1. Background Introduction

Huangqi, a traditional Chinese medicinal herb, is the dried root of the leguminous plant Huangqi or Huangqi membranaceus, which has the effect of nourishing qi and blood. Huangqi glycoside IV is the main medicinal active ingredient. There are no strong UV absorbing groups in the chemical structure of Astragaloside IV, only in201nmThere is weak UV end absorption on both sides, and the baseline noise detected by UV detectors is high and the reproducibility is poor. And using a universal quality detectorELSDIt can effectively avoid the defects of ultraviolet detection. This experiment follows the requirements of the Chinese Pharmacopoeia and usesHPLC-ELSDA method for detecting the content of Astragaloside IV in Astragalus membranaceus has been established, which is accurate, sensitive, simple, and rapid, and can effectively achieve quality control of Astragaloside IV in Astragalus membranaceus.

Mobile phase: single pump: acetonitrile: water=36:64, isocratic elution

Flow rate: 1.0 mL/min

Column temperature: 35 ℃