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Suzhou Micro Flow Nano Biotechnology Co., Ltd
Detailed Explanation of Standard Operating Procedures for Nano Homogenizer
Date: 2025-09-12Read: 1
Nano homogenizerIt is a key equipment for preparing nanoscale suspensions, emulsions, or liposomes, and its operational standardization directly affects the product particle size distribution, stability, and equipment safety. The following is a detailed analysis of Standard Operating Procedures (SOP), covering the entire process of preparation, operation, and maintenance.
1、 Preparation before operation: safety and parameter calibration
1. Environmental and material inspection: Ensure that the laboratory temperature (20-25 ℃) and humidity (≤ 60%) meet the requirements, that the materials to be processed are free of foreign objects and particle agglomeration, and that the uniformity of the pre mixed liquid has been preliminarily tested and meets the standards.
2. Equipment status confirmation: Check that the homogenizer host, high-pressure pump, homogenization valve and other components are not loose or leaking, and that the high-pressure pipeline is firmly connected; After connecting the power supply, verify the normal operation of the control system (such as pressure sensors and motor drives) through a self-test program.
3. Parameter preset: Based on the material characteristics (such as viscosity, density) and target particle size (usually 100-500nm), input initial parameters on the control panel - pressure range (commonly 50-200MPa, nano level needs to be ≥ 150MPa), cycle times (2-5 times), and upper temperature limit (≤ 60 ℃ to prevent material denaturation).
2、 Operation process: precise control in stages
1. Pre cycle stage: Start low-speed stirring or homogenization (pressure 30-50MPa) to initially disperse the material evenly, avoiding direct high pressure that may cause local overheating or valve blockage; Observe whether the feed pump runs smoothly and confirm that there are no bubbles mixed in (bubbles will reduce homogenization efficiency and damage the valve group).
2. High pressure homogenization stage: Gradually increase the pressure to the preset value (such as 180MPa), and the material is compressed by the high-pressure pump and passes through the homogenization valve (micrometer level gap). Under instantaneous pressure release, it undergoes a triple action of "high-speed impact cavity effect shear" to achieve nanoscale crushing. The operator needs to monitor the pressure curve (fluctuation ≤ ± 5MPa) and temperature changes in real time (controlled by jacket cooling water to ≤ 60 ℃).
3. Circulation and monitoring: After a single homogenization, samples are taken and the particle size distribution is detected by a laser particle size analyzer; If the standard is not met (such as D90>500nm), repeat homogenization 1-2 times (total cycle not exceeding 5 times to prevent material overheating and degradation), and record pressure, temperature, and particle size data after each cycle.
3、 Shutdown and Maintenance: Cleaning and Status Preservation
1. Shutdown operation: After homogenization is completed, first reduce the pressure to zero, turn off the high-pressure pump, and after the material is discharged from the pipeline, rinse the pipeline with deionized water or solvent circulation to prevent residual material from solidifying and blocking the valve port.
2. Cleaning and drying: Disassemble parts that can come into contact with materials (such as homogenization valves and feed pipes), clean them with a soft brush and mild cleaning agent to avoid scratching the surface; After cleaning, dry in a 50-60 ℃ oven or blow dry with nitrogen to prevent rusting.
3. Long term storage: If the machine is stopped for more than a week, special lubricating oil should be injected into the high-pressure pump, residual liquid in the pipeline should be drained, and the main engine should be covered with a dust cover to prevent dust and moisture from entering.
Strictly following the above SOP can ensureNano homogenizerStable output of high-precision nanoscale materials, while extending equipment lifespan and ensuring operational safety.