Supercritical fluid chromatography columnIt is a column commonly used in chemical and biological analysis, and plays a crucial role in supercritical fluid chromatography analysis.

The separation of supercritical fluid chromatography columns is based on the synergistic effect of "mobile phase dissolution fixed phase adsorption/distribution", and the specific process is as follows:

Mobile phase characteristics: Supercritical fluids (such as CO ₂ at 31.1 ℃ and above 7.38MPa) have the characteristics of low gas viscosity and high liquid solubility, which can quickly carry samples through chromatographic columns and greatly shorten separation time.

Fixed phase action: The fixed phase (such as silica gel bonded phase) filled in the chromatographic column adsorbs or distributes different components in the sample with varying strengths through polarity differences, hydrophobic interactions, or hydrogen bonding interactions.

Separation process: After the sample enters the chromatographic column with the supercritical mobile phase, the retention time of each component in the column varies due to the different interaction strengths with the stationary phase, and then flows out of the chromatographic column in order to achieve separation.

Note: The separation efficiency can be further optimized by adjusting the pressure and temperature of the mobile phase or adding modifiers (such as methanol, ethanol) to change its solubility and polarity.

　　Supercritical fluid chromatography columnMainly composed of high-pressure pump, chromatographic column, detector, etc. High pressure pumps are used to transport supercritical fluids with no pulses and low flow rates; The chromatographic column is filled with stationary phase material; The detector is used to monitor the composition of the effluent after chromatographic separation. It has the characteristics of high efficiency, green, and low solvent residue. It is easier to achieve higher column efficiency and shorter analysis time than high-performance liquid chromatography.