials of the equipment, to ensure that the medium is clean and free of impurities), sealing gasket, wrench, and other auxiliary tools. At the same time, prepare a calibration record sheet for recording calibration data.
3. Safety protection preparation
Wear protective equipment, including impact goggles, high pressure gloves, and protective clothing, to avoid personal injury caused by sudden pressure changes or medium leaks during the calibration process; Check if the safety interlock devices of the equipment (such as pressure overload protection and emergency stop button) are functioning properly and effectively.
2、 Core parameter calibration steps: precise operation process
1. Pressure parameter calibration (key core parameters)
Pressure is the core factor affecting the nano homogenization effect, and the calibration steps are as follows:
Connecting calibration device: Connect the precision pressure gauge in parallel to the pressure detection port of the homogenizer (or replace the original pressure display unit of the equipment) through an adapter to ensure good sealing and no pressure leakage.
No load test run: Connect the equipment power, start the homogenizer, run it without load for 3-5 minutes, and check if the equipment runs smoothly without any abnormal noise.
Multi point calibration test: Set 3-5 calibration points (such as 50) based on the commonly used pressure range of the equipment MPa、100MPa、150MPa、200MPa、250MPa， Including commonly used work pressure points; Start the pressure output point by point, and after the pressure stabilizes (stay for more than 30 seconds), record the pressure display value of the equipment and the standard value of the precision pressure gauge separately.
Error calculation and adjustment: Calculate the error values of each calibration point (error=device display value - standard value). If the error exceeds the allowable range of the device (usually ≤± 1% FS), it is necessary to correct the display value through the device's calibration menu or adjust the pressure sensor parameters until the error meets the requirements; After calibration is completed, test each point again to confirm that the data is stable.
2. Flow parameter calibration
Flow stability affects material processing efficiency and uniformity. Calibration steps:
Build a flow calibration system: Connect a standard flow meter at the discharge port of the homogenizer, or use the "weighing method" (prepare an electronic balance, beaker, timer, with an accuracy of not less than 0.1g) to ensure smooth pipeline connection and no leakage.
Set calibration conditions: Based on the regular operating parameters of the equipment, set a fixed pressure (taking the commonly used working pressure), start the equipment, and start calibration after the flow rate stabilizes.
Data recording and calculation: If a standard flow meter is used, directly record the displayed flow value of the device and the standard value of the flow meter; If using the weighing method, a beaker is used to receive the discharge, and a timer is started to accurately time for 1 minute. The mass of the material in the beaker is weighed, and the actual flow rate is calculated based on the material density (flow rate=mass/density/time).
Adjustment and verification: When comparing the displayed flow rate of the device with the actual flow rate and the error exceeds ± 2%, the device flow adjustment knob needs to be adjusted or the parameters need to be corrected through the control system. Repeat the verification until the error is qualified.
3. Temperature parameter calibration
The temperature rise of the material during the homogenization process may affect its stability, and the temperature display of the equipment needs to be calibrated:
Place the standard thermometer: Fix the probe of the standard thermometer near the temperature detection point of the equipment (such as the outlet of the homogenization chamber or inside the material pipeline), ensuring that the probe is in full contact with the material and does not affect the flow of the material.
Heating test: Start the device, set different temperature settings (or simulate the temperature under normal working conditions), wait for the temperature to stabilize, and record the displayed temperature of the device and the reading of the standard thermometer.
Correction error: If the temperature error exceeds ± 1 ℃, adjust the parameters through the device temperature calibration function, calibrate again, and ensure accurate temperature display.
3、 Post calibration closure: standardized retention and maintenance
Data recording and archiving: Record the calibration date, calibration personnel, calibration tool information, standard values of each calibration point, equipment display values, error values, adjustment results, etc. in detail in the calibration record sheet, archive and retain them to ensure traceability.
Equipment reset: Remove the calibration device, restore the original pipeline connections of the equipment, and ensure that all joints are sealed firmly; Clean the calibration medium on the surface of the equipment and inside the pipeline to avoid residual effects on subsequent use.
Calibration cycle confirmation: Based on the frequency of equipment use, operating conditions, and measurement requirements, set the calibration cycle (it is recommended to calibrate every 3-6 months, and shorten it to 1-3 months for high-frequency use or harsh operating conditions), and post a calibration qualification label on the equipment, indicating the date of the next calibration.